C9 Antibody - #DF3964

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Product: C9 Antibody
Catalog: DF3964
Description: Rabbit polyclonal antibody to C9
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 70 KD; 63kD(Calculated).
Uniprot: P02748
RRID: AB_2836317

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(80%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
C9 Antibody detects endogenous levels of total C9.
RRID:
AB_2836317
Cite Format: Affinity Biosciences Cat# DF3964, RRID:AB_2836317.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C9; C9 deficiency; C9 deficiency with dermatomyositis; CO9_HUMAN; Complement component 9; Complement component 9 deficiency; Complement component C9; Complement component C9b;

Immunogens

Immunogen:
Uniprot:

P02748(CO9_HUMAN) >>Visit HPA database.

Gene(ID):
C9(735)
Expression:
P02748 CO9_HUMAN:

Plasma (at protein level).

Sequence:
MSACRSFAVAICILEISILTAQYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
82
Dog
82
Horse
80
Bovine
73
Sheep
73
Xenopus
45
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02748 As Substrate

Site PTM Type Enzyme
W48 C-Glycosylation
W51 C-Glycosylation
S66 Phosphorylation
K225 Ubiquitination
T244 Phosphorylation
T248 Phosphorylation
S261 Phosphorylation
N277 N-Glycosylation
T316 Phosphorylation
Y383 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
N415 N-Glycosylation
K435 Ubiquitination

Research Backgrounds

Function:

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

PTMs:

Thrombin cleaves factor C9 to produce C9a and C9b.

Phosphorylation sites are present in the extracellular medium.

Initially, positions and connectivity of disulfide bonds were based on peptide sequencing done for the human protein. The crystal structures for the human and mouse proteins corrected the positions and connectivities of the disulfide bonds. The distance between Cys-57 and Cys-94 in the monomeric mouse protein precludes formation of a disulfide bond, contrary to what is seen in the structure of the human polymeric form of the protein (Probable).

Subcellular Location:

Secreted. Target cell membrane>Multi-pass membrane protein.
Note: Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma (at protein level).

Subunit Structure:

Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. About 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes.

Family&Domains:

Belongs to the complement C6/C7/C8/C9 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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