Caspase 10 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat using CASP10 Antibody.

Product:	Caspase 10 Antibody
Catalog:	DF6021
Description:	Rabbit polyclonal antibody to Caspase 10
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	59kDa; 59kD(Calculated).
Uniprot:	Q92851
RRID:	AB_2837996

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Caspase 10 Antibody detects endogenous levels of total Caspase 10.

RRID:

AB_2837996
Cite Format: Affinity Biosciences Cat# DF6021, RRID:AB_2837996.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALPS2; Apoptotic protease Mch-4; CASP 10; CASP-10; CASP10; CASPA_HUMAN; Caspase 10 apoptosis related cysteine peptidase; Caspase-10 subunit p12; FADD like ICE2; Fas associated death domain protein; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE 2; FLICE2; ICE like apoptotic protease 4; ICE-like apoptotic protease 4; Interleukin 1B converting enzyme 2; MCH 4;

Immunogens

Immunogen:

Uniprot:

Q92851(CASPA_HUMAN) >>Visit HPA database.

Gene(ID):

CASP10(843)

Expression:

Q92851 CASPA_HUMAN:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Description:

Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).

Sequence:

Q92851 CASPA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS

PTMs - Q92851 As Substrate

Q92851

Site	PTM Type	Source
K2	Methylation	Uniprot
K2	Ubiquitination	Uniprot
S3	Phosphorylation	Uniprot
Y9	Phosphorylation	Uniprot
K14	Ubiquitination	Uniprot
K17	Ubiquitination	Uniprot
K23	Ubiquitination	Uniprot
K90	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
K158	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
K175	Ubiquitination	Uniprot
K180	Ubiquitination	Uniprot
K192	Ubiquitination	Uniprot
T198	Phosphorylation	Uniprot
S207	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
K235	Ubiquitination	Uniprot
S250	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot
T263	Phosphorylation	Uniprot
T268	Phosphorylation	Uniprot
S269	Phosphorylation	Uniprot
K271	Ubiquitination	Uniprot
S293	Phosphorylation	Uniprot
K338	Ubiquitination	Uniprot

Research Backgrounds

Q92851_CASPA_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.

Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.

Isoform C is proteolytically inactive.

PTMs:

Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

Tissue Specificity:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Interacts with RFFL and RNF34; negatively regulate CASP10 through proteasomal degradation. Interacts with RIOK3.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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Caspase 10 Antibody - #DF6021