DTX1 Antibody | Affinity Biosciences

Product:	DTX1 Antibody
Catalog:	DF15262
Description:	Rabbit polyclonal antibody to DTX1
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	67kD(Calculated).
Uniprot:	Q86Y01

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

DTX1 Antibody detects endogenous levels of DTX1.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deltex 1, E3 ubiquitin ligase; Deltex; Deltex homolog 1 (Drosophila); Deltex homolog 1; Deltex protein 1; Deltex-1; Deltex1; dtx1; DTX1_HUMAN; E3 ubiquitin protein ligase DTX1; E3 ubiquitin-protein ligase DTX1; FXI-T1; Fxit 1; Fxit1; hDTX1; hDx 1; mDTX1; Protein deltex 1; Protein deltex-1;

Immunogens

Immunogen:

A synthesized peptide derived from human DTX1.

Uniprot:

Q86Y01(DTX1_HUMAN) >>Visit HPA database.

Gene(ID):

DTX1(1840)

Expression:

Q86Y01 DTX1_HUMAN:

Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.

Sequence:

Q86Y01 DTX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGGPPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQRTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRHKGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFPDTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAAAKA

Research Backgrounds

Q86Y01_DTX1_HUMAN

Function:

Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.

PTMs:

Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.

Tissue Specificity:

Subunit Structure:

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminal region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH.

Family&Domains:

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.

Belongs to the Deltex family.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)

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DTX1 Antibody - #DF15262