Product: Phospho-PKC gamma (Thr514) Antibody
Catalog: AF8347
Description: Rabbit polyclonal antibody to Phospho-PKC gamma (Thr514)
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 75 KD; 78kD(Calculated).
Uniprot: P05129
RRID: AB_2840409

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(86%), Horse(100%), Sheep(86%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PKC gamma (Thr514) Antibody detects endogenous levels of PKC gamma only when phosphorylated at Thr514.
RRID:
AB_2840409
Cite Format: Affinity Biosciences Cat# AF8347, RRID:AB_2840409.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KPCG_HUMAN; MGC57564; OTTHUMP00000067291; PKC-gamma; PKCC; PKCG; PRKCG; Protein kinase C gamma; Protein kinase C gamma polypeptide; Protein kinase C gamma type; Protein kinase C, gamma; SCA 14; SCA14;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P05129 KPCG_HUMAN:

Expressed in Purkinje cells of the cerebellar cortex.

Sequence:
MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
86
Sheep
86
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05129 As Substrate

Site PTM Type Enzyme
S109 Phosphorylation
S145 Phosphorylation
S148 Phosphorylation
T155 Phosphorylation
Y195 Phosphorylation
K197 Acetylation
K197 Ubiquitination
S264 Phosphorylation
S320 Phosphorylation
S342 Phosphorylation
K359 Acetylation
K359 Ubiquitination
S361 Phosphorylation
K503 Acetylation
T510 Phosphorylation
T511 Phosphorylation
T512 Phosphorylation
T514 Phosphorylation
T518 Phosphorylation
Y521 Phosphorylation
Y529 Phosphorylation
T655 Phosphorylation
T674 Phosphorylation

PTMs - P05129 As Enzyme

Substrate Site Source
O94768 (STK17B) S351 Uniprot
P04004 (VTN) S381 Uniprot
P04049 (RAF1) S497 Uniprot
P04049 (RAF1) S619 Uniprot
P04083 (ANXA1) S27 Uniprot
P06127 (CD5) T434 Uniprot
P06127 (CD5) T436 Uniprot
P06730-1 (EIF4E) S209 Uniprot
P07900 (HSP90AA1) T115 Uniprot
P07900 (HSP90AA1) T425 Uniprot
P07900 (HSP90AA1) T603 Uniprot
P08138 (NGFR) S277 Uniprot
P10636-8 (MAPT) S258 Uniprot
P10636-8 (MAPT) S293 Uniprot
P10636-8 (MAPT) S324 Uniprot
P10636-8 (MAPT) S352 Uniprot
P11388-1 (TOP2A) S29 Uniprot
P17302 (GJA1) S368 Uniprot
P19429 (TNNI3) S42 Uniprot
P19429 (TNNI3) S44 Uniprot
P21730 (C5AR1) S334 Uniprot
P25098 (GRK2) S29 Uniprot
P28329-3 (CHAT) T255 Uniprot
P28329-3 (CHAT) S346 Uniprot
P28329-3 (CHAT) S347 Uniprot
P28329 (CHAT) T373 Uniprot
P28329-3 (CHAT) S440 Uniprot
P28329 (CHAT) S464 Uniprot
P28329 (CHAT) S465 Uniprot
P28329-3 (CHAT) S476 Uniprot
P28329 (CHAT) S558 Uniprot
P28329 (CHAT) S594 Uniprot
P29474 (NOS3) T495 Uniprot
P30086 (PEBP1) S153 Uniprot
P34741 (SDC2) S187 Uniprot
P34741 (SDC2) S188 Uniprot
P35568 (IRS1) S1101 Uniprot
P41180 (CASR) T888 Uniprot
P41594-2 (GRM5) S840 Uniprot
P43629 (KIR3DL1) S415 Uniprot
P48058 (GRIA4) S862 Uniprot
P49619 (DGKG) S779 Uniprot
P49619 (DGKG) S782 Uniprot
P49768 (PSEN1) S346 Uniprot
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
P50281 (MMP14) T567 Uniprot
P61764-2 (STXBP1) S306 Uniprot
P61764-1 (STXBP1) S313 Uniprot
P98082 (DAB2) S24 Uniprot
Q03721 (KCNC4) S8 Uniprot
Q03721 (KCNC4) S9 Uniprot
Q03721 (KCNC4) S15 Uniprot
Q03721 (KCNC4) S21 Uniprot
Q05586 (GRIN1) S890 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q13224 (GRIN2B) S1323 Uniprot
Q14155 (ARHGEF7) S518 Uniprot
Q14155 (ARHGEF7) S761 Uniprot
Q16625-1 (OCLN) S340 Uniprot
Q5JVS0-1 (HABP4) T354 Uniprot
Q5JVS0 (HABP4) T375 Uniprot
Q7Z2E3 (APTX) T125 Uniprot
Q92686 (NRGN) S36 Uniprot
Q99418-2 (CYTH2) S391 Uniprot
Q99418-1 (CYTH2) S392 Uniprot
Q9UBS0 (RPS6KB2) S473 Uniprot
Q9UQ80-1 (PA2G4) S363 Uniprot
Q9UQ80-1 (PA2G4) T366 Uniprot

Research Backgrounds

Function:

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity).

PTMs:

Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4.

Ubiquitinated.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Cell membrane>Peripheral membrane protein. Cell junction>Synapse>Synaptosome. Cell projection>Dendrite.
Note: Translocates to synaptic membranes on stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in Purkinje cells of the cerebellar cortex.

Subunit Structure:

Interacts with GRIA4 (By similarity). Interacts with CDCP1. Interacts with TP53INP1 and p53/TP53. Interacts with ARNTL/BMAL1 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Metformin Inhibits Tumor Metastasis through Suppressing Hsp90α Secretion in an AMPKα1-PKCγ Dependent Manner. Cells, 2020 (PubMed: 31936169) [IF=6.0]

Application: WB    Species: human    Sample: H1299-WT, KD and OV cells

Figure 6. |AMPKα1 inhibits Hsp90α phosphorylation, membrane translocation and secretion by suppressing the kinase activity of PKCγ. pT514-PKCγ, PKCγ and actin were measured by Western blot in H1299-WT, KD and OV cells (A) and MCF-7-WT, KD and OV cells (B) The level of pT514 is an indicator of PKCγ activation.

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