b-Enolase Antibody - #AF4099

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Product: b-Enolase Antibody
Catalog: AF4099
Description: Rabbit polyclonal antibody to b-Enolase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 45kd; 47kD(Calculated).
Uniprot: P13929
RRID: AB_2835357

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 50ul $250 In stock
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 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
b-Enolase Antibody detects endogenous levels of total b-Enolase.
RRID:
AB_2835357
Cite Format: Affinity Biosciences Cat# AF4099, RRID:AB_2835357.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2 phospho D glycerate hydrol yase; 2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Beta enolase; Beta-enolase; ENO3; ENOB_HUMAN; Enolase 3 (beta muscle); Enolase 3; Enolase3; GSD13; MSE; Muscle specific enolase; Muscle-specific enolase; Skeletal muscle enolase;

Immunogens

Immunogen:
Uniprot:

P13929(ENOB_HUMAN) >>Visit HPA database.

Gene(ID):
ENO3(2027)
Expression:
P13929 ENOB_HUMAN:

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Description:
Appears to have a function in striated muscle development and regeneration.
Sequence:
MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Chicken
92
Rabbit
92
Xenopus
83
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13929 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K28 Ubiquitination
S37 Phosphorylation
S40 Phosphorylation
T41 Phosphorylation
Y44 Phosphorylation
R50 Methylation
K60 Acetylation
K60 Ubiquitination
T72 Phosphorylation
S83 Phosphorylation
S104 Phosphorylation
K105 Ubiquitination
K126 Ubiquitination
Y131 Phosphorylation
S176 Phosphorylation
S177 Phosphorylation
Y189 Phosphorylation
K202 Ubiquitination
T229 Phosphorylation
Y252 Phosphorylation
Y257 Phosphorylation
K262 Acetylation
K262 Ubiquitination
S263 Phosphorylation
K275 Ubiquitination
K281 Ubiquitination
S282 Phosphorylation
Y287 Phosphorylation
K326 Ubiquitination
S349 Phosphorylation
S353 Phosphorylation
C357 S-Nitrosylation
K358 Ubiquitination
S362 Phosphorylation
S373 Phosphorylation
T390 Phosphorylation
K394 Ubiquitination
K406 Ubiquitination
K420 Ubiquitination

Research Backgrounds

Function:

Appears to have a function in striated muscle development and regeneration.

Subcellular Location:

Cytoplasm.
Note: Localized to the Z line. Some colocalization with CKM at M-band (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Subunit Structure:

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Interacts with PNKD.

Family&Domains:

Belongs to the enolase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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