Cyclin A1 Antibody | Affinity Biosciences

Product:	Cyclin A1 Antibody
Catalog:	AF5313
Description:	Rabbit polyclonal antibody to Cyclin A1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	52 kDa; 52kD(Calculated).
Uniprot:	P78396
RRID:	AB_2837798

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

Cyclin A1 Antibody detects endogenous levels of total Cyclin A1.

RRID:

AB_2837798
Cite Format: Affinity Biosciences Cat# AF5313, RRID:AB_2837798.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCN A1; CCNA 1; Ccna1; CCNA1_HUMAN; Cyclin-A1; CyclinA1; G2/mitotic specific cyclin A1; MGC132235; MGC159139;

Immunogens

Immunogen:

Uniprot:

P78396(CCNA1_HUMAN) >>Visit HPA database.

Gene(ID):

CCNA1(8900)

Expression:

P78396 CCNA1_HUMAN:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Description:

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.

Sequence:

P78396 CCNA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVFQQQPVESEAMHCSNPKSGVVLATVARGPDACQILTRAPLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDELEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEYAEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSVLRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQYLRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVPCLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78396 As Substrate

P78396

Site	PTM Type	Source
	Ubiquitination	Uniprot
T168	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot

Research Backgrounds

P78396_CCNA1_HUMAN

Function:

PTMs:

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Subcellular Location:

Nucleus.

Tissue Specificity:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Subunit Structure:

Interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Does not bind CDK4 and CDK5 (in vitro). The cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins. Found in a complex with CDK2, CABLES1 and CCNE1 (By similarity). Interacts with INCA1. Interacts with KLHDC9.

Family&Domains:

Belongs to the cyclin family. Cyclin AB subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

References

1). PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer.. Cancer Research, 2023 (PubMed: 37646571) [IF=11.2]

Application: WB Species: Human Sample:

FigureS1.CyclinA1andA2arenotsuitableashistologicalpredictorsof5-FUresponseinGCpatients.A–D,ProteinexpressionofCyclinA1andCyclinA2inCyclinA1(CyclinA1OE)orA2(CyclinA2OE)overexpressingandknockingdown(shRNA1andshRNA2)AGS,MKN-45,NCI-N87 andSNU-216cellsdetectedbyImmunoblotting.E–H,PercentageofcellsindifferentphasesinCyclinA1OEorCyclinA2OEAGS,MKN-45,NCI-N87andSNU-216cellsdetectedbyFCM(n=3).I–K,Sensitivityto5-FUinCyclinA1OE,CyclinA2OEMKN-45,NCI-N87andSNU-216cells(n=4).L-O,Percentageofcellsindifferentphasesinknockingdown(shCyclinA1andshCyclinA2)AGS,MKN-45,NCI-N87andSNU-216cellsdetectedbyFCM(n=3).P-R,Sensitivityto5-FUinshRNA1andshRNA2MKN-45,NCI-N87andSNU-216cells(n=4).Alldataarethemean±SD.One-wayANOVA(G–L),

2). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.8]

Application: WB Species: mouse Sample: testis

Fig. 5. |SiNPs affected the protein expressions of cell cycle regulators. (A) The protein expressions of cyclin A1, CDK2, cyclin E1, CDK1, and cyclin B1 were significantly decreased in the testis after exposure to SiNPs.

3). STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 27940362) [IF=3.1]

Application: WB Species: mouse Sample:

STAT3 knockdown blocked cell cycle procession at S phase in adipogenic stem cells and early differentiating cells. (A) Flow cytometric analysis of 3T3-L1 stem cells at 48 h after siRNA introduction. Expression of cell cycle-associated genes was measured at mRNA (B) and protein (C) levels at 48 h after siRNA introduction in 3T3-L1 stem cells. (D) Flow cytometric analysis of 3T3-L1 cells that were differentiated for 24 h, with differentiation induced at 72 h after STAT3 siRNA introduction. qRT-PCR analysis (E) and Western blot analyses (F) of cell cycle-associated genes after STAT3 siRNA introduction. Values are presented as means ± S.D. of three replicates. * P < 0.01, # P < 0.05 vs siGL3.

4). Acrylamide-induced meiotic arrest of spermatocytes in adolescent mice by triggering excessive DNA strand breaks: Potential therapeutic effects of resveratrol. Human & experimental toxicology, 2023 (PubMed: 37550604) [IF=2.8]

Application: WB Species: Mouse Sample:

Figure 2. Effects of AA on proportions of testicular cell populations and expression of meiotic proteins. (a) Representative fluorescent cell sorting images of flow cytometric analysis of testicular cells. (b) The percentage of 1C, 2C and 4C testicular cells by flow cytometric analysis. (c) Representative immunofluorescence images of SYCP3 staining in the testis. (d) and (e) The expression levels of meiotic regulating factors (SYCP3, CDK2 and Cyclin A1) in the testis and isolated spermatocytes, respectively. The data are expressed as the mean ± SD of three mice in each group for annotations B and D, and are expressed as the mean ± SE of three separate experiments with triplicate samples for annotation E. *p < 0.05, **p < 0.01, compared with the control group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Cyclin A1 Antibody - #AF5313