Bax Antibody | Affinity Biosciences

$Fig. 3 AOS inhibited H2O2-induced apoptosis in HUVECs. Cells were pretreated using 200 lg/mL AOS before a 24 h stimulation using 400 lM H2O2, after which apoptosis was assessed by flow cytometry (a) and Hoechst 33,342 staining (b). The mRNA (c) and protein (d) levels of Bax, Bcl-2, and caspase 3. Data are expressed as mean ± S.E.M. and are representative of three independent experiments. *P\0.05 vs. control. **P \0.01 vs. control. # P \0.05 vs. H2O2. ##P \0.01 vs. H2O2$

Fig.

Figure 4.

Fig.

Figure 2 miR-15b reduces EC cell viability and induces apoptosis.

Figure 6.

Figure 4.

Figure 6.

Figure 5 SERPINE1 knockdown attenuates the effect of TGF-β1 on hemin-induced neuronal damage.

Fig.

Figure 2.

Figure 8 DBD inhibited the growth of metastatic tumor through inducing cell apoptosis.

Figure 2 Effect of treatment with HP on apoptosis of Dami cells.

Fig.

Figure 4.

Fig.

Figure 2 Quercetin inhibited cell apoptosis in the pancreas of T2DM mice (A) Bands of c-Caspase3, Bax, and Bcl-2 by western blotting in the pancreas of mice.

Figure 2.

FIGURE 1 Tan I inhibits oxidative stress and oxidative stress–induced H9C2 cell apoptosis.

Figure 6 Effects of Rg1 on the expression levels of Bax, Bcl-2 and cleaved-caspase-3 in LPS-induced HT22 cells determined by western blotting.

FIGURE 7 Relative protein levels of ALDH-2/4HNE/Caspase-3/cleaved caspase-3/Bax/Bcl-2 in the prefrontal cortex of CMS rats.

Figure 1.

Figure 4:| Anti-inflammatory effects of PK11195 and Midazolam on LPS-activated BV-2 cells protected NSC34 neuronal cells from cytotoxicity and provided neuroprotective effect against the NSC34 neuronal cell apoptosis.

FIGURE 2 Overexpression of miR27a in nucleus pulposus cells inhibits apoptosis and the production of inflammatory cytokines.

Fig.

Figure 4 B3GALT5-AS1/CSNK2A1 axis regulates cell apoptosis.

Figure 1 Chrysin inhibits the proliferation and induces the apoptosis of endometrial cancer cells.

Figure 7 Effect of LEO on the mRNA expression and protein expression of Bax, Bcl2, and caspase3 in HUVECs: (a) effect of LEO on the mRNA expression of Bax, Bcl2, and caspase3 in HUVECs; (b) effect of LEO on the protein expression of Bax, Bcl2, and caspase3 in HUVECs.

Figure 5 Effects of H2 on the TBHP-induced expression of cytokines and apoptosis-related proteins in human chondrocytes.

FIGURE 5 The aFGF–NP + CPMBs combined with UTMD inhibited cardiomyocyte apoptosis in DOX–HF rats.

Figure 5 Effect of miR-29b on the p53-mediated apoptotic pathway in H22 cells.

Fig.

Figure 4 miR-590-3p inhibits autophagy- and apoptosis-associated protein expression levels in H/R-treated cardiomyocytes.

Figure 5.

Figure 4.

Figure 2 Quercetin inhibited cell apoptosis in the pancreas of T2DM mice (A) Bands of c-Caspase3, Bax, and Bcl-2 by western blotting in the pancreas of mice.

FIGURE 4| Effect of P.

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

Figure 8 | C-PC inhibits apoptosis in lung tissue and MLE-12 cells.

Figure S1.

Figure 3 The influences of KRT80 on proliferation and cell cycle in ovarian cancer cells.

Figure 3 Effect of miR-128 knockdown on cardiomyocytes apoptosis induced by Ang II.

Figure 3 Effect of MAF on apoptosis induced by H/R.

Fig.

Figure 3:| Effect of miR-128 knockdown on cardiomyocytes apoptosis induced by Ang II.

Fig.

Figure 8 FA promoted the apoptosis of LX2 cells by regulating Bax/Bcl2 pathway.

Fig.

Figure 3 Effects of different doses of FJD on the Bcl-2 family protein (a) and the PI3K/AKT/mTOR/NF-κB (b) signaling pathway of protein levels in tumor tissues.

Figure 5 Mitochondrial transfer inhibits apoptosis in NPCs with mitochondrial dysfunction.

Figure 4.

Fig.

