Caspase 3 Antibody | Affinity Biosciences

Cites

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FIGURE 2 Overexpression of miR27a in nucleus pulposus cells inhibits apoptosis and the production of inflammatory cytokines.

FIGURE 2 GDF11 inhibited apoptosis of DGR in vitro.

Product:	Caspase 3 Antibody
Catalog:	DF6879
Description:	Rabbit polyclonal antibody to Caspase 3
Application:	WB IF/ICC IP
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	17kDa,19kDa,35kDa; 32kD(Calculated).
Uniprot:	P42574
RRID:	AB_2838838

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IP 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Bovine(100%), Horse(86%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)

Clonality:

Polyclonal

Specificity:

Caspase 3 Antibody detects endogenous levels of total Caspase 3.

RRID:

AB_2838838
Cite Format: Affinity Biosciences Cat# DF6879, RRID:AB_2838838.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A830040C14Rik; Apopain; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3, apoptosis-related cysteine peptidase; Caspase 3, apoptosis-related cysteine protease; Caspase 3, apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama;

Immunogens

Immunogen:

Uniprot:

P42574(CASP3_HUMAN) >>Visit HPA database.

Gene(ID):

CASP3(836)

Expression:

P42574 CASP3_HUMAN:

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Description:

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

Sequence:

P42574 CASP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42574 As Substrate

P42574

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
T4	Phosphorylation		Uniprot
S7	Phosphorylation		Uniprot
S10	Phosphorylation		Uniprot
K11	Acetylation		Uniprot
K11	Ubiquitination		Uniprot
S12	Phosphorylation		Uniprot
K14	Ubiquitination		Uniprot
K19	Ubiquitination		Uniprot
S24	Phosphorylation		Uniprot
S26	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
Y41	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
S65	Phosphorylation		Uniprot
T67	Phosphorylation		Uniprot
K82	Acetylation		Uniprot
K82	Ubiquitination		Uniprot
K88	Ubiquitination		Uniprot
K105	Ubiquitination		Uniprot
K138	Ubiquitination		Uniprot
S150	Phosphorylation	Q16539 (MAPK14)	Uniprot
T152	Phosphorylation		Uniprot
C163	S-Nitrosylation		Uniprot
T174	Phosphorylation		Uniprot
S176	Phosphorylation		Uniprot
K210	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
S249	Phosphorylation		Uniprot
K260	Ubiquitination		Uniprot
T270	Phosphorylation		Uniprot

Research Backgrounds

P42574_CASP3_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

PTMs:

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 33248852) [IF=6.7]

Application: WB Species: human Sample: HCT-116 cells

Figure 10. (A) Compound P-3 triggered the explosion of ROS in HCT-116 cells. (magnification 20 ×, scale bar = 100 μm) (B) P-3 regulated the expression of proteins involved in Cyto-c/Caspase3 pathway. Data are presented as mean ± SD from three independent experiments. * P < 0.05, ** P<0.01, *** P<0.001 versus the control group.

2). The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis. Frontiers in Bioengineering and Biotechnology, 2022 (PubMed: 35059389) [IF=5.7]

Application: WB Species: Rat Sample: DGR Cells

FIGURE 2 GDF11 inhibited apoptosis of DGR in vitro. (A and B). Flow cytometry was used to detect the degree of apoptosis of DRG after cultured with recombinant GDF11 for 3 days. (C, D and E). DRG were cultured with recombinant GDF11 for 3 days, and mRNA expression of apoptosis-related genes Bcl2, Bax and caspase3 were detected by RT-PCR. (F, G, H and I). After DRG were cultured with recombinant GDF11 for 3 days, the protein expressions of apoptosis-related genes Bcl2, Bax and caspase3 were detected by Western blot. Statistical analysis was based on at least three different biological samples and three technical replicates, p < 0.05 was considered to be statistically significant.

3). TNF‐α induces up‐regulation of MicroRNA‐27a via the P38 signalling pathway, which inhibits intervertebral disc degeneration by targeting FSTL1. Journal of Cellular and Molecular Medicine, 2021 (PubMed: 34190406) [IF=5.3]

Application: WB Species: Human Sample: NP cells

FIGURE 2 Overexpression of miR27a in nucleus pulposus cells inhibits apoptosis and the production of inflammatory cytokines. (A) The transfection efficiency of miR27a as detected by qPCR. (B, C, D, E and F) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, COX2 expression was detected by immunofluorescence (B, C), qPCR (D) and Western blotting (E, F). (E, G, H, I) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, Bcl2, Bax and caspase 3 expression was detected by Western blotting. (J, K) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, apoptosis was detected by flow cytometry. Statistical analysis was based on at least three different biological samples and three technical replicates; P < .05 was considered to be statistically significant. Representative images are shown

4). miR‑576‑3p overexpression enhances cisplatin sensitivity of ovarian cancer cells by dysregulating PD‑L1 and cyclin D1. Molecular Medicine Reports, 2021 (PubMed: 33236151) [IF=3.4]

5). Knockdown of lncRNA MIR503HG suppresses proliferation and promotes apoptosis of non-small cell lung cancer cells by regulating miR-489-3p and miR-625-5p. PATHOLOGY RESEARCH AND PRACTICE, 2020 (PubMed: 31983569) [IF=2.8]

Application: WB Species: Human Sample: A549 and NCI-H2170 cells

Fig. 3. Knockdown of MIR503HG promoted the apoptosis of NSCLC cells. MIR503HG shRNA or NC shRNA were transfected in A549 and NCI-H2170 cells, respectively. (A–B) The apoptosis of A549 and NCI-H2170 cells was determined after transfection 48 h using TUNEL staining, Scale bar =50 μm. (C–F) The apoptosis of A549 and NCI-H2170 cells was detected by flow cytometry with Annexin Ⅴ/PI staining after transfection 48 h. (G–H) The protein levels of Bax, Bad, Bcl-2, Survivin, caspase-3, cleaved-caspase-3, caspase-9 and cleaved-caspase-9 were determined by western blot after transfection 48 h. The data were presented as mean ± SD and significant differences were indicated as **P < 0.01

6). Schisandrin A protects intestinal cells from mycophenolic acid-induced cytotoxicity and oxidative damage. Research Square, 2021

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Caspase 3 Antibody - #DF6879