PDHK1 Antibody - #DF4365

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Product: PDHK1 Antibody
Catalog: DF4365
Description: Rabbit polyclonal antibody to PDHK1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 49 KD; 49kD(Calculated).
Uniprot: Q15118
RRID: AB_2836733

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Dog(82%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
PDHK1 Antibody detects endogenous levels of total PDHK1.
RRID:
AB_2836733
Cite Format: Affinity Biosciences Cat# DF4365, RRID:AB_2836733.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; PDK1_HUMAN; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1;

Immunogens

Immunogen:
Uniprot:

Q15118(PDK1_HUMAN) >>Visit HPA database.

Gene(ID):
PDK1(5163)
Expression:
Q15118 PDK1_HUMAN:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

Sequence:
MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
91
Sheep
91
Dog
82
Chicken
80
Xenopus
78
Zebrafish
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15118 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
S67 Phosphorylation
K133 Ubiquitination
Y136 Phosphorylation P11362 (FGFR1)
K230 Ubiquitination
Y243 Phosphorylation P11362 (FGFR1) , O60674 (JAK2) , P00519 (ABL1) , P36888 (FLT3)
Y244 Phosphorylation P11362 (FGFR1)
R319 Methylation
Y336 Phosphorylation
T338 Phosphorylation P00558 (PGK1)
T346 Phosphorylation
S347 Phosphorylation
Y357 Phosphorylation
S393 Phosphorylation
S396 Phosphorylation
Y403 Phosphorylation
K405 Ubiquitination

PTMs - Q15118 As Enzyme

Substrate Site Source
P05106-1 (ITGB3) T779 Uniprot
P08559 (PDHA1) S232 Uniprot
P08559 (PDHA1) S293 Uniprot
P08559 (PDHA1) S300 Uniprot
P23443-1 (RPS6KB1) T390 Uniprot
P29803 (PDHA2) S230 Uniprot
P29803 (PDHA2) S291 Uniprot
P29803 (PDHA2) S298 Uniprot
P31749-1 (AKT1) T308 Uniprot
P31749-1 (AKT1) S473 Uniprot
P31751 (AKT2) T309 Uniprot
Q15118-1 (PDK1) S25 Uniprot
Q15118-1 (PDK1) S393 Uniprot
Q15118-1 (PDK1) S396 Uniprot
Q16513 (PKN2) T816 Uniprot
Q16513-1 (PKN2) T951 Uniprot
Q9Y243-1 (AKT3) S120 Uniprot
Q9Y243 (AKT3) T305 Uniprot

Research Backgrounds

Function:

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

PTMs:

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

Subunit Structure:

Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).

Family&Domains:

Belongs to the PDK/BCKDK protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Pimozide inhibits the growth of breast cancer cells by alleviating the Warburg effect through the P53 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35658233) [IF=7.5]

Application: WB    Species: Human    Sample: MCF-7 cells

Fig. 3. Pimozide inhibits PKM2 protein and mRNA in both MCF-7 and MDA-MB-231 cells in vitro. (A-B) Cells were treated with the indicated concentrations of Pimozide for 24 h, and the protein expression of glycolytic enzymes in MCF-7(A) and MDA-MB-231(B) cells were determined by Western blot analysis (left panel). Densitometry analysis was performed to assess the glycolytic enzymes protein expression (normalized to β-actin expression), PKM2 decreased significantly compared with untreated cells (right panel). (C-D) The mRNA expression of PKM2 in MCF-7(C) and MDA-MB-231(D) cells untreated or treated with Pimozide was determined by qRT-PCR. GAPDH was used as a control. Data represent mean ± SD from three biological replicates (*p < 0.05, **p < 0.01).
2). Lactate accumulation induced by Akt2-PDK1 signaling promotes pulmonary fibrosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024 (PubMed: 38226859) [IF=4.8]
3). Sevoflurane preconditioning attenuates hypoxia/reoxygenation injury of H9c2 cardiomyocytes by activation of the HIF-1/PDK-1 pathway. PeerJ, 2023 (PubMed: 33391885) [IF=2.7]

Application: WB    Species: Rat    Sample: H9c2 cardiomyocytes

Figure 5 SPC upregulate the protein expression of HIF-1a and PDK-1. (A) Western blot image of HIF-1a protein. (B) Western blot image of PDK-1 protein. (C) HIF-1a protein expression. (D) PDK-1 protein expression. Protein content was normalized to GAPDH. Data represent mean ± SD (n = 3/group). *P < 0.05 vs Con, #P < 0.05 vs H/R, &P < 0.05 vs SPC.

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