Product: DBH Antibody
Catalog: DF7060
Description: Rabbit polyclonal antibody to DBH
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Chicken, Xenopus
Mol.Wt.: 60-80kDa; 69kD(Calculated).
Uniprot: P09172
RRID: AB_2839016

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(100%), Horse(89%), Chicken(89%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
DBH Antibody detects endogenous levels of total DBH.
RRID:
AB_2839016
Cite Format: Affinity Biosciences Cat# DF7060, RRID:AB_2839016.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dbh; DBM; Dopamine beta hydroxylase; Dopamine beta monooxygenase; Dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine beta-monooxygenase; DOPO_HUMAN; OTTHUMP00000022501; Soluble dopamine beta-hydroxylase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8).
Sequence:
MPALSRWASLPGPSMREAAFMYSTAVAIFLVILVAALQGSAPRESPLPYHIPLDPEGSLELSWNVSYTQEAIHFQLLVRRLKAGVLFGMSDRGELENADLVVLWTDGDTAYFADAWSDQKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEVFQCAPEMDSVPHFSGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Xenopus
100
Pig
89
Horse
89
Chicken
89
Dog
78
Zebrafish
50
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09172 As Substrate

Site PTM Type Enzyme
N64 N-Glycosylation
N184 N-Glycosylation
Y310 Phosphorylation
Y327 Phosphorylation
Y334 Phosphorylation
Y353 Phosphorylation
T422 Phosphorylation
N566 N-Glycosylation
S587 Phosphorylation
T588 Phosphorylation
S603 Phosphorylation

Research Backgrounds

Function:

Conversion of dopamine to noradrenaline.

PTMs:

N-glycosylated.

Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle lumen. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule lumen. Secreted.

Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass type II membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer; composed of two disulfide-linked dimers.

Family&Domains:

Belongs to the copper type II ascorbate-dependent monooxygenase family.

Research Fields

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. Immunity, 2023 (PubMed: 36854305) [IF=32.4]

2). Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor. BMC neuroscience, 2024 (PubMed: 38291397) [IF=2.4]

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