Figure 2 AQP5 deficiency induced ultrastructural abnormalities and endoplasmic reticulum (ER) stress in the lacrimal gland.

FIGURE 5 Expression and co-localization of Bax and Bcl2 in the rat dorsal CA1 region of hippocampus.

Figure 3 Expression levels of mTOR pathway proteins, ERS-associated proteins, and apoptosis-associated proteins in WT, CLP, and CLP+4-PBA mouse groups.

Figure 4 Puerarin treatment activates cell apoptosis and related proteins, while suppressing IL-6, TNF-α, and CA125 levels and modulating CD3+CD4+CD3+/CD8+ T lymphocyte percentages in OC tumor-bearing Fischer 344 rats.

Figure 3 SHMT2 knockdown activates caspase dependent apoptotic pathway.

Figure 6 RAC induced the apoptosis of the tumor tissue in RCC mice.

Fig.

Figure 4 Effect of LBAG on cardiomyocyte apoptosis in rats.

Figure 4.

Figure 7: |OLA inhibits autophagic flux and induces apoptosis.

Figure 4.

Fig.

Figure 4 Effects of SZY-200 on cell cycle progression and apoptosis rate in UM-UC-3 cells and scratch tests.

FIGURE 2 FG‐4592 inhibits apoptosis and promotes autophagy in bone marrow stromal cell (BMSC) post‐oxygen–glucose deprivation (OGD).

Figure 7 Treatment with C20/C22 induced ER stress and mitochondrial dysfunction increased the expression of proapoptotic proteins.

Figure 2 EA upregulated the expression of miR-206-3p and inhibited the expression of the target gene BDNF, while decreasing the expression of BAX/Bcl-2, IL-6, and TNF-α after CCI.

Figure 7 Effect of JXD on the cell apoptosis in pulmonary tissue of BLM-induced PF rats.

Figure 5.

Fig.

Figure 3 The effect of tumor-derived exosomes on pancreatic cancer (PC) on the xenograft tumor model.

Figure 4 Immunohistochemical staining of TLR4 (a), NF-κB (b), Bax (c), and Bcl-2 (d) and semiquantitative analysis of these proteins expression (e) in intestinal tissues of mice.

Figure 9 APL extract inhibited cardiomyocyte apoptosis of Iso-induced mice.

FIGURE 2 Licoricidin promoted MGC-803 cell apoptosis and arrested mitosis in the G0/G1 phase.

Figure 4 Effect of GA on the mitochondrial pathway of H9C2 cells.

Figure 4 Nrf2 deficiency promotes CLP-induced M1 macrophage polarization and apoptosis in lung tissues.

Fig.

FIGURE 9 TFA Reduces Iopromide induced Renal Tubular Cell Injury and Apoptosis by Regulating the Phosphorylation of AKT.

Figure 4.

Figure 1 ADR induces podocyte apoptosis and the regulation of miR-199b-5p.

Figure 4 MT decreased the rate of apoptosis in MC3T3-E1 cells that were iron-overloaded.

Fig.

FIGURE 2 GDF11 inhibited apoptosis of DGR in vitro.

Figure 5 Effect of CL and dark on the expression of histidine triad nucleotide binding protein 1 (HINT1), BAX and BCL-2 in SCN, PFC, NAc and Hip.

Figure 2.

Fig.

Figure 3 PARP14 deficiency exacerbates SCI-induced neuronal apoptosis at 7 days post-SCI.

Figure 3 ｜ Lycium barbarum polysaccharide combined with oxaliplatin inhibited the expression of PMI, ABCG2 and Bcl-2 and promoted the expression of Bax.

Fig.

FIGURE 3 Urolithin A reduces myocardial apoptosis.

Figure 7 Osthole decreases neuron loss and neuron apoptosis induced by D-galactose.

Figure 4 Minocycline suppresses the hippocampal apoptosis induced by sevoflurane: (A) TUNEL detection of neuronal apoptosis.

Figure 10 Protein levels of the Bcl-2 and BAX and Bcl-2/BAX ratios in bEECs.

Fig.

Figure 3 High-dose VC suppressed the cardiomyocyte apoptosis in CLP-induced sepsis rats at 1d, 3d and 5d.

Figure 7 BA inhibits apoptosis and oxidative stress and promotes autophagy in the spinal cord tissue of SCI rats.

FIGURE 5 Effects of Ginsenoside Rb1 and Ginsenoside CK on Apoptosis-related Proteins in HGC-27 (A) Apoptosis-related proteins Bax, Bcl-2, Caspase 3 bands.

