INGR2 Antibody | Affinity Biosciences

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Western blot analysis INGR2 using HeLa whole cell lysates.

Product:	INGR2 Antibody
Catalog:	DF8988
Description:	Rabbit polyclonal antibody to INGR2
Application:	WB
Reactivity:	Human
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	38 kDa; 38kD(Calculated).
Uniprot:	P38484
RRID:	AB_2842184

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(92%), Sheep(83%), Rabbit(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

INGR2 Antibody detects endogenous levels of total INGR2.

RRID:

AB_2842184
Cite Format: Affinity Biosciences Cat# DF8988, RRID:AB_2842184.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AF 1; AF-1; AF1; IFGR 2; IFGR2; IFN-gamma receptor 2; IFN-gamma-R2; IFNGR 2; IFNGR2; IFNGT 1; IFNGT1; IMD28; INGR2_HUMAN; Interferon gamma receptor 2 (interferon gamma transducer 1); Interferon gamma receptor 2; Interferon gamma receptor accessory factor 1; Interferon gamma receptor beta chain; Interferon gamma receptor chain 2; Interferon gamma transducer 1;

Immunogens

Immunogen:

Uniprot:

P38484(INGR2_HUMAN) >>Visit HPA database.

Gene(ID):

IFNGR2(3460)

Expression:

P38484 INGR2_HUMAN:

Expressed in T-cells (at protein level).

Sequence:

P38484 INGR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRPTLLWSLLLLLGVFAAAAAAPPDPLSQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYQVQFKYTDSKWFTADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNISCYETMADASTELQQVILISVGTFSLLSVLAGACFFLVLKYRGLIKYWFHTPPSIPLQIEEYLKDPTQPILEALDKDSSPKDDVWDSVSIISFPEKEQEDVLQTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Dog

Sheep

Rabbit

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38484 As Substrate

P38484

Site	PTM Type	Source
N56	N-Glycosylation	Uniprot
N85	N-Glycosylation	Uniprot
N110	N-Glycosylation	Uniprot
N137	N-Glycosylation	Uniprot
S198	Phosphorylation	Uniprot
N219	N-Glycosylation	Uniprot
N231	N-Glycosylation	Uniprot
K308	Ubiquitination	Uniprot
S324	Phosphorylation	Uniprot
T336	Phosphorylation	Uniprot

Research Backgrounds

P38484_INGR2_HUMAN

Function:

Associates with IFNGR1 to form a receptor for the cytokine interferon gamma (IFNG). Ligand binding stimulates activation of the JAK/STAT signaling pathway. Required for signal transduction in contrast to other receptor subunit responsible for ligand binding.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass type I membrane protein. Endoplasmic reticulum membrane>Single-pass type I membrane protein. Cytoplasm.
Note: Has low cell surface expression and high cytoplasmic expression in T cells. The bias towards cytoplasmic expression may be due to ligand-independent receptor internalization and recycling.

Tissue Specificity:

Expressed in T-cells (at protein level).

Subunit Structure:

Heterodimer with IFNGR1, to form the IFNG receptor complex. Interacts (via intracellular domain) with JAK2.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

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INGR2 Antibody - #DF8988