NSE Antibody - #AF5473
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| Product: | NSE Antibody |
| Catalog: | AF5473 |
| Description: | Rabbit polyclonal antibody to NSE |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 47 kDa; 47kD(Calculated). |
| Uniprot: | P09104 |
| RRID: | AB_2837955 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5473, RRID:AB_2837955.
Fold/Unfold
2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Eno 2; ENO2; ENOG; ENOG_HUMAN; Enolase 2 (gamma, neuronal); Enolase 2; Enolase 2 gamma neuronal; Enolase2; Epididymis secretory protein Li 279; Gamma enolase; Gamma-enolase; HEL S 279; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; Neurone specific enolase; NSE;
Immunogens
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- P09104 ENOG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P09104 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K202 | Acetylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K228 | Acetylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y236 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K343 | Acetylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot |
Research Backgrounds
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cytoplasm. Cell membrane.
Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
Belongs to the enolase family.
Research Fields
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
References
Application: IF/ICC Species: mouse Sample: cortical neurons.
Application: WB Species: Rat Sample: brain tissue
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