Transferrin Receptor Antibody - #AF5343
Product: | Transferrin Receptor Antibody |
Catalog: | AF5343 |
Description: | Rabbit polyclonal antibody to Transferrin Receptor |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Monkey |
Prediction: | Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
Mol.Wt.: | 50kD(reduced),80~130kDa; 85kD(Calculated). |
Uniprot: | P02786 |
RRID: | AB_2837828 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5343, RRID:AB_2837828.
Fold/Unfold
CD 71; CD71; CD71 antigen; IMD46; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; p90; sTfR; T9; TFR 1; TfR; TfR1; TFR1_HUMAN; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin receptor protein 1, serum form; Trfr;
Immunogens
- P02786 TFR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02786 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S7 | Phosphorylation | Uniprot | |
S10 | Phosphorylation | Uniprot | |
S19 | Phosphorylation | Uniprot | |
Y20 | Phosphorylation | P12931 (SRC) | Uniprot |
T21 | Phosphorylation | Uniprot | |
S24 | Phosphorylation | P17252 (PRKCA) | Uniprot |
S34 | Phosphorylation | Uniprot | |
K39 | Ubiquitination | Uniprot | |
T52 | Phosphorylation | Uniprot | |
K53 | Acetylation | Uniprot | |
K53 | Ubiquitination | Uniprot | |
K58 | Ubiquitination | Uniprot | |
K60 | Ubiquitination | Uniprot | |
T104 | O-Glycosylation | Uniprot | |
S106 | Phosphorylation | Uniprot | |
Y123 | Phosphorylation | Uniprot | |
K128 | Acetylation | Uniprot | |
K128 | Ubiquitination | Uniprot | |
K134 | Ubiquitination | Uniprot | |
K145 | Ubiquitination | Uniprot | |
K161 | Ubiquitination | Uniprot | |
Y168 | Phosphorylation | Uniprot | |
K177 | Ubiquitination | Uniprot | |
K189 | Ubiquitination | Uniprot | |
K193 | Ubiquitination | Uniprot | |
S195 | Phosphorylation | Uniprot | |
S199 | Phosphorylation | Uniprot | |
K205 | Ubiquitination | Uniprot | |
Y219 | Phosphorylation | Uniprot | |
K224 | Ubiquitination | Uniprot | |
K231 | Ubiquitination | Uniprot | |
K240 | Ubiquitination | Uniprot | |
K241 | Ubiquitination | Uniprot | |
N251 | N-Glycosylation | Uniprot | |
K261 | Ubiquitination | Uniprot | |
Y282 | Phosphorylation | Uniprot | |
T286 | Phosphorylation | Uniprot | |
N317 | N-Glycosylation | Uniprot | |
S326 | Phosphorylation | Uniprot | |
S327 | Phosphorylation | Uniprot | |
S338 | Phosphorylation | Uniprot | |
K344 | Ubiquitination | Uniprot | |
K358 | Ubiquitination | Uniprot | |
K371 | Ubiquitination | Uniprot | |
K374 | Ubiquitination | Uniprot | |
K382 | Ubiquitination | Uniprot | |
K385 | Ubiquitination | Uniprot | |
K394 | Acetylation | Uniprot | |
Y402 | Phosphorylation | Uniprot | |
K418 | Acetylation | Uniprot | |
K418 | Ubiquitination | Uniprot | |
S419 | Phosphorylation | Uniprot | |
Y481 | Phosphorylation | Uniprot | |
K486 | Ubiquitination | Uniprot | |
S499 | Phosphorylation | Uniprot | |
Y503 | Phosphorylation | Uniprot | |
K508 | Ubiquitination | Uniprot | |
K514 | Ubiquitination | Uniprot | |
Y523 | Phosphorylation | Uniprot | |
K531 | Ubiquitination | Uniprot | |
T572 | Phosphorylation | Uniprot | |
Y573 | Phosphorylation | Uniprot | |
K585 | Ubiquitination | Uniprot | |
Y611 | Phosphorylation | Uniprot | |
S616 | Phosphorylation | Uniprot | |
S620 | Phosphorylation | Uniprot | |
T658 | Phosphorylation | Uniprot | |
K665 | Ubiquitination | Uniprot | |
Y683 | Phosphorylation | Uniprot | |
S687 | Phosphorylation | Uniprot | |
S691 | Phosphorylation | Uniprot | |
K693 | Ubiquitination | Uniprot | |
K717 | Ubiquitination | Uniprot | |
K720 | Ubiquitination | Uniprot |
Research Backgrounds
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cell membrane>Single-pass type II membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Secreted.
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3; facilitates TFRC endocytosis in erythroid precursor cells.
(Microbial infection) Interacts with Guanarito, Junin and Machupo arenavirus glycoprotein complex.
Belongs to the peptidase M28 family. M28B subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
References
Application: WB Species: Mouse Sample: renal tissues
Application: IHC Species: Mouse Sample: renal tissues
Application: WB Species: Rat Sample: spinal cord
Application: WB Species: Mouse Sample: brain
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