C6 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A549 cells using C6 antibody.

Product:	C6 Antibody
Catalog:	DF3963
Description:	Rabbit polyclonal antibody to C6
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Horse, Sheep, Dog
Mol.Wt.:	105 KD; 105kD(Calculated).
Uniprot:	P13671
RRID:	AB_2836316

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Horse(82%), Sheep(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

C6 Antibody detects endogenous levels of total C6.

RRID:

AB_2836316
Cite Format: Affinity Biosciences Cat# DF3963, RRID:AB_2836316.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AW111623; C6; CO6_HUMAN; Complement component 6; Complement component C6;

Immunogens

Immunogen:

Uniprot:

P13671(CO6_HUMAN) >>Visit HPA database.

Gene(ID):

C6(729)

Sequence:

P13671 CO6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARRSVLYFILLNALINKGQACFCDHYAWTQWTSCSKTCNSGTQSRHRQIVVDKYYQENFCEQICSKQETRECNWQRCPINCLLGDFGPWSDCDPCIEKQSKVRSVLRPSQFGGQPCTAPLVAFQPCIPSKLCKIEEADCKNKFRCDSGRCIARKLECNGENDCGDNSDERDCGRTKAVCTRKYNPIPSVQLMGNGFHFLAGEPRGEVLDNSFTGGICKTVKSSRTSNPYRVPANLENVGFEVQTAEDDLKTDFYKDLTSLGHNENQQGSFSSQGGSSFSVPIFYSSKRSENINHNSAFKQAIQASHKKDSSFIRIHKVMKVLNFTTKAKDLHLSDVFLKALNHLPLEYNSALYSRIFDDFGTHYFTSGSLGGVYDLLYQFSSEELKNSGLTEEEAKHCVRIETKKRVLFAKKTKVEHRCTTNKLSEKHEGSFIQGAEKSISLIRGGRSEYGAALAWEKGSSGLEEKTFSEWLESVKENPAVIDFELAPIVDLVRNIPCAVTKRNNLRKALQEYAAKFDPCQCAPCPNNGRPTLSGTECLCVCQSGTYGENCEKQSPDYKSNAVDGQWGCWSSWSTCDATYKRSRTRECNNPAPQRGGKRCEGEKRQEEDCTFSIMENNGQPCINDDEEMKEVDLPEIEADSGCPQPVPPENGFIRNEKQLYLVGEDVEISCLTGFETVGYQYFRCLPDGTWRQGDVECQRTECIKPVVQEVLTITPFQRLYRIGESIELTCPKGFVVAGPSRYTCQGNSWTPPISNSLTCEKDTLTKLKGHCQLGQKQSGSECICMSPEEDCSHHSEDLCVFDTDSNDYFTSPACKFLAEKCLNNQQLHFLHIGSCQDGRQLEWGLERTRLSSNSTKKESCGYDTCYDWEKCSASTSKCVCLLPPQCFKGGNQLYCVKMGSSTSEKTLNICEVGTIRCANRKMEILHPGKCLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Sheep

Dog

Pig

Bovine

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13671 As Substrate

P13671

Site	PTM Type	Source
W29	C-Glycosylation	Uniprot
W32	C-Glycosylation	Uniprot
T38	O-Glycosylation	Uniprot
S41	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
W90	C-Glycosylation	Uniprot
S278	Phosphorylation	Uniprot
S311	Phosphorylation	Uniprot
N324	N-Glycosylation	Uniprot
T392	O-Glycosylation	Uniprot
S561	Phosphorylation	Uniprot
W568	C-Glycosylation	Uniprot
W571	C-Glycosylation	Uniprot
W574	C-Glycosylation	Uniprot
S742	Phosphorylation	Uniprot
T767	Phosphorylation	Uniprot
N855	N-Glycosylation	Uniprot

Research Backgrounds

P13671_CO6_HUMAN

Function:

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.

PTMs:

All cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module.

Subcellular Location:

Secreted.

Subunit Structure:

Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and 12-14 copies of the pore-forming subunit C9.

Family&Domains:

Belongs to the complement C6/C7/C8/C9 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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C6 Antibody - #DF3963