IL5 Antibody - #AF5123

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Product: IL5 Antibody
Catalog: AF5123
Description: Rabbit polyclonal antibody to IL5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 15 kDa; 15kD(Calculated).
Uniprot: P05113
RRID: AB_2837609

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(89%)
Clonality:
Polyclonal
Specificity:
IL5 Antibody detects endogenous levels of total IL5.
RRID:
AB_2837609
Cite Format: Affinity Biosciences Cat# AF5123, RRID:AB_2837609.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; IL-5; IL5; IL5_HUMAN; Interleukin 5 (colony stimulating factor, eosinophil); Interleukin 5; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; TRF;

Immunogens

Immunogen:
Uniprot:

P05113(IL5_HUMAN) >>Visit HPA database.

Gene(ID):
IL5(3567)
Description:
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Sequence:
MRMLLHLSLLALGAAYVYAIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Pig
78
Horse
78
Bovine
78
Sheep
78
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05113 As Substrate

Site PTM Type Enzyme
T22 O-Glycosylation
N47 N-Glycosylation
T73 Phosphorylation

Research Backgrounds

Function:

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the IL-5 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Asthma.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Allograft rejection.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

References

1). Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheep. Frontiers in Immunology, 2023 (PubMed: 37275885) [IF=7.3]

Application: WB    Species: Hu Sheep    Sample: liver tissue

Figure 8 Effect of DF supplementation on mitophagy. (A) Expression of autophagy-related genes. (B, C) Expression of autophagy-related proteins in the whole cell lysate. (D, E) Expression of mitophagy-related proteins in the mitochondria protein extract. n=6 per group and the values are the mean ± SEM. ** P
2). Pongamol Alleviates Neuroinflammation and Promotes Autophagy in Alzheimer's Disease by Regulating the Akt/mTOR Signaling Pathway. Journal of agricultural and food chemistry, 2024 (PubMed: 38841893) [IF=6.1]
3). Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet. Journal of Dairy Science, 2023 (PubMed: 36631320) [IF=3.5]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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