DVL2 Antibody - #DF4454

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Product: DVL2 Antibody
Catalog: DF4454
Description: Rabbit polyclonal antibody to DVL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90 KD; 79kD(Calculated).
Uniprot: O14641
RRID: AB_2836809

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
DVL2 Antibody detects endogenous levels of total DVL2.
RRID:
AB_2836809
Cite Format: Affinity Biosciences Cat# DF4454, RRID:AB_2836809.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dishevelled 2 (homologous to Drosophila dsh); Dishevelled dsh homolog 2; dishevelled segment polarity protein 2; Dishevelled-2; Dishevelled2; DSH homolog 2; DVL 2; Dvl2; DVL2_HUMAN; Segment polarity protein dishevelled homolog DVL 2; Segment polarity protein dishevelled homolog DVL-2; Segment polarity protein dishevelled homolog DVL2;

Immunogens

Immunogen:
Uniprot:

O14641(DVL2_HUMAN) >>Visit HPA database.

Gene(ID):
DVL2(1856)
Sequence:
MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
89
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14641 As Substrate

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T14 Phosphorylation
Y18 Phosphorylation P12931 (SRC)
Y27 Phosphorylation P12931 (SRC)
K30 Ubiquitination
T39 Phosphorylation
K44 Ubiquitination
K54 Ubiquitination
K58 Ubiquitination
S59 Phosphorylation
S135 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation P49674 (CSNK1E) , P48730 (CSNK1D)
T151 Phosphorylation
T153 Phosphorylation
S155 Phosphorylation
S158 Phosphorylation
S169 Phosphorylation
S170 Phosphorylation
S195 Phosphorylation
S196 Phosphorylation
T206 Phosphorylation P53350 (PLK1) , PR:P53350 (hPLK1)
S211 Phosphorylation
S221 Phosphorylation
S222 Phosphorylation
T224 Phosphorylation P48730 (CSNK1D) , P49674 (CSNK1E)
S227 Phosphorylation
S228 Phosphorylation
S230 Phosphorylation
T250 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
S255 Phosphorylation
T257 Phosphorylation
Y275 Phosphorylation P12931 (SRC)
S286 Phosphorylation
Y295 Phosphorylation P12931 (SRC)
K301 Ubiquitination
K343 Ubiquitination
K353 Ubiquitination
S358 Phosphorylation
Y362 Phosphorylation
T364 Phosphorylation
S418 Phosphorylation
K428 Ubiquitination
Y463 Phosphorylation P12931 (SRC)
K477 Ubiquitination
Y478 Phosphorylation
K484 Ubiquitination
S591 Phosphorylation
S592 Phosphorylation
S594 Phosphorylation
T595 Phosphorylation
S597 Phosphorylation
S614 Phosphorylation
K615 Ubiquitination
S626 Phosphorylation
R627 Methylation
S630 Phosphorylation
R633 Methylation
R649 Methylation
S651 Phosphorylation
R659 Methylation
T715 Phosphorylation
S720 Phosphorylation
S729 Phosphorylation

Research Backgrounds

Function:

Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.

PTMs:

Phosphorylated by CSNK1D. WNT3A induces DVL2 phosphorylation by CSNK1E and MARK kinases.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytosol. Cytoplasmic vesicle. Nucleus.
Note: Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta (By similarity). Interaction with FOXK1 and FOXK2 induces nuclear translocation (PubMed:25805136).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2. Interacts with Rac. Interacts with ARRB1; the interaction is enhanced by phosphorylation of DVL1 (By similarity). Can form large oligomers (via DIX domain). Interacts (via DIX domain) with DIXDC1 (via DIX domain). Interacts (via DEP domain) with AP2M1 and the AP-2 complex (By similarity). Interacts with DACT1 and FAM105B/otulin. Interacts with DCDC2. Interacts (when phosphorylated) with FOXK1 and FOXK2; the interaction induces DVL2 nuclear translocation. Interacts with MAPK15 (By similarity). Interacts with PKD1 (via extracellular domain). Interacts with LMBR1L (By similarity).

Family&Domains:

The DIX domain mediates homooligomerization.

Belongs to the DSH family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. Journal of Translational Medicine, 2021 (PubMed: 34001134) [IF=7.4]

Application: WB    Species: human    Sample:

Fig. 7| High expression of PRICKEL1 accompanied with core PCP pathway components and the migration and invasion components up-regulation in AML patients. a The amounts of the indicated PCP proteins were analyzed by Western blotting in AML patients (n=5) and normal controls(n=3).

Application: WB    Species: Human    Sample:

Fig. 7 High expression of PRICKEL1 accompanied with core PCP pathway components and the migration and invasion components up-regulation in AML patients. a The amounts of the indicated PCP proteins were analyzed by Western blotting in AML patients (n = 5) and normal controls (n = 3). b Densitometric quantification of data in a, the expression of the PCP proteins DVL2, PRICKLE1, LEF1 and active β-catenin were analyzed. c RNA-Seq analysis of gene expression in AML patients. Hierarchical cluster analysis of DEGs in AML patients (n = 4) and normal controls (n = 3), migration and invasion related genes were shown

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