Phospho-RPS6 (Ser235) Antibody-Fluor647 - #AF3354-F647

Product: Phospho-RPS6 (Ser235) Antibody-Fluor647
Catalog: AF3354-F647
Description: Rabbit polyclonal antibody to Phospho-RPS6 (Ser235)-Fluor647
Application: WB IF/ICC FACS
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32kDa; 29kD(Calculated).
Uniprot: P62753

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, FACS 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-RPS6 (Ser235) Antibody detects endogenous levels of RPS6 only when phosphorylated at Serine 235.
Conjugate:
Fluor647. Ex:651nm/Em:667nm
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;

Immunogens

Immunogen:

A synthesized peptide derived from human RPS6 around the phosphorylation site of Ser235.

Uniprot:

P62753(RS6_HUMAN) >>Visit HPA database.

Gene(ID):
RPS6(6194)
Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit.
Sequence:
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

PTMs - P62753 As Substrate

Site PTM Type Enzyme
K2 Methylation
S6 Phosphorylation
C12 S-Nitrosylation
K14 Acetylation
K14 Ubiquitination
R22 Methylation
K23 Ubiquitination
Y28 Phosphorylation
K30 Acetylation
K30 Ubiquitination
K46 Ubiquitination
S53 Phosphorylation
K58 Ubiquitination
K64 Ubiquitination
T69 Phosphorylation
S78 Phosphorylation
K79 Acetylation
K79 Ubiquitination
S82 Phosphorylation
K116 Ubiquitination
K119 Ubiquitination
T128 Phosphorylation
K143 Ubiquitination
S148 Phosphorylation
K149 Ubiquitination
R154 Methylation
K160 Ubiquitination
T181 Phosphorylation
K203 Ubiquitination
Y209 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K218 Acetylation
S235 Phosphorylation Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1)
S236 Phosphorylation Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK)
S240 Phosphorylation Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
T241 Phosphorylation
S242 Phosphorylation Q13177 (PAK2)
S244 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S246 Phosphorylation
S247 Phosphorylation P23443 (RPS6KB1)

Research Backgrounds

Function:

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

PTMs:

Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS6 family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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