IL2RG Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat, using IL2RG antibody.

Product:	IL2RG Antibody
Catalog:	DF6642
Description:	Rabbit polyclonal antibody to IL2RG
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	42kDa; 42kD(Calculated).
Uniprot:	P31785
RRID:	AB_2838604

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(100%), Sheep(100%), Dog(90%)

Clonality:

Polyclonal

Specificity:

IL2RG Antibody detects endogenous levels of total IL2RG.

RRID:

AB_2838604
Cite Format: Affinity Biosciences Cat# DF6642, RRID:AB_2838604.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD132; CD132 antigen; CIDX; common cytokine receptor gamma chain; Cytokine receptor common subunit gamma; Gamma C; gamma(c); gammaC; IL-2 receptor subunit gamma; IL-2R gamma chain; IL-2R subunit gamma; IL-2RG; Il2rg; IL2RG_HUMAN; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; p64; SCIDX; SCIDX1;

Immunogens

Immunogen:

Uniprot:

P31785(IL2RG_HUMAN) >>Visit HPA database.

Gene(ID):

IL2RG(3561)

Description:

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder. [provided by RefSeq, Mar 2010]

Sequence:

P31785 IL2RG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLKPSLPFTSLLFLQLPLLGVGLNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPFLFALEAVVISVGSMGLIISLLCVYFWLERTMPRIPTLKNLEDLVTEYHGNFSAWSGVSKGLAESLQPDYSERLCLVSEIPPKGGALGEGPGASPCNQHSPYWAPPCYTLKPET

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Pig

Dog

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31785 As Substrate

P31785

Site	PTM Type	Source
N71	N-Glycosylation	Uniprot
N84	N-Glycosylation	Uniprot
K101	Ubiquitination	Uniprot
K119	Ubiquitination	Uniprot
K147	Ubiquitination	Uniprot
N159	N-Glycosylation	Uniprot
K164	Ubiquitination	Uniprot
K207	Ubiquitination	Uniprot
K217	Ubiquitination	Uniprot
T292	Phosphorylation	Uniprot
K294	Ubiquitination	Uniprot
T301	Phosphorylation	Uniprot
Y303	Phosphorylation	Uniprot
S308	Phosphorylation	Uniprot
K315	Ubiquitination	Uniprot
S320	Phosphorylation	Uniprot
Y325	Phosphorylation	Uniprot
S326	Phosphorylation	Uniprot
Y357	Phosphorylation	Uniprot
Y363	Phosphorylation	Uniprot

Research Backgrounds

P31785_IL2RG_HUMAN

Function:

Common subunit for the receptors for a variety of interleukins. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell surface.

Subunit Structure:

The gamma subunit is common to the IL2, IL4, IL7, IL15, IL21 and probably also the IL13 receptors. Interacts with SHB upon interleukin stimulation.

(Microbial infection) Interacts with HTLV-1 accessory protein p12I.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

References

1). Alleviating CB2-Dependent ER Stress and Mitochondrial Dysfunction Improves Chronic Cerebral Hypoperfusion-Induced Cognitive Impairment. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 2024 (PubMed: 38214766) [IF=6.2]

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IL2RG Antibody - #DF6642