Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] Antibody - #AF3304

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Product: Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] Antibody
Catalog: AF3304
Description: Rabbit polyclonal antibody to Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731]
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 90kDa; 91kD,90kD(Calculated).
Uniprot: P42229 | P51692
RRID: AB_2834723

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-STAT5 (Ser725/Ser730) Antibody detects endogenous levels of STAT5 only when phosphorylated at Ser726/Ser731, which site historically referenced as Ser725/Ser730.
RRID:
AB_2834723
Cite Format: Affinity Biosciences Cat# AF3304, RRID:AB_2834723.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Mammary gland factor; MGF; Signal transducer and activator of transcription 5A; STA5A_HUMAN; STAT 5; STAT 5A; STAT5; STAT5A; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5; Stat5b; Transcription factor STAT5B;

Immunogens

Immunogen:
Uniprot:

P42229(STA5A_HUMAN) >>Visit HPA database.

P51692(STA5B_HUMAN) >>Visit HPA database.

Gene(ID):
STAT5B(6777) | STAT5A(6776)
Description:
STAT5B transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases triggered by cytokines including IL2, IL3, GM-CSF, and various growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression.
Sequence:
MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS

MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS

PTMs - P42229/P51692 As Substrate

Site PTM Type Enzyme
Y22 Phosphorylation
K70 Ubiquitination
K71 Ubiquitination
K84 Acetylation
K84 Ubiquitination
K86 Ubiquitination
Y90 Phosphorylation
T92 O-Glycosylation
T92 Phosphorylation
Y114 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
K163 Ubiquitination
K164 Ubiquitination
S193 Phosphorylation
K207 Ubiquitination
K232 Ubiquitination
K235 Ubiquitination
K242 Ubiquitination
T245 Phosphorylation
K256 Sumoylation
K256 Ubiquitination
K336 Sumoylation
K343 Ubiquitination
K359 Ubiquitination
K370 Ubiquitination
T372 Phosphorylation
S375 Phosphorylation
K380 Ubiquitination
K384 Acetylation
K384 Sumoylation
K384 Ubiquitination
S473 Phosphorylation
K516 Sumoylation
K516 Ubiquitination
K527 Ubiquitination
K586 Ubiquitination
K644 Ubiquitination
T647 Phosphorylation
T648 Phosphorylation
Y668 Phosphorylation
K675 Ubiquitination
K681 Ubiquitination
Y682 Phosphorylation
Y683 Phosphorylation
K689 Ubiquitination
Y694 Phosphorylation Q06187 (BTK) , P12931 (SRC) , P52333 (JAK3) , P06239 (LCK) , P06213 (INSR) , P07948 (LYN) , P43405 (SYK) , Q13882 (PTK6) , O60674 (JAK2) , P08631 (HCK)
K696 Acetylation
K696 Sumoylation
K696 Ubiquitination
K700 Acetylation
Y720 Phosphorylation
S726 Phosphorylation
T757 Phosphorylation
S774 Phosphorylation
S777 Phosphorylation
S780 Phosphorylation Q13153 (PAK1) , P28482 (MAPK1) , P27361 (MAPK3)
S792 Phosphorylation
S794 Phosphorylation
Site PTM Type Enzyme
K56 Sumoylation
K70 Ubiquitination
K71 Ubiquitination
K84 Acetylation
K84 Ubiquitination
K86 Ubiquitination
Y90 Phosphorylation
T92 Phosphorylation
Y98 Phosphorylation
Y114 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
S132 Phosphorylation
K140 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
S193 Phosphorylation
K207 Ubiquitination
K232 Ubiquitination
K235 Ubiquitination
K242 Ubiquitination
T245 Phosphorylation
K256 Sumoylation
K256 Ubiquitination
K336 Sumoylation
K343 Ubiquitination
K359 Ubiquitination
K370 Ubiquitination
T372 Phosphorylation
S375 Phosphorylation
K380 Ubiquitination
K384 Acetylation
K384 Sumoylation
K384 Ubiquitination
Y392 Phosphorylation
K516 Sumoylation
K516 Ubiquitination
K527 Ubiquitination
K583 Ubiquitination
K586 Ubiquitination
S655 Phosphorylation
Y665 Phosphorylation
Y668 Phosphorylation
K675 Sumoylation
K675 Ubiquitination
Y679 Phosphorylation P12931 (SRC)
K681 Acetylation
K681 Ubiquitination
Y682 Phosphorylation
Y683 Phosphorylation
T684 Phosphorylation
C688 S-Nitrosylation
T692 Phosphorylation
K694 Acetylation
K694 Ubiquitination
Y699 Phosphorylation Q13882 (PTK6) , P06213 (INSR) , P08631 (HCK) , P12931 (SRC) , P00533 (EGFR) , O60674 (JAK2)
K701 Acetylation
K701 Sumoylation
K701 Ubiquitination
K705 Acetylation
Y725 Phosphorylation P00533 (EGFR)
S731 Phosphorylation
Y740 Phosphorylation P00533 (EGFR)
Y743 Phosphorylation P00533 (EGFR)
S787 Phosphorylation

