STAT2 Antibody - #AF6342

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Product: STAT2 Antibody
Catalog: AF6342
Description: Rabbit polyclonal antibody to STAT2
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 113kDa; 98kD(Calculated).
Uniprot: P52630
RRID: AB_2835198

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(82%), Horse(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
STAT2 Antibody detects endogenous levels of total STAT2.
RRID:
AB_2835198
Cite Format: Affinity Biosciences Cat# AF6342, RRID:AB_2835198.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF 3; ISGF3; MGC59816; P113; signal transducer and activator of transcription 2 113kD; Signal transducer and activator of transcription 2; STAT113; Stat2; STAT2_HUMAN;

Immunogens

Immunogen:
Uniprot:

P52630(STAT2_HUMAN) >>Visit HPA database.

Gene(ID):
STAT2(6773)
Description:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
Sequence:
MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Pig
82
Horse
82
Bovine
70
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52630 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
T136 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K161 Ubiquitination
K167 Ubiquitination
K184 Ubiquitination
S187 Phosphorylation
K194 Ubiquitination
K197 Ubiquitination
K208 Ubiquitination
K218 Ubiquitination
K239 Ubiquitination
K244 Ubiquitination
K280 Ubiquitination
S283 Phosphorylation
S287 Phosphorylation
T294 Phosphorylation
K295 Ubiquitination
K375 Acetylation
K375 Ubiquitination
K384 Acetylation
K384 Ubiquitination
T387 Phosphorylation
K443 Ubiquitination
K523 Ubiquitination
K543 Ubiquitination
K550 Ubiquitination
S595 Phosphorylation
T597 Phosphorylation
Y631 Phosphorylation
K681 Ubiquitination
Y690 Phosphorylation P06239 (LCK)
S699 Phosphorylation
S734 Phosphorylation
S753 Phosphorylation
T800 Phosphorylation
Y833 Phosphorylation
S849 Phosphorylation

Research Backgrounds

Function:

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.

PTMs:

Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response.

'Lys-48'-linked ubiquitination by DCST1 leads to STAT2 proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus upon activation by IFN-alpha/beta.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation (By similarity). The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9; interacts with IRF9 in the cytoplasm (By similarity). Interacts with CRSP2 and CRSP6. Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with ARL2BP (By similarity). Interacts with E3 ubiquitin ligase DCST1; the interaction results in STAT2 ubiquitin-mediated proteasomal degradation.

(Microbial infection) Interacts with vaccinia virus protein C6.

(Microbial infection) Interacts with Simian virus 5 protein V.

(Microbial infection) Interacts with Rabies virus phosphoprotein.

(Microbial infection) Interacts with Human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth.

(Microbial infection) Interacts with Dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). A Novel Strategy for Regulating mRNA’s Degradation via Interfering the AUF1’s Binding to mRNA. Molecules, 2022 (PubMed: 35630659) [IF=4.6]

Application: WB    Species: Human    Sample: SK-MEL-2 cells

Figure 5 JNJ-7706621 regulated IL-8 gene’s translation via interacting with AUF1 and inhibited tumor cells’ proliferation. (a) The remaining amount of IL8 mRNA after treated with 1.75 μM JNJ-7706621 for 24 h in SK-MEL-2 cells. Actinomycin D (5 μg/mL) was added to stop the transcription to detect remaining IL8 mRNA with qRT-PCR. The fitting curve was performed using GraphPad Prism. (b) The REMSA result of AUF1 (750 nM) incubated with the FAM-labelled RNA at 250 nM in the presence of JNJ-7706621. The sample was incubated in an incubation buffer (10 mM Tris-HCl, pH 8.0, containing 50 mM KCl, 2 mM DTT, 0.5 mM EDTA, and 0.1 µg/µL BSA) and loaded on a 6% native polyacrylamide gel. (c) The wild-type plasmid (3′WT) and mutant plasmid (3′del) were transfected in SK-MEL-2 cells. After incubation, the relative luciferase activity (RL/FL) was detected and normalized with the data of 3′del. (d) The relative luciferase activity of 3′WT and 3′WT dual-luciferase reporters after treated with JNJ-7706621 (1.75 μM), normalizing with the data of 3′WT. (e) The expression of IL8 in SK-MEL-2 cells treated with 1.75 and 3 μM JNJ-7706621. (f) The expression of IL8 in SK-MEL-2 cells treated with 80 nM siRNA of AUF1. JNJ-7706621 was added after 10-h pre-incubation with the siRNA. (g) Inhibitory effect of JNJ-7706621 in different cancer cells for 48 h in the MTT assay. All experiments were parallelly repeated in triplicate, and data are shown as means ± SEM of n = 3.

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