CD86 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/10

Western blot analysis of extracts from Mouse testis, using CD86 Antibody at 1/1000 dilution.

Western blot analysis of extracts from EC304 cells, using CD86 Antibody at 1/1000 dilution.

DF6332 staining A549 cells by IF/ICC.

Fig.

Emodin contributed to inhibition of the microglia activation.

Figure 11.

Figure 6.

Figure 8.

Figure 6 (A) Immunohistochemistry of CD138, CD86, and PD-L1.

Figure 7.

Product:	CD86 Antibody
Catalog:	DF6332
Description:	Rabbit polyclonal antibody to CD86
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	38kDa,60-85kD; 38kD(Calculated).
Uniprot:	P42081
RRID:	AB_2838296

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

CD86 Antibody detects endogenous levels of total CD86.

RRID:

AB_2838296
Cite Format: Affinity Biosciences Cat# DF6332, RRID:AB_2838296.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activation B7-2 antigen 3; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen; CD86 molecule; CD86_HUMAN; CLS1; CTLA-4 counter-receptor B7.2 2, 3; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; FUN-1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2;

Immunogens

Immunogen:

Uniprot:

P42081(CD86_HUMAN) >>Visit HPA database.

Gene(ID):

CD86(942)

Expression:

P42081 CD86_HUMAN:

Expressed by activated B-lymphocytes and monocytes.

Description:

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Sequence:

P42081 CD86_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSYKCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF

PTMs - P42081 As Substrate

P42081

Site	PTM Type	Source
S77	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
Y108	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
K315	Acetylation	Uniprot

Research Backgrounds

P42081_CD86_HUMAN

Function:

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

(Microbial infection) Acts as a receptor for adenovirus subgroup B.

PTMs:

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed by activated B-lymphocytes and monocytes.

Subunit Structure:

Homodimer. Interacts with MARCH8.

(Microbial infection) Interacts with adenovirus subgroup b fiber protein.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Allograft rejection.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production. (View pathway)

References

1). Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection. Small, 2022 (PubMed: 35859534) [IF=13.3]

2). Bacterial Toxin‐Responsive Biomimetic Nanobubbles for Precision Photodynamic Therapy against Bacterial Infections. Advanced Healthcare Materials, 2022 (PubMed: 35836329) [IF=10.0]

3). Ganoderma lucidum polysaccharide hydrogel accelerates diabetic wound healing by regulating macrophage polarization. International journal of biological macromolecules, 2024 (PubMed: 38266851) [IF=8.2]

4). Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles. DRUG DELIVERY, 2022 (PubMed: 35642917) [IF=6.0]

Application: WB Species: Mice Sample: Y79 tumors

Figure 11. (A, B) The contents of CD86 in Y79 tumors after different treatments. (C) TNF-α levels in Y79 tumors after different treatments (**p<.01).

5). Recombinant fibroblast growth factor 4 ameliorates axonal regeneration and functional recovery in acute spinal cord injury through altering microglia/macrophage phenotype. International immunopharmacology, 2024 (PubMed: 38728880) [IF=5.6]

6). Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. International Immunopharmacology, 2022 (PubMed: 35176589) [IF=5.6]

7). Zanubrutinib ameliorates lipopolysaccharide-induced acute lung injury via regulating macrophage polarization. International Immunopharmacology, 2022 (PubMed: 35973369) [IF=5.6]

8). miR-146a-5p enhances embryo survival in unexplained recurrent spontaneous abortion by promoting M2 polarization of decidual macrophages. International Immunopharmacology, 2022 (PubMed: 35764020) [IF=5.6]

9). Vascular endothelial growth factor A is a potential prognostic biomarker and correlates with immune cell infiltration in hepatocellular carcinoma. Journal of Cellular and Molecular Medicine, 2023 (PubMed: 36729917) [IF=5.3]

10). Contribution of Oxidative Stress Induced by Sonodynamic Therapy to the Calcium Homeostasis Imbalance Enhances Macrophage Infiltration in Glioma Cells. Cancers, 2022 (PubMed: 35454942) [IF=5.2]

Application: IF/ICC Species: Mouse Sample: brain

Figure 8. (A) GL261 glioma-bearing C57BL/6 to undergo xenograft tumor and brain treatment and MRI tests on days 0 and 24. (B,D) At the end of the experiment (30 days), the mouse brains were made into sections for immunofluorescence staining (CD86 and CD206); (C,E) analysis of the immunofluorescence intensity showed that the SG group induced more CD86 expression in the gliomas than CD206 expression.

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For Research Use Only.
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CD86 Antibody - #DF6332