ADH7 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat heart, using ADH7 Antibody.

DF3753 at 1/100 staining human gastric cancer by IHC-P.

Product:	ADH7 Antibody
Catalog:	DF3753
Description:	Rabbit polyclonal antibody to ADH7
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Bovine
Mol.Wt.:	40 KD; 41kD(Calculated).
Uniprot:	P40394
RRID:	AB_2836117

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(92%)

Clonality:

Polyclonal

Specificity:

ADH7 Antibody detects endogenous levels of total ADH7.

RRID:

AB_2836117
Cite Format: Affinity Biosciences Cat# DF3753, RRID:AB_2836117.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADH 4; ADH 7; ADH, class IV; ADH4; Alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide; alcohol dehydrogenase 7; alcohol dehydrogenase class 4 mu/sigma chain; Alcohol dehydrogenase class IV mu/sigma chain; alcohol dehydrogenase VII; class IV sigma 1 alcohol dehydrogenase; class IV sigmasigma alcohol dehydrogenase; Gastric alcohol dehydrogenase; Gastric H(+)/K(+) ATPase subunit alpha; Retinol dehydrogenase;

Immunogens

Immunogen:

Uniprot:

P40394(ADH7_HUMAN) >>Visit HPA database.

Gene(ID):

ADH7(131)

Expression:

P40394 ADH7_HUMAN:

Preferentially expressed in stomach.

Sequence:

P40394 ADH7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40394 As Substrate

P40394

Site	PTM Type	Source
T139	Phosphorylation	Uniprot
T250	Phosphorylation	Uniprot
S254	Phosphorylation	Uniprot

Research Backgrounds

P40394_ADH7_HUMAN

Function:

Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives. Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives. Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal. Catalyzes in the oxidative direction with higher efficiency. Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Preferentially expressed in stomach.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ADH7 Antibody - #DF3753