Histone H2AZ Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Histone H2AZ Antibody.

Product:	Histone H2AZ Antibody
Catalog:	DF3083
Description:	Rabbit polyclonal antibody to Histone H2AZ
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	13 KD; 14kD(Calculated).
Uniprot:	P0C0S5
RRID:	AB_2835466

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Histone H2AZ Antibody detects endogenous levels of total Histone H2AZ.

RRID:

AB_2835466
Cite Format: Affinity Biosciences Cat# DF3083, RRID:AB_2835466.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

H2A histone family member Z; H2A.z; H2A/z; H2afz; H2AZ; H2AZ_HUMAN; Histone H2A.Z; MGC117173;

Immunogens

Immunogen:

Uniprot:

P0C0S5(H2AZ_HUMAN) >>Visit HPA database.

Gene(ID):

H2AFZ(3015)

Sequence:

P0C0S5 H2AZ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Xenopus

Chicken

Pig

Horse

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P0C0S5 As Substrate

P0C0S5

Site	PTM Type	Source
K5	Acetylation	Uniprot
K5	Methylation	Uniprot
K8	Acetylation	Uniprot
K8	Methylation	Uniprot
K12	Acetylation	Uniprot
K14	Acetylation	Uniprot
K121	Ubiquitination	Uniprot
K122	Ubiquitination	Uniprot

Research Backgrounds

P0C0S5_H2AZ_HUMAN

Function:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

PTMs:

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.

Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (By similarity).

Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.

Not phosphorylated.

Subcellular Location:

Nucleus. Chromosome.

Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B. H2AZ1 interacts with INCENP (By similarity). Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AZ1 from the nucleosome. Heterodimer H2BC11 and H2AZ1 interacts with VPS72 (via N-terminal domain). Interacts with PWWP2A. Interacts with FH (when phosphorylated by PRKDC).

Family&Domains:

Belongs to the histone H2A family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

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Histone H2AZ Antibody - #DF3083