C1QB Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells, using C1QB antibody.

Product:	C1QB Antibody
Catalog:	DF3958
Description:	Rabbit polyclonal antibody to C1QB
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	28 KD; 27kD(Calculated).
Uniprot:	P02746
RRID:	AB_2836311

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Dog(86%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

C1QB Antibody detects endogenous levels of total C1QB.

RRID:

AB_2836311
Cite Format: Affinity Biosciences Cat# DF3958, RRID:AB_2836311.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C1qb; C1QB_HUMAN; Complement C1q subcomponent subunit B; Complement component 1 q subcomponent B chain; Complement component 1 q subcomponent beta polypeptide; Complement component C1q B chain; Complement subcomponent C1q chain B;

Immunogens

Immunogen:

Uniprot:

P02746(C1QB_HUMAN) >>Visit HPA database.

Gene(ID):

C1QB(713)

Sequence:

P02746 C1QB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMMKIPWGSIPVLMLLLLLGLIDISQAQLSCTGPPAIPGIPGIPGTPGPDGQPGTPGIKGEKGLPGLAGDHGEFGEKGDPGIPGNPGKVGPKGPMGPKGGPGAPGAPGPKGESGDYKATQKIAFSATRTINVPLRRDQTIRFDHVITNMNNNYEPRSGKFTCKVPGLYYFTYHASSRGNLCVNLMRGRERAQKVVTFCDYAYNTFQVTTGGMVLKLEQGENVFLQATDKNSLLGMEGANSIFSGFLLFPDMEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

Bovine

Sheep

Chicken

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02746 As Substrate

P02746

Site	PTM Type	Source
K77	Methylation	Uniprot
K88	Methylation	Uniprot
K92	Methylation	Uniprot
S125	Phosphorylation	Uniprot
T127	Phosphorylation	Uniprot
T129	Phosphorylation	Uniprot
Y168	Phosphorylation	Uniprot

Research Backgrounds

P02746_C1QB_HUMAN

Function:

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

PTMs:

Hydroxylated on lysine and proline residues. Hydroxylated lysine residues can be glycosylated. Human C1Q contains up to 68.3 hydroxylysine-galactosylglucose residues and up to 2.5 hydroxylysine-galactose per molecule. Total percentage hydroxylysine residues glycosylated is 86.4%.

Subcellular Location:

Secreted.

Subunit Structure:

C1 is a calcium-dependent trimolecular complex of C1q, c1r and C1s in the molar ration of 1:2:2. C1q subcomponent is composed of nine subunits, six of which are disulfide-linked dimers of the A and B chains, and three of which are disulfide-linked dimers of the C chain.

Research Fields

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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For Research Use Only.
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C1QB Antibody - #DF3958