EPO Antibody - #AF5190

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Product: EPO Antibody
Catalog: AF5190
Description: Rabbit polyclonal antibody to EPO
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 21 kDa; 21kD(Calculated).
Uniprot: P01588
RRID: AB_2837676

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(90%), Dog(80%)
Clonality:
Polyclonal
Specificity:
EPO Antibody detects endogenous levels of total EPO.
RRID:
AB_2837676
Cite Format: Affinity Biosciences Cat# AF5190, RRID:AB_2837676.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EP; EPO alpha; EPO; Epoetin; Erythropoetin; Erythropoietin precursor; MVCD2;

Immunogens

Immunogen:
Uniprot:

P01588(EPO_HUMAN) >>Visit HPA database.

Gene(ID):
EPO(2056)
Expression:
P01588 EPO_HUMAN:

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Description:
Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence:
MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Xenopus
67
Chicken
50
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01588 As Substrate

Site PTM Type Enzyme
S111 Phosphorylation
S127 Phosphorylation
S131 Phosphorylation
S153 O-Glycosylation
T161 Phosphorylation

Research Backgrounds

Function:

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Family&Domains:

Belongs to the EPO/TPO family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

References

1). Case Report: Myomatous erythrocytosis syndrome presenting as rapid growth of an extra-uterine mass. Frontiers in Surgery, 2022 (PubMed: 35983553) [IF=1.8]

Application: IHC    Species: Human    Sample: myomatous tissue

Figure 2 (A–D): Immunohistochemical staining revealed excessive Epo and Epo-R expression of the broad ligament myoma. Immunochemical staining showed diffuse (>50%) Epo expression with moderate to strong staining intensity over the myomatous cells (A, 40×), whereas the endothelial cells were negatively stained (B, 200×). A diffuse (>50%) Epo-R expression with moderate staining intensity was found over the myomatous cells (C, 40×). The endothelial cells also presented with diffuse, moderate to weak staining intensity (D, 200×). EPO, erythropoietin; Epo-R, erythropoietin receptor.

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