IL4 Antibody - #AF5142

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Product: IL4 Antibody
Catalog: AF5142
Description: Rabbit polyclonal antibody to IL4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit
Mol.Wt.: 17kD,30kD; 17kD(Calculated).
Uniprot: P05112
RRID: AB_2837628

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(86%), Rabbit(86%)
Clonality:
Polyclonal
Specificity:
IL4 Antibody detects endogenous levels of total IL4.
RRID:
AB_2837628
Cite Format: Affinity Biosciences Cat# AF5142, RRID:AB_2837628.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; B-cell stimulatory factor 1; BCGF 1; BCGF1; Binetrakin; BSF-1; BSF1; IGG1 induction factor; IL 4; IL-4; IL4; IL4_HUMAN; Il4e12; Interleukin 4; Interleukin 4 variant 2; Interleukin 4, isoform 1; Interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra;

Immunogens

Immunogen:
Uniprot:

P05112(IL4_HUMAN) >>Visit HPA database.

Gene(ID):
IL4(3565)
Description:
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Sequence:
MGLTSQLLPPLFFLLACAGNFVHGHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
86
Rabbit
86
Dog
71
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05112 As Substrate

Site PTM Type Enzyme
N62 N-Glycosylation
T68 Phosphorylation

Research Backgrounds

Function:

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the IL-4/IL-13 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Asthma.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Allograft rejection.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

References

1). Functionalized injectable hyaluronic acid hydrogel with antioxidative and photothermal antibacterial activity for infected wound healing. International Journal of Biological Macromolecules, 2022 (PubMed: 35537589) [IF=8.2]
2). Role of transient receptor potential ankyrin 1 in idiopathic pulmonary fibrosis: modulation of M2 macrophage polarization. Cellular and molecular life sciences : CMLS, 2024 (PubMed: 38635081) [IF=8.0]

Application: WB    Species: Mouse    Sample:

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 

Application: IHC    Species: Mouse    Sample:

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 
3). Murine Chronic Pancreatitis Model Induced by Partial Ligation of the Pancreatic Duct Encapsulates the Profile of Macrophage in Human Chronic Pancreatitis. Frontiers in Immunology, 2022 (PubMed: 35432336) [IF=7.3]

Application: IF/ICC    Species: mouse    Sample:

FIGURE 7 | The expression of IL-4 increased in human CP specimens, as well as in mice CP models as the disease progressed and peaked at 21d. A large number of a-SMA+IL-4+cells, which represents PSCs secreting IL-4, were found in the pancreas of mice CP models and human CP. However, almost no CD4+IL-4+cells in the pancreas of mice CP models and human CP were observed. (A, C) Immunofluorescence staining of pancreatic IL-4 co-localized with a-SMA or CD4 in mice model of pancreatitis at different time points.
4). Nocardia rubra cell-wall skeleton activates an immune response in cervical tissue via stimulating FPR3 to enhance dendritic cell-mediated Th1 differentiation. Frontiers in Immunology, 2023 (PubMed: 36936958) [IF=7.3]
5). Role of LECT2 in exacerbating atopic dermatitis: insight from in vivo and in vitro models via NF-κB signaling pathway. Frontiers in immunology, 2024 (PubMed: 39206203) [IF=7.3]

Application: IHC    Species: Mouse    Sample:

Figure 2. Effect of LECT2 on the expression of the barrier proteins (FLG, IVL, and LOR) and the inflammatory factors (IL-1β and IL-4) in DNCB-induced AD-like skin lesions. (A) The immunostaining for each protein was brown, with darker colors representing higher expression of the protein. (B) Western Blot analysis of the levels of barrier proteins (C) FLG, (D) IVL, and (E) LOR in DNCB-induced AD-like skin lesions. WT indicates wild-type mice, and KO indicates knockout mice. The data shown represent mean ± SEM and are representative of at least three independent experiments. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.
6). Oroxylin A inhibits carcinogen-induced skin tumorigenesis through inhibition of inflammation by regulating SHCBP1 in mice. International Immunopharmacology, 2020 (PubMed: 31927505) [IF=5.6]

Application: WB    Species: mouse    Sample: skin tumor and serum

Fig. 2. | Effect of oroxylin A on DMBA/TPA-induced inflammation in skin tumor and serum of mice. (A-D) The effects of oroxylin A on the levels of IL-1β, IL-6,IL-18 and TNF-α in skin tumor and blood were determined respectively (n = 3). (E) Protein expression of IL-1β, NLRP3, IL-6, IL-18 and TNF-α were measured by Western blot assays (n = 3). GAPDH was used as an invariant control for equal loading. Representative blots are shown with densitometry.

Application: IHC    Species: mouse    Sample: TPA-induced skin tumor

Fig. 2. | Effect of oroxylin A on DMBA/TPA-induced inflammation in skin tumor and serum of mice. (A-D) The effects of oroxylin A on the levels of IL-1β, IL-6,IL-18 and TNF-α in skin tumor and blood were determined respectively (n = 3). (E) Protein expression of IL-1β, NLRP3, IL-6, IL-18 and TNF-α were measured by Western blot assays (n = 3). GAPDH was used as an invariant control for equal loading. Representative blots are shown with densitometry.. (F) Quantitation of the result of western blot. (G) Immunohistochemical assay of protein expressions of IL-1β and IL-4 in TPA-induced skin tumor (n = 3).
7). Regulatory effects of miRNA-126 on Th cell differentiation and cytokine expression in allergic rhinitis. Cellular Signalling, 2022 (PubMed: 35953026) [IF=4.8]
8). Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection. Chinese Journal of Natural Medicines, 2019 (PubMed: 31526500) [IF=4.6]

Application: WB    Species: mouse    Sample: lung

Fig. 6| Effects of GGD on Th1/Th2 balance. mRNA expression of (a) IFN-γ and (b) IL-4 determined by RT-PCR on day 4 post-infection (n = 5). (c) Protein expression of IFN-γ and IL-4 in lung tissue determined by western blotting (n = 3).
9). Blood HDAC4 Variation Links With Disease Activity and Response to Tumor Necrosis Factor Inhibitor and Regulates CD4+ T Cell Differentiation in Ankylosing Spondylitis. Frontiers in Medicine, 2022 (PubMed: 35602496) [IF=3.9]
10). Decreased glycolysis at menstruation is associated with increased menstrual blood loss. Reproductive Sciences, 2022 (PubMed: 36042151) [IF=2.9]
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