Research Backgrounds

Function:

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

PTMs:

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694.

ISGylated.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7. Interacts with ERBB4.

Family&Domains:

Belongs to the transcription factor STAT family.

Function:

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.

PTMs:

Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1. Interacts with NMI. Interacts with SOCS7. Interacts (via SH2 domain) with INSR. Interacts with CPEB3; this inhibits STAT5B-mediated transcriptional activation.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

References

1). Eldecalcitol effectively prevents alveolar bone loss by partially improving Th17/Treg cell balance in diabetes-associated periodontitis. Frontiers in Bioengineering and Biotechnology, 2023 [IF=5.7]

Application: WB    Species: Mouse    Sample:

FIGURE 7. ELD regulated Th17/Treg cells through the STAT3/STAT5 signal. (A) (a) The protein expression of p-STAT3, STAT3, p-STAT5, STAT5 and GAPDH detected by western blotting in the PD, PD + ELD, DPD and DPD + ELD groups. The statistical analysis of (b) p-STAT3/STAT3, (c) p-STAT5/STAT5 and (d) the ratio of p-STAT3/p-STAT5 in western blotting. (B) The representative immunofluorescence staining images of p-STAT3+IL-17A+ cells in the (a) PD, (b) PD + ELD, (c) DPD and (d) DPD + ELD groups. (e) The statistical analysis of p-STAT3+IL-17A+ cell counts. The representative immunofluorescence staining images of p-STAT5+FOXP3+ cells in the (f) PD, (g) PD + ELD, (h) DPD and (i) DPD + ELD groups. (j) The statistical analysis of p-STAT5+FOXP3+ cell counts. (k) The ratio of p-STAT3+IL-17A+/p-STAT5+FOXP3+ cells.

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 7. ELD regulated Th17/Treg cells through the STAT3/STAT5 signal. (A) (a) The protein expression of p-STAT3, STAT3, p-STAT5, STAT5 and GAPDH detected by western blotting in the PD, PD + ELD, DPD and DPD + ELD groups. The statistical analysis of (b) p-STAT3/STAT3, (c) p-STAT5/STAT5 and (d) the ratio of p-STAT3/p-STAT5 in western blotting. (B) The representative immunofluorescence staining images of p-STAT3+IL-17A+ cells in the (a) PD, (b) PD + ELD, (c) DPD and (d) DPD + ELD groups. (e) The statistical analysis of p-STAT3+IL-17A+ cell counts. The representative immunofluorescence staining images of p-STAT5+FOXP3+ cells in the (f) PD, (g) PD + ELD, (h) DPD and (i) DPD + ELD groups. (j) The statistical analysis of p-STAT5+FOXP3+ cell counts. (k) The ratio of p-STAT3+IL-17A+/p-STAT5+FOXP3+ cells.
2). You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2020 (PubMed: 31526861) [IF=5.4]

Application: IHC    Species: rat    Sample: kidney

Fig. 7.| Effect of the YGY on the HIF1α-STAT5 pathway in the kidney of CKD rats with reproductive dysfunction. (A–D) Expression of EPO (A), p-EPOR (Tyr368) (B),p-JAK2 (Tyr570) (C), and p-STAT5 (Ser725) (D) were determined via immunohistochemistry. The presence of these proteins is indicated by brown staining as observed via light microscopy. (A1, B1, C1, D1) kidneys of the NC group rats; (A2, B2, C2, D2) kidneys of the CKD group rats; (A3, B3, C3, D3) kidneys of the GL 10 g kg−1 group rats; (A4, B4, C4, D4) kidneys of the YGY 10 g kg−1 group rats; (A5, B5, C5, D5)kidneys of the YGY 20 g kg−1 group rats; (A6, B6, C6, D6) kidneys of the YGY40 g•kg−1 group rats (Bar = 50 μm, 200 × , the red arrow refers to the brown staining). Quantitative data are shown in the right panel.

