Phospho-NFAT1 (Ser53) Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-NFAT1 (Ser53) Antibody.

Product:	Phospho-NFAT1 (Ser53) Antibody
Catalog:	AF3882
Description:	Rabbit polyclonal antibody to Phospho-NFAT1 (Ser53)
Application:	WB
Reactivity:	Human
Mol.Wt.:	100-150kD; 100kD(Calculated).
Uniprot:	Q13469
RRID:	AB_2847196

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-NFAT1 (Ser53) Antibody detects endogenous levels of NFAT1 only when phosphorylated at Ser53.

RRID:

AB_2847196
Cite Format: Affinity Biosciences Cat# AF3882, RRID:AB_2847196.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI607462; cytoplasmic 2; KIAA0611; NF ATc2; NF ATp; NF-ATc2; NF-ATp; NFAC2_HUMAN; NFAT 1; NFAT pre existing subunit; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2; Nuclear factor of activated T cells pre-existing component; Nuclear factor of activated T cells, preexisting component; Nuclear factor of activated T-cells; Preexisting nuclear factor of activated T cells 2; T cell transcription factor NFAT 1; T-cell transcription factor NFAT1;

Immunogens

Immunogen:

A synthesized peptide derived from human NFAT1 around the phosphorylation site of Ser53.

Uniprot:

Q13469(NFAC2_HUMAN) >>Visit HPA database.

Gene(ID):

NFATC2(4773)

Expression:

Q13469 NFAC2_HUMAN:

Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.

Sequence:

Q13469 NFAC2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNAPERQPQPDGGDAPGHEPGGSPQDELDFSILFDYEYLNPNEEEPNAHKVASPPSGPAYPDDVLDYGLKPYSPLASLSGEPPGRFGEPDRVGPQKFLSAAKPAGASGLSPRIEITPSHELIQAVGPLRMRDAGLLVEQPPLAGVAASPRFTLPVPGFEGYREPLCLSPASSGSSASFISDTFSPYTSPCVSPNNGGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVALPPGASPQRSRSPSPQPSSHVAPQDHGSPAGYPPVAGSAVIMDALNSLATDSPCGIPPKMWKTSPDPSPVSAAPSKAGLPRHIYPAVEFLGPCEQGERRNSAPESILLVPPTWPKPLVPAIPICSIPVTASLPPLEWPLSSQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIECSQRSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKVVFTEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKHIRTPVKVNFYVINGKRKRSQPQHFTYHPVPAIKTEPTDEYDPTLICSPTHGGLGSQPYYPQHPMVAESPSCLVATMAPCQQFRTGLSSPDARYQQQNPAAVLYQRSKSLSPSLLGYQQPALMAAPLSLADAHRSVLVHAGSQGQSSALLHPSPTNQQASPVIHYSPTNQQLRCGSHQEFQHIMYCENFAPGTTRPGPPPVSQGQRLSPGSYPTVIQQQNATSQRAAKNGPPVSDQKEVLPAGVTIKQEQNLDQTYLDDVNEIIRKEFSGPPARNQT

PTMs - Q13469 As Substrate

Q13469

Site	PTM Type	Enzyme	Source
S23	Phosphorylation		Uniprot
S31	Phosphorylation	Q86Y07 (VRK2)	Uniprot
Y36	Phosphorylation		Uniprot
Y38	Phosphorylation		Uniprot
S53	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S56	Phosphorylation	Q05513 (PRKCZ)	Uniprot
K70	Ubiquitination		Uniprot
S73	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
K96	Ubiquitination		Uniprot
S99	Phosphorylation		Uniprot
K102	Ubiquitination		Uniprot
S107	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
T116	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
R150	Methylation		Uniprot
S171	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
S174	Phosphorylation		Uniprot
S175	Phosphorylation		Uniprot
S177	Phosphorylation		Uniprot
Y212	Phosphorylation		Uniprot
S213	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
S225	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
S236	Phosphorylation		Uniprot
S243	Phosphorylation		Uniprot
S245	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
S255	Phosphorylation		Uniprot
S268	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
S276	Phosphorylation		Uniprot
S280	Phosphorylation		Uniprot
T325	Phosphorylation		Uniprot
S326	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
S337	Phosphorylation		Uniprot
K338	Ubiquitination		Uniprot
Y346	Phosphorylation		Uniprot
S363	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
T374	Phosphorylation		Uniprot
K482	Ubiquitination		Uniprot
T483	Phosphorylation		Uniprot
T485	Phosphorylation		Uniprot
K491	Ubiquitination		Uniprot
K497	Ubiquitination		Uniprot
K520	Ubiquitination		Uniprot
K530	Ubiquitination		Uniprot
T585	Phosphorylation		Uniprot
T599	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
T652	Phosphorylation		Uniprot
S736	Phosphorylation		Uniprot
S737	Phosphorylation		Uniprot
Y752	Phosphorylation		Uniprot
S755	Phosphorylation		Uniprot
S757	Phosphorylation		Uniprot
S759	Phosphorylation		Uniprot
S761	Phosphorylation		Uniprot
S776	Phosphorylation		Uniprot
S790	Phosphorylation		Uniprot
S801	Phosphorylation		Uniprot
S808	Phosphorylation		Uniprot
S814	Phosphorylation		Uniprot
S856	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
Y860	Phosphorylation		Uniprot
S882	Phosphorylation		Uniprot
K885	Ubiquitination		Uniprot
T893	Phosphorylation		Uniprot
K895	Sumoylation		Uniprot
Y904	Phosphorylation		Uniprot

Research Backgrounds

Q13469_NFAC2_HUMAN

Function:

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

PTMs:

In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.

Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

Tissue Specificity:

Subunit Structure:

Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP (By similarity). Interacts with FOXP3. Interacts with TBX21 ('Thr-303' phosphorylated form) (By similarity). Interacts with KAT2A (By similarity). Interacts with HOMER2 and HOMER3; this interaction competes with calcineurin/PPP3CA-binding and hence prevents NFATC2 dephosphorylation and activation. Interacts with protein phosphatase PPP3CA/calcineurin A.

Family&Domains:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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Phospho-NFAT1 (Ser53) Antibody - #AF3882