ATP6AP1 Antibody | Affinity Biosciences

WB
IF/ICC

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Western blot analysis of extracts from MCF7, using ATP6AP1 Antibody.

Product:	ATP6AP1 Antibody
Catalog:	DF6341
Description:	Rabbit polyclonal antibody to ATP6AP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	52kDa; 52kD(Calculated).
Uniprot:	Q15904
RRID:	AB_2838305

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ATP6AP1 Antibody detects endogenous levels of total ATP6AP1.

RRID:

AB_2838305
Cite Format: Affinity Biosciences Cat# DF6341, RRID:AB_2838305.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

16A; Ac45; ATP6IP1; ATP6S1; ATPase H+ transporting lysosomal (vacuolar proton pump) subunit 1; ATPase H+ transporting lysosomal accessory protein 1; ATPase H+ transporting lysosomal interacting protein 1; CF2; H ATPase subunit; MGC129781; ORF; Protein XAP 3; Protein XAP3; V ATPase Ac45 subunit; V ATPase S1 accessory protein; V ATPase subunit S1; V type proton ATPase subunit S1; Vacuolar ATP synthase subunit S1; Vacuolar proton pump subunit S1; VATPS1; XAP 3; XAP3;

Immunogens

Immunogen:

Uniprot:

Q15904(VAS1_HUMAN) >>Visit HPA database.

Gene(ID):

ATP6AP1(537)

Expression:

Q15904 VAS1_HUMAN:

widely expressed, with highest levels in brain and lowest in liver and duodenum.

Description:

This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq, Jul 2008]

Sequence:

Q15904 VAS1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMAAMATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQLLQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIWMGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15904 As Substrate

Q15904

Site	PTM Type	Source
T7	Phosphorylation	Uniprot
T184	O-Glycosylation	Uniprot
K211	Ubiquitination	Uniprot
T218	Phosphorylation	Uniprot
N261	N-Glycosylation	Uniprot
N273	N-Glycosylation	Uniprot
T319	Phosphorylation	Uniprot
T320	Phosphorylation	Uniprot
R330	Methylation	Uniprot
K380	Ubiquitination	Uniprot
K460	Ubiquitination	Uniprot
S465	Phosphorylation	Uniprot

Research Backgrounds

Q15904_VAS1_HUMAN

Function:

Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.

PTMs:

N-glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane.
Note: Not detected in trans-Golgi network.

Tissue Specificity:

widely expressed, with highest levels in brain and lowest in liver and duodenum.

Subunit Structure:

Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump. Interacts (via N-terminus) with ATP6AP2 (via N-terminus).

Family&Domains:

Belongs to the vacuolar ATPase subunit S1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

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ATP6AP1 Antibody - #DF6341