Phospho-STAT4 (Tyr693) Antibody | Affinity Biosciences

WB
IHC
pElisa
Cites

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Phospho-STAT4 (Tyr693) Antibody - Western blot analysis of Phospho-STAT4 (Tyr693) expression in Mouse heart tissue lysate.

Phospho-STAT4 (Tyr693) Antibody - AF3441 at 1/200 staining human kidney tissue sections by IHC-P.

Phospho-STAT4 (Tyr693) Antibody - peptide-ELISA analysis of AF3441.

Phospho-STAT4 (Tyr693) Antibody - Figure 4 BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway.

Product:	Phospho-STAT4 (Tyr693) Antibody
Catalog:	AF3441
Description:	Rabbit polyclonal antibody to Phospho-STAT4 (Tyr693)
Application:	WB IHC IF/ICC IP
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit, Chicken
Mol.Wt.:	86kDa; 86kD(Calculated).
Uniprot:	Q14765
RRID:	AB_2834883

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Rabbit(100%), Chicken(89%)

Clonality:

Polyclonal

Specificity:

Phospho-STAT4 (Tyr693) Antibody detects endogenous levels of STAT4 only when phosphorylated at Tyrosine 693.

RRID:

AB_2834883
Cite Format: Affinity Biosciences Cat# AF3441, RRID:AB_2834883.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human STAT4 around the phosphorylation site of Tyr693.

Uniprot:

Q14765(STAT4_HUMAN) >>Visit HPA database.

Gene(ID):

STAT4(6775)

Description:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

Sequence:

Q14765 STAT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Rabbit

100

Chicken

Bovine

Sheep

Dog

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q14765_STAT4_HUMAN

Function:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

PTMs:

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

References

1). Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats. Aging-US, 2021 (PubMed: 33793419) [IF=3.9]

Application: WB Species: Rat Sample: fibroblasts

Figure 4 BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. (A) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. (B) The expression of three most significant DEGs (POSTN, COMP, and TGFBI) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). (C) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. (D) The percentage of migration area in different groups. (E) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. (F) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-STAT4 (Tyr693) Antibody - #AF3441