IFNAR1 Antibody | Affinity Biosciences

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Product:	IFNAR1 Antibody
Catalog:	DF6571
Description:	Rabbit polyclonal antibody to IFNAR1
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	70,100,150kD; 64kD(Calculated).
Uniprot:	P17181
RRID:	AB_2838533

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

IFNAR1 Antibody detects endogenous levels of total IFNAR1.

RRID:

AB_2838533
Cite Format: Affinity Biosciences Cat# DF6571, RRID:AB_2838533.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha type antiviral protein; Antiviral protein, alpha-type; Antiviral protein, beta-type; AVP; Beta type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFN alpha REC; IFN alpha receptor; IFN alpha/beta Receptor alpha; IFN beta receptor; IFN Interferon-beta receptor; IFN-alpha/beta receptor 1; IFN-R-1; IFNAR; Ifnar1; IFNBR; IFRC; INAR1_HUMAN; Interferon (alpha beta and omega) receptor 1; interferon alpha and beta receptor subunit 1; Interferon alpha/beta receptor 1; Interferon alpha/beta receptor alpha chain; Interferon beta receptor 1; interferon receptor 1; Interferon-alpha receptor; Type I interferon receptor 1;

Immunogens

Immunogen:

Uniprot:

P17181(INAR1_HUMAN) >>Visit HPA database.

Gene(ID):

IFNAR1(3454)

Expression:

P17181 INAR1_HUMAN:

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.

Description:

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.

Sequence:

P17181 INAR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQPLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV

PTMs - P17181 As Substrate

P17181

Site	PTM Type	Enzyme	Source
N58	N-Glycosylation		Uniprot
T85	Phosphorylation		Uniprot
K86	Ubiquitination		Uniprot
K101	Ubiquitination		Uniprot
N110	N-Glycosylation		Uniprot
K125	Ubiquitination		Uniprot
N172	N-Glycosylation		Uniprot
K191	Ubiquitination		Uniprot
K278	Ubiquitination		Uniprot
N313	N-Glycosylation		Uniprot
K397	Ubiquitination		Uniprot
K414	Ubiquitination		Uniprot
K417	Ubiquitination		Uniprot
Y466	Phosphorylation	P29597 (TYK2)	Uniprot
Y481	Phosphorylation	P29597 (TYK2)	Uniprot
T494	Phosphorylation		Uniprot
S495	Phosphorylation		Uniprot
K501	Ubiquitination		Uniprot
K525	Ubiquitination		Uniprot
K526	Ubiquitination		Uniprot
S532	Phosphorylation	Q16539 (MAPK14)	Uniprot
S535	Phosphorylation	P48729 (CSNK1A1) , Q9BZL6 (PRKD2)	Uniprot
Y538	Phosphorylation		Uniprot
S539	Phosphorylation		Uniprot

Research Backgrounds

P17181_INAR1_HUMAN

Function:

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).

PTMs:

Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.

Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.

Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Late endosome. Lysosome.
Note: Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.

Tissue Specificity:

Subunit Structure:

Heterodimer with IFNAR2. Interacts with STAT1 and STAT2; the interaction requires its phosphorylation at Tyr-466. Interacts with TYK2. Interacts (serine-phosphorylated form) with FBXW11, the substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Interacts with SHMT2; this promotes interaction with ABRAXAS2 and the BRISC complex.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

References

1). USP39 Serves as a Deubiquitinase to Stabilize STAT1 and Sustains Type I IFN–Induced Antiviral Immunity. JOURNAL OF IMMUNOLOGY, 2020 (PubMed: 33127822) [IF=4.4]

Application: WB Species: Sample: 293T cells

FIGURE 4. | USP39 interacts with STAT1.(B) 293T cells transfected with either control siRNA (con siRNA) or siUSP39 were stimulated with IFN-a(1000 IU/ml) for 0, 30, and 60 min. Cell lysates were analyzed by immunoblotting as indicated.

Application: WB Species: Human Sample: 293T cells

FIGURE 4. USP39 interacts with STAT1. (A) 293T cells were transfected with either control siRNA or siUSP39, and mRNA levels of type I IFN molecules were measured by real-time PCR. (B) 293T cells transfected with either control siRNA (con siRNA) or siUSP39 were stimulated with IFN-a (1000 IU/ml) for 0, 30, and 60 min. Cell lysates were analyzed by immunoblotting as indicated. (C) 293T cells were transfected with either vector or Flag- USP39. After 48 h, the protein level of STAT1 was measured by Western blot. (D) 293T cells were transfected with Flag-USP39 plasmid. Forty-eight hours posttransfection, the cells were lysed and immunoprecipitated by STAT1 Ab and protein A agarose beads, The USP39 was analyzed using anti-Flag Ab. Data are from three experiments are mean and SD of three independent replicates (A) or representative of three independent experiments (B–D). A p value .0.05 was considered ns.

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IFNAR1 Antibody - #DF6571