Product:	Bax Antibody
Catalog:	AF0120
Description:	Rabbit polyclonal antibody to Bax
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	21kDa; 21kD(Calculated).
Uniprot:	Q07812
RRID:	AB_2833304

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MSDS

Data Sheet

COA

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Bax Antibody detects endogenous levels of total Bax.

RRID:

AB_2833304
Cite Format: Affinity Biosciences Cat# AF0120, RRID:AB_2833304.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apoptosis regulator BAX; BAX; Bax-protein; BAX_HUMAN; BAXA; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bcl-2-like protein 4; BCL2 associated X protein; BCL2 associated X protein omega; BCL2 associated X protein transcript variant delta2; Bcl2-L-4; BCL2L4; membrane isoform alpha;

Immunogens

Immunogen:

Uniprot:

Q07812(BAX_HUMAN) >>Visit HPA database.

Gene(ID):

BAX(581)

Expression:

Q07812 BAX_HUMAN:

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Description:

Bax Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis. Belongs to the Bcl-2 family. Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). 8 isoforms of the human protein are produced by alternative splicing.

Sequence:

Q07812 BAX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07812 As Substrate

Q07812

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
R9	Methylation		Uniprot
S15	Phosphorylation		Uniprot
K21	Ubiquitination		Uniprot
T22	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
K58	Ubiquitination		Uniprot
S60	Phosphorylation		Uniprot
K64	Ubiquitination		Uniprot
T85	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
T135	Phosphorylation		Uniprot
T140	Phosphorylation		Uniprot
S163	Phosphorylation	P49841 (GSK3B)	Uniprot
Y164	Phosphorylation		Uniprot
T167	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T172	Phosphorylation		Uniprot
T174	Phosphorylation		Uniprot
S184	Phosphorylation	Q05513 (PRKCZ) , P31749 (AKT1)	Uniprot
T186	Phosphorylation		Uniprot

Research Backgrounds

Q07812_BAX_HUMAN

Function:

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein. Cytoplasm.
Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.

Cytoplasm.

Cytoplasm.

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Homodimer. Forms higher oligomers under stress conditions. Forms heterooligomers with BAK. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with RTL10/BOP. Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria. Interacts with GIMAP3/IAN4 and GIMAP5/IAN5; this interaction is increased, when cells initiate apoptosis upon IL2 withdrawal.

(Microbial infection) Interacts with adenovirus E1B 19K protein; this interaction blocks BAX oligomerization.

(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37.

(Microbial infection) Interacts with enterovirus protein 2B; this interaction activates BAX-induced apoptosis.

Family&Domains:

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Belongs to the Bcl-2 family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Glioma. (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

References

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=14.9]

Application: WB Species: human Sample: HepG2

Figure 4. Effect of A22 on anti-apoptosis in 0.5 mM palmitic acid oil (PA) induced cell model. (A) Effect of A22 on cell viability for anti-apoptotic protective effect. (B) Effect of A22 on transcription of BCL-2 and BAX with measurement of mRNA levels. (C) Effect of A22 on protein expressions related with apoptosis (left), which were quantitatively analyzed (right). All the experiments were repeated for three times.

2). Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice. JOURNAL OF HAZARDOUS MATERIALS, 2021 (PubMed: 33087287) [IF=13.6]

Application: WB Species: mice Sample: testis tissue

Fig. 9. Bax and Bcl2 protein expression in the testis tissue mice by western blotting. The statistical chart shows the rate of Bax to Bcl2, which takes into account the balance of apoptosis and anti- apoptosis in testis. *P < 0.05, compared with the corresponding control, , $P < 0.05 compared with the M-Dose group.

3). Oxygen vacancy-engineered cerium oxide mediated by copper-platinum exhibit enhanced SOD/CAT-mimicking activities to regulate the microenvironment for osteoarthritis therapy. Journal of nanobiotechnology, 2024 (PubMed: 39155382) [IF=10.2]

Application: IF/ICC Species: Rat Sample:

Fig. 5. ROS scavenging, mitochondrial protection, and apoptosis inhibition by PtCuOX/CeO2-X nanozymes in vitro. a Chondrocyte staining with various ROS assay kits and b corresponding quantification to assess the scavenging capacity of ROS, ·O2−, ·OH, and NO, respectively. c Chondrocyte staining with mitochondrial membrane potential probe (JC-1) and d corresponding quantification to assess the membrane potential. e Chondrocyte staining with MitoSOX probe and f corresponding quantification to assess the level of endogenous ROS. g Chondrocyte staining with Fluo-4 AM probe and h corresponding quantification to assess the disturbance of Ca2+ efflux. i) ATP assay kit to assess ATP levels in chondrocytes after different treatments. j 7-AAD/Annexin V-APC apoptosis kit to assess apoptosis in chondrocytes after different treatments. k Western blotting and l quantitative analysis to assess Bax, Caspase-3, and Bcl-2 protein levels. Concentration: 50 μg/mL; NIR parameters: 808 nm, 1.0 W/cm2, 5 min. Data are expressed as mean ± SD (n = 3). * and # for P

