DBH Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse testis, using DBH Antibody at 1/1000 dilution.

DF7060 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

Product:	DBH Antibody
Catalog:	DF7060
Description:	Rabbit polyclonal antibody to DBH
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Chicken, Xenopus
Mol.Wt.:	60-80kDa; 69kD(Calculated).
Uniprot:	P09172
RRID:	AB_2839016

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(100%), Horse(89%), Chicken(89%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

DBH Antibody detects endogenous levels of total DBH.

RRID:

AB_2839016
Cite Format: Affinity Biosciences Cat# DF7060, RRID:AB_2839016.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dbh; DBM; Dopamine beta hydroxylase; Dopamine beta monooxygenase; Dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine beta-monooxygenase; DOPO_HUMAN; OTTHUMP00000022501; Soluble dopamine beta-hydroxylase;

Immunogens

Immunogen:

Uniprot:

P09172(DOPO_HUMAN) >>Visit HPA database.

Gene(ID):

DBH(1621)

Description:

Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8).

Sequence:

P09172 DOPO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPALSRWASLPGPSMREAAFMYSTAVAIFLVILVAALQGSAPRESPLPYHIPLDPEGSLELSWNVSYTQEAIHFQLLVRRLKAGVLFGMSDRGELENADLVVLWTDGDTAYFADAWSDQKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEVFQCAPEMDSVPHFSGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Xenopus

100

Pig

Horse

Chicken

Dog

Zebrafish

Sheep

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09172 As Substrate

P09172

Site	PTM Type	Source
N64	N-Glycosylation	Uniprot
N184	N-Glycosylation	Uniprot
Y310	Phosphorylation	Uniprot
Y327	Phosphorylation	Uniprot
Y334	Phosphorylation	Uniprot
Y353	Phosphorylation	Uniprot
T422	Phosphorylation	Uniprot
N566	N-Glycosylation	Uniprot
S587	Phosphorylation	Uniprot
T588	Phosphorylation	Uniprot
S603	Phosphorylation	Uniprot

Research Backgrounds

P09172_DOPO_HUMAN

Function:

Conversion of dopamine to noradrenaline.

PTMs:

N-glycosylated.

Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle lumen. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule lumen. Secreted.

Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass type II membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass type II membrane protein.

Subunit Structure:

Homotetramer; composed of two disulfide-linked dimers.

Family&Domains:

Belongs to the copper type II ascorbate-dependent monooxygenase family.

Research Fields

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. Immunity, 2023 (PubMed: 36854305) [IF=32.4]

2). Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor. BMC neuroscience, 2024 (PubMed: 38291397) [IF=2.4]

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DBH Antibody - #DF7060