c-Fos Antibody - #AF5354

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Product: c-Fos Antibody
Catalog: AF5354
Description: Rabbit polyclonal antibody to c-Fos
Application: WB IHC IF/ICC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 35~55kD; 41kD(Calculated).
Uniprot: P01100
RRID: AB_2837839

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
c-Fos Antibody detects endogenous levels of total c-Fos.
RRID:
AB_2837839
Cite Format: Affinity Biosciences Cat# AF5354, RRID:AB_2837839.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator protein 1; AP 1; C FOS; Cellular oncogene c fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ murine osteosarcoma viral v fos oncogene homolog; FBJ Osteosarcoma Virus; FOS; FOS protein; FOS_HUMAN; G0 G1 switch regulatory protein 7; G0/G1 switch regulatory protein 7; G0S7; Oncogene FOS; p55; proto oncogene c Fos; Proto oncogene protein c fos; Proto-oncogene c-Fos; v fos FBJ murine osteosarcoma viral oncogene homolog;

Immunogens

Immunogen:
Uniprot:

P01100(FOS_HUMAN) >>Visit HPA database.

Gene(ID):
FOS(2353)
Description:
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.
Sequence:
MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPAPSAGAYSRAGVVKTMTGGRAQSIGRRGKVEQLSPEEEEKRRIRRERNKMAAAKCRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKLEFILAAHRPACKIPDDLGFPEEMSVASLDLTGGLPEVATPESEEAFTLPLLNDPEPKPSVEPVKSISSMELKTEPFDDFLFPASSRPSGSETARSVPDMDLSGSFYAADWEPLHSGSLGMGPMATELEPLCTPVVTCTPSCTAYTSSFVFTYPEADSFPSCAAAHRKGSSSNEPSSDSLSSPTLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
60
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01100 As Substrate

Site PTM Type Enzyme
Y10 Phosphorylation
Y30 Phosphorylation
S32 Phosphorylation Q13164 (MAPK7)
S70 Phosphorylation
K113 Sumoylation
K113 Ubiquitination
T114 Phosphorylation
T116 Phosphorylation
K128 Sumoylation
S133 Phosphorylation
K139 Sumoylation
K176 Sumoylation
K188 Ubiquitination
T232 Phosphorylation Q13164 (MAPK7) , P27361 (MAPK3) , P28482 (MAPK1)
S258 Phosphorylation
S261 Phosphorylation
K265 Sumoylation
S278 Phosphorylation
S308 Phosphorylation O14920 (IKBKB)
T325 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
T331 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S362 Phosphorylation O75676 (RPS6KA4) , Q15418 (RPS6KA1)
S363 Phosphorylation
S374 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T376 Phosphorylation

Research Backgrounds

Function:

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

PTMs:

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).

Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

Subcellular Location:

Nucleus. Endoplasmic reticulum. Cytoplasm>Cytosol.
Note: In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer; with JUN (By similarity). Interacts with MAFB (By similarity). Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site. Interacts with SMAD3; the interaction is weak even on TGF-beta activation. Interacts with MAFB. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with CDS1 and PI4K2A (By similarity).

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

References

1). Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors. BMC Medicine, 2023 (PubMed: 36600274) [IF=9.3]

Application: IF/ICC    Species: Rat    Sample: hippocampal

Fig. 2 Prolonged anesthesia inducing neuroinflammation, upregulating NF-κB inflammatory pathway, downregulating neuronal excitability, and inactivating apoptotic signaling. A, B TNF-α, IL-1β, and IL-6 evidently increased in the cortex (A) and hippocampus (B) after prolonged anesthesia (n = 4 or 5 per group). C Effects of prolonged anesthesia on the morphological changes of neurons in the hippocampus. Scale bar = 20 μm. D Prolonged anesthesia activated NF-κB inflammatory pathway (n = 3 per group). E The number of Phospho-NF-κB P65-colocalized nuclei in the hippocampus (n = 4 per group). Scale bar = 10 μm. F Prolonged anesthesia inhibiting neuronal excitability marker C-fos expression in the hippocampal CA1 region (n = 4 per group). Scale bar = 20 μm. G Exhibiting representative EEG raw traces (upper) and power spectrograms (bottom) for the hippocampus. H Prolonged anesthesia triggered burst suppression in the hippocampus (n = 6 per group). The burst suppression ratio (BSR) was calculated as the percentage of suppression time of each 1 min binary series. I TUNEL staining in brain slices was negative after prolonged anesthesia. Scale bar = 100 μm. J The number of Nissl’s body (n = 4 per group). Scale bar = 20 μm. K Prolonged anesthesia had no effect on apoptotic pathways (n = 3 per group). L, M Quantification of Cleaved caspase-3/caspase-3 (L) and bcl-2/bax (M) levels normalized to β-actin. Data was shown as Mean ± SD, with *P < 0.05 or *P < 0.001; Sevo group vs. control group. Arrows represent positive cells or colocalization
2). Effects and Mechanisms of Rhus chinensis Mill. Fruits on Suppressing RANKL-Induced Osteoclastogenesis by Network Pharmacology and Validation in RAW264.7 Cells. Nutrients, 2022 (PubMed: 35267996) [IF=5.9]

