CD86 Antibody - #DF6332

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Product: CD86 Antibody
Catalog: DF6332
Description: Rabbit polyclonal antibody to CD86
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 38kDa,60-85kD; 38kD(Calculated).
Uniprot: P42081
RRID: AB_2838296

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CD86 Antibody detects endogenous levels of total CD86.
RRID:
AB_2838296
Cite Format: Affinity Biosciences Cat# DF6332, RRID:AB_2838296.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activation B7-2 antigen 3; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen; CD86 molecule; CD86_HUMAN; CLS1; CTLA-4 counter-receptor B7.2 2, 3; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; FUN-1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2;

Immunogens

Immunogen:
Uniprot:

P42081(CD86_HUMAN) >>Visit HPA database.

Gene(ID):
CD86(942)
Expression:
P42081 CD86_HUMAN:

Expressed by activated B-lymphocytes and monocytes.

Description:
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Sequence:
MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSYKCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF

PTMs - P42081 As Substrate

Site PTM Type Enzyme
S77 Phosphorylation
S80 Phosphorylation
Y108 Phosphorylation
T175 Phosphorylation
K315 Acetylation

Research Backgrounds

Function:

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

(Microbial infection) Acts as a receptor for adenovirus subgroup B.

PTMs:

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by activated B-lymphocytes and monocytes.

Subunit Structure:

Homodimer. Interacts with MARCH8.

(Microbial infection) Interacts with adenovirus subgroup b fiber protein.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Allograft rejection.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

References

1). Zou H et al. Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles. DRUG DELIVERY 2022 Dec;29(1):1785-1799. (PubMed: 35642917) [IF=6.0]

Application: WB    Species: Mice    Sample: Y79 tumors

Figure 11. (A, B) The contents of CD86 in Y79 tumors after different treatments. (C) TNF-α levels in Y79 tumors after different treatments (**p<.01).
2). Qiao G et al. Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. International Immunopharmacology 2022 Feb 14;106:108609. (PubMed: 35176589) [IF=5.6]
3). Li X et al. Zanubrutinib ameliorates lipopolysaccharide-induced acute lung injury via regulating macrophage polarization. International Immunopharmacology 2022 Aug 13;111:109138. (PubMed: 35973369) [IF=5.6]
4). Ye HX et al. miR-146a-5p enhances embryo survival in unexplained recurrent spontaneous abortion by promoting M2 polarization of decidual macrophages. International Immunopharmacology 2022 Sep;110:108930. (PubMed: 35764020) [IF=5.6]
5). Qi Y et al. Vascular endothelial growth factor A is a potential prognostic biomarker and correlates with immune cell infiltration in hepatocellular carcinoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2023 Feb 02; (PubMed: 36729917) [IF=5.3]
6). Zhong L et al. Erythropoietin-Induced Autophagy Protects Against Spinal Cord Injury and Improves Neurological Function via the Extracellular-Regulated Protein Kinase Signaling Pathway. MOLECULAR NEUROBIOLOGY 2020 Jul 9. (PubMed: 32647973) [IF=5.1]

Application: IF/ICC    Species: rat    Sample:

Fig. 6 |EPO benefit antiinflammation and axonal regeneration can be neutralized by ERK inhibition, and activator can mimic the effect of EPO. a The axonal special protein GAP43 immunofluorescence staining and histogram at 14 days (magnification × 400). b–e The inflammatory marker proteins immunofluorescence staining and histograms at 14 days, including CD86, GFAP, TNF-α, iNOS(magnification × 400). All data presented as mean ± SEM in each group. *p < 0.05 vs. SCI + saline group, **p < 0.01 vs. SCI + saline group. #p < 0.05 comparison between SCI + EPO group and SCI + EPO + PD98059, ##p < 0.01 comparison between SCI + EPO group and SCI + EPO + PD98059
7). Kenan Zheng et al. Protecting effect of emodin in experimental autoimmune encephalomyelitis mice by inhibiting microglia activation and inflammation via Myd88/PI3K/Akt/NF-κB signalling pathway. Bioengineered 2022 Mar 14. (PubMed: 35287559) [IF=4.9]

Application: IF/ICC    Species: Mice    Sample: brain tissue

Emodin contributed to inhibition of the microglia activation. Double immunofluorescence staining of brain tissue with M1(CD86+/iba1+) (a) and M2(CD206+/iba1+) (b). The representative double immunofluorescence labeling image of CD86 (red)/Iba1 (green) in brain tissue, and the nuclei were stained with DAPI (blue). The representative double immunofluorescence labeling image of CD206 (red)/Iba1 (green) in brain tissue and the nuclei were stained with DAPI (blue). The quantity of the percentage of CD86+/Iba1+ cell (c) and CD206+/Iba1+ cell. (d) The effects of emodin on M1 microglia cell subsets mRNA relative expression. Mice were sacrificed on 21 dpi. The CNS tissues are collected. The IL-6 (e), TGF-β (f), IL-17A (g), and RORγt mRNA relative expression were analyzed by qRT-PCR, respectively. All data were expressed as mean ± S.D (n = 5). Comparisons among each group were analyzed by one-way ANOVA in C, D, F, and G. Comparisons among each group were analyzed by Kruskal-Wallis test using Bonferroni comparisons post hoc tests in E and H. *P < 0.05, **P < 0.01 versus NC group, #P < 0.05, ##P < 0.01 versus EAE group.
8). Niu J et al. Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Frontiers in Oncology 2020 Aug 21;10:1628. (PubMed: 32974202) [IF=4.7]
9). Chen Y et al. Rubicon promotes the M2 polarization of Kupffer cells via LC3-associated phagocytosis-mediated clearance to improve liver transplantation. CELLULAR IMMUNOLOGY 2022 May 30;378:104556. (PubMed: 35700602) [IF=4.3]
10). Wang S et al. Genome profiling of mismatch repair genes in eight types of tumors. CELL CYCLE 2021 May 09; (PubMed: 33966609) [IF=4.3]
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