HIST2H2BE Antibody - #DF6718

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Product: HIST2H2BE Antibody
Catalog: DF6718
Description: Rabbit polyclonal antibody to HIST2H2BE
Application: WB IHC
Reactivity: Human
Mol.Wt.: 14kDa; 14kD(Calculated).
Uniprot: Q16778
RRID: AB_2838680

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
HIST2H2BE Antibody detects endogenous levels of total HIST2H2BE.
RRID:
AB_2838680
Cite Format: Affinity Biosciences Cat# DF6718, RRID:AB_2838680.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GL105; H2B; H2B histone family member Q; H2B-GL105; H2B.1; H2B/q; H2B2E_HUMAN; H2BFQ; H2BGL105; H2BQ; HIST2H2BE; Histone 2 H2be; histone cluster 2, H2be; histone H2B GL105; histone H2B type 2 E; Histone H2B type 2-E; histone H2B type 2E; Histone H2B-GL105; Histone H2B.q; histone H2BGL105;

Immunogens

Immunogen:

A synthesized peptide derived from human HIST2H2BE, corresponding to a region within C-terminal amino acids.

Uniprot:

Q16778(H2B2E_HUMAN) >>Visit HPA database.

Gene(ID):
HIST2H2BE(8349)
Description:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Sequence:
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

PTMs - Q16778 As Substrate

Site PTM Type Enzyme
Acetylation
M1 Acetylation
K6 Acetylation
K6 Methylation
K6 Sumoylation
K6 Ubiquitination
S7 Phosphorylation
K12 Acetylation
K13 Acetylation
S15 Phosphorylation
K16 Acetylation
K17 Acetylation
K21 Acetylation
K21 Sumoylation
K21 Ubiquitination
K24 Acetylation
K25 Acetylation
K35 Ubiquitination
S37 Phosphorylation
Y38 Phosphorylation
S39 Phosphorylation
Y41 Phosphorylation
Y43 Phosphorylation
K44 Acetylation
K44 Ubiquitination
K47 Acetylation
K47 Methylation
K47 Sumoylation
K47 Ubiquitination
T53 Phosphorylation
S56 Phosphorylation
S57 Phosphorylation
K58 Acetylation
K58 Methylation
K58 Ubiquitination
S65 Phosphorylation
R73 Methylation
S79 Phosphorylation
R80 Methylation
Y84 Phosphorylation
K86 Acetylation
K86 Ubiquitination
R87 Methylation
S88 Phosphorylation
T89 Phosphorylation
T91 Phosphorylation
S92 Phosphorylation
R93 Methylation
T97 Phosphorylation
R100 Methylation
K109 Acetylation
K109 Methylation
K109 Sumoylation
K109 Ubiquitination
S113 Phosphorylation
T116 Phosphorylation
K117 Acetylation
K117 Sumoylation
K117 Ubiquitination
T120 Phosphorylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
Y122 Phosphorylation
T123 Phosphorylation
S124 Phosphorylation
K126 Acetylation
K126 Ubiquitination

Research Backgrounds

Function:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

PTMs:

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.

Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.

GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).

Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Family&Domains:

Belongs to the histone H2B family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

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