Angiopoietin 2 Antibody - #AF5124

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Product: Angiopoietin 2 Antibody
Catalog: AF5124
Description: Rabbit polyclonal antibody to Angiopoietin 2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 55~70kDa; 57kD(Calculated).
Uniprot: O15123
RRID: AB_2837610

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%)
Clonality:
Polyclonal
Specificity:
Angiopoietin 2 Antibody detects endogenous levels of total Angiopoietin 2.
RRID:
AB_2837610
Cite Format: Affinity Biosciences Cat# AF5124, RRID:AB_2837610.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGPT 2; Agpt2; ANG 2; ANG-2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin-2; Angiopoietin2; ANGP2_HUMAN; ANGPT 2; Angpt2; Tie2 ligand;

Immunogens

Immunogen:
Uniprot:

O15123(ANGP2_HUMAN) >>Visit HPA database.

Gene(ID):
ANGPT2(285)
Description:
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression.
Sequence:
MWQIVFFTLSCDLVLAAAYNNFRKSMDSIGKKQYQVQHGSCSYTFLLPEMDNCRSSSSPYVSNAVQRDAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSAKDPTVAKEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
78
Dog
78
Rabbit
78
Bovine
53
Sheep
53
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15123 As Substrate

Site PTM Type Enzyme
S208 Phosphorylation
S242 Phosphorylation
Y482 Phosphorylation

Research Backgrounds

Function:

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TEK/TIE2, competing for the same binding site as ANGPT1.

Family&Domains:

The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

References

1). Bone marrow-derived mesenchymal stem cells transplantation attenuates renal fibrosis following acute kidney injury by repairing the peritubular capillaries. Experimental Cell Research, 2022 (PubMed: 34921827) [IF=3.7]
2). Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation. Biology of Reproduction, 2019 (PubMed: 30084973) [IF=3.6]
3). Distinct effects of ANGPT2 on gene expression of glomerular podocytes and mesangial cells. American Journal of Translational Research, 2021 (PubMed: 34956451) [IF=2.2]

Application: IF/ICC    Species: human and mouse    Sample: glomerular cells

Figure 2 ANGPT2 protein expression in glomerular cells of human and mouse. A. Immunofluorescence staining of ANGPT2 in normal human glomeruli. Podocyte marker, SYNPO, was co-stained, thereby localizing ANGPT2 to podocytes and non-podocytes. B. Similar co-staining of ANGPT2 and SYNPO in mouse glomeruli showing ANGPT2 is present in both podocytes and non-podocytes in mice.

Application: WB    Species: Human    Sample: mesangial cells

Figure 10 pAKT was increased in ANGPT2-treated podocytes but downregulated in mesangial cells in culture. Podocytes and mesangial cells were treated with 500 ng/ml ANGPT2 for 24 h and subject to immnunoblotting. The results represent the data from three independent experiments and are expressed as mean ± SD. **P<0.01 vs. control.
4). [Dose and timing of normal saline resuscitation on endothelial glycocalyx in early septic shock]. , 2018 (PubMed: 30045788)

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