Application: WB    Species:    Sample: RAW264.7 cells

Figure 9.| Effects of the ethanolic extract from the R. chinensis fruits on RANKL-induced osteoclastogenesis on c-Fos and NFATc1 proteins in RAW264.7 cells. (a) Western blot analysis of c-Fos and proteins
3). JNK signaling pathway mediates acetaminophen-induced hepatotoxicity accompanied by changes of glutathione S-transferase A1 content and expression. Frontiers in Pharmacology, 2019 (PubMed: 31620005) [IF=5.6]

Application: WB    Species: mouse    Sample: liver

FIGURE 2 | Activation of JNK signaling pathway under different dosages of APAP. (A) Western blot analyses of total tissue lysate for p-JNK, JNK, p-c-Jun, c-Jun,p-c-Fos, c-Fos, and β-actin (loading control).
4). Dehydromiltirone inhibits osteoclast differentiation in RAW264.7 and bone marrow macrophages by modulating MAPK and NF-κB activity. Frontiers in Pharmacology, 2022 (PubMed: 36210855) [IF=5.6]
5). Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage. International journal of molecular sciences, 2023 (PubMed: 37762273) [IF=5.6]

Application: WB    Species: Mouse    Sample:

Figure 7 MAPK/AP-1 signaling pathway. (A) is the representative blots. (B–K) are the ratios of JNK, ERK, P38, Jun, c-FOS, p-JNK, p-ERK, p-P38, p-Jun and p-c-FOS to GAPDH (n = 3, ## p < 0.01 vs. the NCS group and * p < 0.05, ** p < 0.01 vs. the MCS group and ns is no significance).
6). Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine. Chinese Medicine, 2021 (PubMed: 34600579) [IF=4.9]
7). Phosphorylation at Ser 727 Increases STAT3 Interaction with PKCε Regulating Neuron–Glia Crosstalk via IL-6-Mediated Hyperalgesia In Vivo and In Vitro. MEDIATORS OF INFLAMMATION, 2022 (PubMed: 35125963) [IF=4.6]

Application: WB    Species: Rat    Sample: spinal cords

Figure 5 Expression of proteins related to activated neurocytes detected by Western blot analysis. (a–i) Protein expression of pSTAT3 (Ser727), IL-6, c-Fos, GFAP, and Iba-1 in the spinal cords of FCA-treated rats significant decreased (P < 0.05) after intrathecal injections of PKCε inhibitor peptide (100 μg/50 μL), APTSTAT3-9R (20 μg/50 μL), and anti-IL-6 antibody (100 ng/50 μL). Values were normalized against GAPDH and are expressed as ratios (%) of control values. Data are shown as means ± SD (n = 4–5). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; one-way ANOVA followed by Bonferroni tests.
8). Schistosoma japonicum cystatin suppresses osteoclastogenesis via manipulating the NF‑κB signaling pathway. Molecular Medicine Reports, 2021 (PubMed: 33576450) [IF=3.4]
9). Identification and analysis of oxidative stress-related genes in hypoxic-ischemic brain damage using bioinformatics and experimental verification. Immunity, inflammation and disease, 2024 (PubMed: 39172048) [IF=3.2]

Application: IHC    Species: Rat    Sample: brain

Figure 8. Confirmation of the expression of optimal OS‐DEGs in rat HIBD model. (A): The RT‐qPCR results of Jun, Fos, Tlr2 and Atf3 in rat tissues (n = 3). (B): Immunohistochemistry validation of Jun, Fos, Tlr2 and Atf3 in rat samples (n = 3). **(p ≤ .01), *(p ≤ .05). The scales are 20 μm. HIBD, hypoxic‐ischemic brain damage; OS‐DEGs, oxidative stress‐related DEGs; RT‐qPCR, quantitative reverse transcription polymerase chain reaction.
10). Sophoraflavanone G Inhibits RANKL-Induced Osteoclastogenesis via MAPK/NF-κB Signaling Pathway. Molecular biotechnology, 2024 (PubMed: 38780825) [IF=2.6]
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