EDN1 Antibody - #DF6125

FIGURE 5 miR-200a-3p directly targets the 3′UTR of MAPK14 and regulates the expression levels of p38, c-jun, ET-1 and VEGFA. (A) The MAPK14 3′-UTR containing the wildtype or mutant miR-200a-3p binding sequence was inserted into downstream of the luciferase reporter vector. The mutated sequences are italicized. (B) The dual luciferase reporter assay revealed that the luciferase activity controlled by MAPK14 3′-UTR was inhibited by ectopic miR-200a-3p expression in 293T cells. (C) miR-200a-3p was highly expressed or knocked down in HUVEC by lipofectamine 2000 transfection. QRT-PCR analysis was performed to measure the expression levels of miR-200a-3p in HUVEC after treatment with miR-200a-3p mimic, mimic control or miR-200a-3p inhibitor, inhibitor control. (D) The mRNA levels of MAPK14, c-jun, ET-1 and VEGFA were determined by qRT-PCR in HUVEC transfected with miR-200a-3p mimic, mimic control or miR-200a-3p inhibitor, inhibitor control. (E) Western blot analysis of p38, phosphorylated p38, c-jun, phosphorylated c-Jun, ET-1 and VEGFA protein levels in HUVEC transfected with miR-200a-3p mimic, mimic control or miR-200a-3p inhibitor, inhibitor control. (F) Quantification of protein results in panel E. Data were expressed as mean ± SEM. Between group differences were assessed by the student’s t test, respectively. *p < 0.05, **p < 0.01, ns, not significant.