CREB3L2 Antibody - #DF4662

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Product: CREB3L2 Antibody
Catalog: DF4662
Description: Rabbit polyclonal antibody to CREB3L2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58 KD; 57kD(Calculated).
Uniprot: Q70SY1
RRID: AB_2837013

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CREB3L2 Antibody detects endogenous levels of total CREB3L2.
RRID:
AB_2837013
Cite Format: Affinity Biosciences Cat# DF4662, RRID:AB_2837013.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B ZIB transcription factor; Basic transcription factor 2; BBF2 human homolog on chromosome 7; BBF2H7; BZIB transcription factor; cAMP responsive element binding protein 3 like 2; cAMP-responsive element-binding protein 3-like protein 2; CR3L2_HUMAN; Creb3l2; Cyclic AMP-responsive element-binding protein 3-like protein 2; FUS/BBF2H7 protein; MGC131709; MGC71006; Processed cyclic AMP-responsive element-binding protein 3-like protein 2; TCAG_1951439;

Immunogens

Immunogen:
Uniprot:

Q70SY1(CR3L2_HUMAN) >>Visit HPA database.

Gene(ID):
CREB3L2(64764)
Expression:
Q70SY1 CR3L2_HUMAN:

Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.

Sequence:
MEVLESGEQGVLQWDRKLSELSEPGDGEALMYHTHFSELLDEFSQNVLGQLLNDPFLSEKSVSMEVEPSPTSPAPLIQAEHSYSLCEEPRAQSPFTHITTSDSFNDDEVESEKWYLSTDFPSTSIKTEPVTDEPPPGLVPSVTLTITAISTPLEKEEPPLEMNTGVDSSCQTIIPKIKLEPHEVDQFLNFSPKEAPVDHLHLPPTPPSSHGSDSEGSLSPNPRLHPFSLPQTHSPSRAAPRAPSALSSSPLLTAPHKLQGSGPLVLTEEEKRTLIAEGYPIPTKLPLSKSEEKALKKIRRKIKNKISAQESRRKKKEYMDSLEKKVESCSTENLELRKKVEVLENTNRTLLQQLQKLQTLVMGKVSRTCKLAGTQTGTCLMVVVLCFAVAFGSFFQGYGPYPSATKMALPSQHSLQEPYTASVVRSRNLLIYEEHSPPEESSSPGSAGELGGWDRGSSLLRVSGLESRPDVDLPHFIISNETSLEKSVLLELQQHLVSAKLEGNETLKVVELDRRVNTTF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Horse
82
Rabbit
82
Chicken
63
Xenopus
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q70SY1 As Substrate

Site PTM Type Enzyme
S72 Phosphorylation
S93 Phosphorylation
K176 Ubiquitination
K178 Sumoylation
K178 Ubiquitination
S191 Phosphorylation
S228 Phosphorylation
T232 Phosphorylation
S234 Phosphorylation
S244 Phosphorylation
S249 Phosphorylation
K257 Ubiquitination
S261 Phosphorylation
T267 Phosphorylation
K271 Ubiquitination
K289 Ubiquitination
S290 Phosphorylation
K305 Sumoylation
K305 Ubiquitination
K325 Sumoylation
K325 Ubiquitination
K339 Ubiquitination
K356 Ubiquitination
K364 Ubiquitination
T368 Phosphorylation
T482 Phosphorylation

Research Backgrounds

Function:

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes (By similarity). In a neuroblastoma cell line, protects cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site.

PTMs:

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).

N-glycosylated.

Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.
Note: ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.

Nucleus.
Note: Upon ER stress, translocated into the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.

Subunit Structure:

Binds DNA as a dimer.

Family&Domains:

Belongs to the bZIP family. ATF subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

References

1). miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes. Cell stress & chaperones, 2024 (PubMed: 38582327) [IF=3.8]

Application: WB    Species: Rat    Sample: H9c2 cardiomyocytes

Fig. 3 miR-92a-3p involved in the regulation of ethanol-induced apoptosis of H9c2 cardiomyocytes by targeting MSK2 and CREB3L2. (a) Prediction of miR-92a-3p binding sites; (b) validation of the target genes using RT-qPCR; (c) Western blot of MSK2 and CREB3L2 expression upon miR-92a-3p mimic or inhibitor transfection in the ethanol-induced apoptosis of H9c2 cardiomyocytes (n = 3; *P 

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