Phospho-PGK1 (Thr9) Antibody | Affinity Biosciences

Product:	Phospho-PGK1 (Thr9) Antibody
Catalog:	AF2009
Description:	Rabbit polyclonal antibody to Phospho-PGK1 (Thr9)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	45kDa; 45kD(Calculated).
Uniprot:	P00558

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

Elisa(peptide) 1:20000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-PGK1 (Thr9) Antibody detects endogenous levels of PGK1 only when phosphorylated at T9.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2;

Immunogens

Immunogen:

A synthesized peptide derived from human PGK1 around the phosphorylation site of T9.

Uniprot:

P00558(PGK1_HUMAN) >>Visit HPA database.

Gene(ID):

PGK1(5230)

Expression:

P00558 PGK1_HUMAN:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

Description:

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

Sequence:

P00558 PGK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

PTMs - P00558 As Substrate

P00558

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
S4	Phosphorylation	Uniprot
K6	Acetylation	Uniprot
K6	Ubiquitination	Uniprot
K11	Acetylation	Uniprot
K11	Ubiquitination	Uniprot
K15	Ubiquitination	Uniprot
K17	Ubiquitination	Uniprot
K30	Acetylation	Uniprot
K30	Methylation	Uniprot
K30	Sumoylation	Uniprot
K30	Ubiquitination	Uniprot
T35	Phosphorylation	Uniprot
R39	Methylation	Uniprot
K41	Ubiquitination	Uniprot
S46	Phosphorylation	Uniprot
K48	Acetylation	Uniprot
K48	Ubiquitination	Uniprot
C50	S-Nitrosylation	Uniprot
K56	Acetylation	Uniprot
K56	Ubiquitination	Uniprot
K75	Acetylation	Uniprot
K75	Ubiquitination	Uniprot
Y76	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
K86	Acetylation	Uniprot
K86	Ubiquitination	Uniprot
S87	Phosphorylation	Uniprot
K91	Acetylation	Uniprot
K91	Ubiquitination	Uniprot
K97	Acetylation	Uniprot
K97	Ubiquitination	Uniprot
C99	S-Nitrosylation	Uniprot
K106	Acetylation	Uniprot
K106	Ubiquitination	Uniprot
C108	S-Nitrosylation	Uniprot
S115	Phosphorylation	Uniprot
R123	Methylation	Uniprot
K131	Acetylation	Uniprot
K131	Ubiquitination	Uniprot
K133	Acetylation	Uniprot
S136	Phosphorylation	Uniprot
K139	Ubiquitination	Uniprot
K141	Acetylation	Uniprot
K141	Ubiquitination	Uniprot
K146	Acetylation	Uniprot
K146	Ubiquitination	Uniprot
S153	Phosphorylation	Uniprot
S155	Phosphorylation	Uniprot
K156	Acetylation	Uniprot
K156	Ubiquitination	Uniprot
Y161	Phosphorylation	Uniprot
T168	Phosphorylation	Uniprot
S175	Phosphorylation	Uniprot
K184	Ubiquitination	Uniprot
K191	Acetylation	Uniprot
K191	Methylation	Uniprot
K191	Ubiquitination	Uniprot
K192	Ubiquitination	Uniprot
Y196	Phosphorylation	Uniprot
K199	Acetylation	Uniprot
K199	Ubiquitination	Uniprot
S203	Phosphorylation	Uniprot
K216	Acetylation	Uniprot
K216	Ubiquitination	Uniprot
K220	Acetylation	Uniprot
K220	Ubiquitination	Uniprot
T243	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
K264	Acetylation	Uniprot
K264	Ubiquitination	Uniprot
K267	Acetylation	Uniprot
K267	Ubiquitination	Uniprot
S271	Phosphorylation	Uniprot
K272	Acetylation	Uniprot
K272	Ubiquitination	Uniprot
K275	Acetylation	Uniprot
K279	Ubiquitination	Uniprot
T281	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
K291	Acetylation	Uniprot
K291	Ubiquitination	Uniprot
K297	Ubiquitination	Uniprot
S305	Phosphorylation	Uniprot
K322	Acetylation	Uniprot
K322	Ubiquitination	Uniprot
K323	Acetylation	Uniprot
K323	Ubiquitination	Uniprot
K332	Ubiquitination	Uniprot
K353	Acetylation	Uniprot
K353	Ubiquitination	Uniprot
K361	Acetylation	Uniprot
K361	Ubiquitination	Uniprot
R365	Methylation	Uniprot
C367	S-Nitrosylation	Uniprot
T378	Phosphorylation	Uniprot
C379	S-Nitrosylation	Uniprot
C380	S-Nitrosylation	Uniprot
K382	Acetylation	Uniprot
K382	Ubiquitination	Uniprot
K388	Acetylation	Uniprot
K388	Ubiquitination	Uniprot
S399	Phosphorylation	Uniprot
K406	Acetylation	Uniprot
K406	Ubiquitination	Uniprot
S415	Phosphorylation	Uniprot

PTMs - P00558 As Enzyme

P00558

Substrate	Site	Source
Q15118 (PDK1)	T338	Uniprot

Research Backgrounds

P00558_PGK1_HUMAN

Function:

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the phosphoglycerate kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

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For Research Use Only.
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Phospho-PGK1 (Thr9) Antibody - #AF2009