4). Biomimetic Nano-Regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma. Advanced healthcare materials, 2024 (PubMed: 38855966) [IF=10.0]

5). Smart dual responsive nanocarriers with reactive oxygen species amplification assisted synergistic chemotherapy against prostate cancer. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2022 (PubMed: 35544966) [IF=9.9]

6). Urolithin A protects severe acute pancreatitis-associated acute cardiac injury by regulating mitochondrial fatty acid oxidative metabolism in cardiomyocytes. MedComm, 2023 (PubMed: 38116065) [IF=9.9]

Application: WB Species: Mouse Sample: cardiac tissues

FIGURE 3 Urolithin A reduces myocardial apoptosis. (A) Representative TUNEL staining images of heart tissues in the sham, SACI and UA groups (scale bar = 100 μm; n = 3). (B) Western blotting and quantitative analyses of Bcl‐2, Bax, Cleaved‐Caspase3 in cardiac tissues of the sham, SACI, and UA groups (n = 3). (C) Bcl‐2 and Cleaved‐Caspase3 expression in the heart tissues of mice as detected by immunohistochemistry (scale bar = 50 μm; n = 6). The results are presented as the mean ± SD. **p < 0.01, ***p < 0.005, ****p < 0.0001 vs. sham group; #p < 0.05, ##p < 0.01, ###p < 0.005, ####p < 0.0001 vs. SACI group, by one‐way ANOVA tests followed by Tukey tests.

7). Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection. Acta Biomaterialia, 2022 (PubMed: 34902617) [IF=9.7]

8). Engineering Chemotherapeutic-Augmented Calcium Phosphate Nanoparticles for Treatment of Intraperitoneal Disseminated Ovarian Cancer. ACS Applied Materials & Interfaces, 2022 (PubMed: 35508299) [IF=9.5]

9). A marine-derived small molecule induces immunogenic cell death against triple-negative breast cancer through ER stress-CHOP pathway. International Journal of Biological Sciences, 2023 (PubMed: 35541893) [IF=9.2]

Application: WB Species: mouse Sample:

Figure S1. |Antitumor effect of MHO7 in vitro and in vivo.(C-D) The expression of Bxl2-2, Bax and cleaved caspase 3 were measured by western blot after the treatment of MHO7.

10). LNC473 regulating APAF1 IRES-dependent translation via competitive sponging miR574 and miR15b: Implications in colorectal cancer. Molecular Therapy-Nucleic Acids, 2020 (PubMed: 32784109) [IF=8.8]

Application: WB Species: Human Sample: HCT116 and SW480 cells

Figure 6. LNC473-miR574/miR15b-APAF1 Signaling Axis in HCT116 and SW480 Cells (A) IF and ISH combination assay revealing the co-localization of APAF1 protein with included ncRNAs in HCT116 cells. Scale bars, 5 mm. (B and C) The levels of APAF1 mRNA (B) and protein (C) were determined after interfering LNC473 expression in HCT116 and SW480 cells by qPCR and IF assays. Scale bars, 20 mm. (D) The expression of apoptosis-related proteins including APAF1 was detected after interfering LNC473 expression in HCT116 and SW480 cells by western blot assay. (E and F) Rescue experiments showing the APAF1 levels in HCT116 and SW480 cells with exposure to the co-transfection of pcDH-LNC473 vector and miR574-5p or miR15b-5p mimic by qPCR (E) and western blot (F) assays. (G) Pattern diagram of APAF1-CDS and APAF1-IRES-CDS vectors. (H and I) APAF1 protein expression was determined in HCT116 and SW480 cells treated with APAF1-IRES-CDS vector, or pcDH-LNC473 and APAF1-IRES-CD co-transfection by western blot (H) and IF assays (I). Scale bars, 5 mm. (J) The percentage (%) of cell apoptosis in cells upon co-overexpressing APAF1-CDS or APAF1-IRE-CDS and LNC473 as assayed by flow cytometry. All tests were performed at least three times. Data were expressed as mean ± SD. ns (nonsignificant), p > 0.05; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Bax Antibody - #AF0120

Related products

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Immunogens

Predictions

PTMs - Q07812 As Substrate

Research Backgrounds

Research Fields

References

Restrictive clause