Application: WB    Species: rat    Sample: kidney

Fig. 7.| Effect of the YGY on the HIF1α-STAT5 pathway in the kidney of CKD rats with reproductive dysfunction. (E) Western blotting bands for kidney HIF1α, EPO, p-EPOR (Tyr368), total EPOR, p-JAK2 (Tyr570), total JAK2, p-STAT5 (Ser725) and total STAT5 proteins, and quantitative data are shown in the lower panel. Data are presented as the mean ± SD. (n=3). *P < 0.05, **P < 0.01 compared with the NC group. #P < 0.05, ##P < 0.01 compared with the CKD group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
3). Shengmaisan combined with Liuwei Dihuang Decoction alleviates chronic intermittent hypoxia-induced cognitive impairment by activating the EPO/EPOR/JAK2 signaling pathway. Chinese journal of natural medicines, 2024 (PubMed: 38796216) [IF=4.6]
4). Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). European Journal of Histochemistry : EJH, 2019 (PubMed: 30652434) [IF=2.0]

Application: IHC    Species: rat    Sample: glandular cells, epithelial cells and interstitial cells

Figure 3. | Seasonal immunolocalization of PRL, PRLR, STAT5 and pSTAT5 in the scented glands of the muskrats. Black arrows indicatethe three cell types: glandular cells, epithelial cells and interstitial cells. The first column (a,e,i,m) represents staining in the breedingseason. The second column (b,f,j,o) represent staining in the non-breeding season. The third (c, g, k, p) and fourth (d, h, l, q) columnsrepresent staining in the positive and negative control, respectively. a,b) Immunolocalization of PRL in the scented glands. e,f)Immunolocalization of PRLR in the scented glands. i,j) Immunolocalization of STAT5 in the scented glands. m,o) Immunolocalizationof pSTAT5 in the scented glands. PRL, PRLR, STAT5 and pSTAT5 detected in the mammary glands were used as a positive control(c,g,k,p). Negative control sections were treated with normal rabbit serum instead of primary antisera (d,h,l,q). B, breeding season; NB,non-breeding season; PC, positive control; NC, negative control; GC, glandular cell; EC, epithelial cell; IC, interstitial cell. Scale bars:25 μm.
5). Seasonal patterns of prolactin, prolactin receptor, and STAT5 expression in the ovaries of wild ground squirrels (Citellus dauricus Brandt). European Journal of Histochemistry, 2023 (PubMed: 37781865) [IF=2.0]

Application: IHC    Species: Mouse    Sample:

Figure 2.Immunohistochemical staining of PRL, PRLR, STAT, and p-STAT5 in the ovaries of wild ground squirrels during the breeding and non-breeding seasons. Positive staining of PRL (a,b), PRLR (d,e), STAT5 (g,h) and p-STAT5 (j,k) were detected in the GC, LC and IC of ovaries during the breeding season. The staining of PRL (c), PRLR (f), STAT5 (i) and p-STAT5 (l) were weak during the non-breeding season. No immunostaining was detected in the negative control sections (m-o). B, breeding season; NB, non-breeding season; GC, granulosa cells; TC, theca cells; IC, interstitial cells; LC, luteal cells; NC, negative control.
6). Sertoli Cells - Methods and Protocols. European Journal of Histochemistry : EJH, 2019

Application: IHC    Species: muskrats    Sample:

Figure 3. Seasonal immunolocalization of PRL, PRLR, STAT5 and pSTAT5 in the scented glands of the muskrats. Black arrows indicate the three cell types: glandular cells, epithelial cells and interstitial cells. The first column (a,e,i,m) represents staining in the breeding season. The second column (b,f,j,o) represent staining in the non-breeding season. The third (c, g, k, p) and fourth (d, h, l, q) columns represent staining in the positive and negative control, respectively. a,b) Immunolocalization of PRL in the scented glands. e,f) Immunolocalization of PRLR in the scented glands. i,j) Immunolocalization of STAT5 in the scented glands. m,o) Immunolocalization of pSTAT5 in the scented glands. PRL, PRLR, STAT5 and pSTAT5 detected in the mammary glands were used as a positive control (c,g,k,p). Negative control sections were treated with normal rabbit serum instead of primary antisera (d,h,l,q). B, breeding season; NB, non-breeding season; PC, positive control; NC, negative control; GC, glandular cell; EC, epithelial cell; IC, interstitial cell. Scale bars: 25 μm.

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