NCSTN Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from MCF7, using NCSTN Antibody.

Product:	NCSTN Antibody
Catalog:	DF6242
Description:	Rabbit polyclonal antibody to NCSTN
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	78kDa; 78kD(Calculated).
Uniprot:	Q92542
RRID:	AB_2838208

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(100%), Dog(83%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

NCSTN Antibody detects endogenous levels of total NCSTN.

RRID:

AB_2838208
Cite Format: Affinity Biosciences Cat# DF6242, RRID:AB_2838208.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Anterior pharynx defective 2; APH 2; APH2; ATAG1874; KIAA0253; Ncstn; NCT; NICA_HUMAN; Nicastrin; RP11 517F10.1; RP11517F101;

Immunogens

Immunogen:

Uniprot:

Q92542(NICA_HUMAN) >>Visit HPA database.

Gene(ID):

NCSTN(23385)

Expression:

Q92542 NICA_HUMAN:

Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676).

Description:

Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).

Sequence:

Q92542 NICA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92542 As Substrate

Q92542

Site	PTM Type	Enzyme	Source
N45	N-Glycosylation		Uniprot
N55	N-Glycosylation		Uniprot
K101	Ubiquitination		Uniprot
K110	Acetylation		Uniprot
K110	Ubiquitination		Uniprot
K112	Acetylation		Uniprot
K127	Ubiquitination		Uniprot
N187	N-Glycosylation		Uniprot
N264	N-Glycosylation		Uniprot
K349	Acetylation		Uniprot
K351	Ubiquitination		Uniprot
N387	N-Glycosylation		Uniprot
K403	Ubiquitination		Uniprot
S425	Phosphorylation		Uniprot
S426	Phosphorylation		Uniprot
R434	Methylation		Uniprot
N435	N-Glycosylation		Uniprot
S437	Phosphorylation	O00141 (SGK1)	Uniprot
N464	N-Glycosylation		Uniprot
N506	N-Glycosylation		Uniprot
N530	N-Glycosylation		Uniprot
N562	N-Glycosylation		Uniprot
N573	N-Glycosylation		Uniprot
N580	N-Glycosylation		Uniprot
N612	N-Glycosylation		Uniprot
S632	Phosphorylation		Uniprot
S638	Phosphorylation		Uniprot
T647	Phosphorylation		Uniprot
T649	Phosphorylation		Uniprot
T672	Phosphorylation		Uniprot
T674	Phosphorylation		Uniprot
S708	Phosphorylation		Uniprot

Research Backgrounds

Q92542_NICA_HUMAN

Function:

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

PTMs:

N-glycosylated.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:

Detected in brain (at protein level). Widely expressed.

Subunit Structure:

Component of the gamma-secretase complex. The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Interacts with PSEN1 and PSEN2.

Family&Domains:

Belongs to the nicastrin family.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

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NCSTN Antibody - #DF6242