Product: NMDAR2B Antibody
Catalog: AF6426
Description: Rabbit polyclonal antibody to NMDAR2B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 180kDa; 166kD(Calculated).
Uniprot: Q13224
RRID: AB_2835159

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(91%), Bovine(91%), Horse(91%), Rabbit(91%), Dog(91%), Xenopus(91%)
NMDAR2B Antibody detects endogenous levels of total NMDAR2B.
Cite Format: Affinity Biosciences Cat# AF6426, RRID:AB_2835159.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


AW490526; EIEE27; Glutamate [NMDA] receptor subunit epsilon 2; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B; Glutamate receptor ionotropic NMDA2B; Glutamate receptor subunit epsilon 2; Glutamate receptor, ionotropic, NMDA2B (epsilon 2); GRIN 2B; GRIN2B; hNR 3; hNR3; MGC142178; MGC142180; MRD6; N methyl D asparate receptor channel subunit epsilon 2; N methyl D aspartate receptor subtype 2B; N methyl D aspartate receptor subunit 2B; N methyl D aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDA NR2B; NMDA R2B; NMDAR2B; NMDE2; NMDE2_HUMAN; NME2; NR2B; NR3;



Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13224 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
S34 Phosphorylation
Y239 Phosphorylation
S383 Phosphorylation Q13554 (CAMK2B) , Q9UQM7 (CAMK2A)
T475 Phosphorylation
T493 Phosphorylation
T514 Phosphorylation
S720 Phosphorylation
S759 Phosphorylation
T760 Phosphorylation
Y762 Phosphorylation
S770 Phosphorylation
S865 Phosphorylation
Y932 Phosphorylation P06241 (FYN)
Y1039 Phosphorylation P06241 (FYN)
S1043 Phosphorylation
Y1070 Phosphorylation P06241 (FYN)
Y1109 Phosphorylation P06241 (FYN)
S1116 Phosphorylation
S1166 Phosphorylation
S1168 Phosphorylation
T1183 Phosphorylation
S1191 Phosphorylation
Y1252 Phosphorylation P06241 (FYN)
K1280 Acetylation
S1284 Phosphorylation
K1289 Acetylation
S1303 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB) , Q13554 (CAMK2B) , P53355 (DAPK1) , P05129 (PRKCG) , Q9UQM7 (CAMK2A)
Y1304 Phosphorylation
T1306 Phosphorylation
S1323 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
Y1336 Phosphorylation P06241 (FYN)
T1410 Phosphorylation
S1432 Phosphorylation
S1469 Phosphorylation
Y1474 Phosphorylation P29323 (EPHB2) , P12931 (SRC) , P06241 (FYN)
S1478 Phosphorylation
S1479 Phosphorylation P68400 (CSNK2A1)
S1482 Phosphorylation

Research Backgrounds


Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).


Phosphorylated on tyrosine residues (By similarity). Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Late endosome. Lysosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.

Subunit Structure:

Heterotetramer. Forms heterotetrameric channels composed of two zeta subunits (GRIN1), and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro). Can also form heterotetrameric channels that contain at least one zeta subunit (GRIN1), at least one epsilon subunit, plus GRIN3A or GRIN3B (By similarity). In vivo, the subunit composition may depend on the expression levels of the different subunits (Probable). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of PATJ, DLG3 and DLG4. Interacts with HIP1 and NETO1 (By similarity). Interacts with MAGI3. Interacts with DAPK1 (By similarity). Found in a complex with GRIN1 and PRR7. Interacts with PRR7. Interacts with CAMK2A. Interacts with ARC; preventing ARC oligomerization (By similarity). Interacts with TMEM25 (By similarity).


A hydrophobic region that gives rise to the prediction of a transmembrane span does not cross the membrane, but is part of a discontinuously helical region that dips into the membrane and is probably part of the pore and of the selectivity filter.

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.


1). Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function, 2022 (PubMed: 35899807) [IF=6.1]

2). A decrease in NR2B expression mediated by DNA hypermethylation induces perioperative neurocognitive disorder in aged mice. CNS Neuroscience & Therapeutics, 2023 (PubMed: 36694341) [IF=5.5]

3). Activation of the PACAP/PAC1 Signaling Pathway Accelerates the Repair of Impaired Spatial Memory Caused by an Ultradian Light Cycle. ASN Neuro, 2023 (PubMed: 37071544) [IF=4.7]

Application: WB    Species: Mouse    Sample:

Figure 3. The ultradian light cycle disrupts the rhythmic expression of AMPA receptors and NMDA receptors in the CA1 region. A, C, E, G, Representative immunoblots showing expression of GluR1, GluR2, NR2A, and NR2B in CA1 from T24-housed (black, N = 4) and T7-housed (gray, N = 4) mice (GluR1: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit 1; GluR2: AMPA receptor subunit 2; NR2A: N-methyl-D-aspartate (NMDA) receptor subunits NR2A; NR2B: NMDB receptor subunits NR2B). B,T24 vs T7 has a different expression of GluR1(The data were analyzed using two-way ANOVA (F(1,36) = 13.15, *** P  0.05). F, No differences in the rhythmicity of NR2A levels were observed between groups two-way ANOVA: P > 0.05). H, No differences in the rhythmicity of NR2B levels were observed between groups two-way ANOVA: P > 0.05).

4). Impairment of learning and memory induced by perinatal exposure to BPA is associated with ERα-mediated alterations of synaptic plasticity and PKC/ERK/CREB signaling pathway in offspring rats. BRAIN RESEARCH BULLETIN, 2020 (PubMed: 32380187) [IF=3.8]

Application: IHC    Species: rat    Sample: hippocampus

Fig. 7. |Effects of perinatal exposure to BPA on the levels of protein involved in synaptic plasticity in hippocampus. (A) Immunohistochemistry and the comparison of H-SCORE show the expressions of NR2B, GluA1, PSD-95 and synapsin I. The results are expressed as mean ± SD, n = 3.

5). Sub-chronic exposure to morphine alters general anesthetic potency by differentially regulating the expression of neurotransmitter receptor subunits in mice. Brain Research Bulletin, 2021 (PubMed: 33484757) [IF=3.8]

6). Role of 5-HT1A receptor in insular cortex mediating stress – induced visceral sensory dysfunction. Neurogastroenterology & Motility, 2016 (PubMed: 26969829) [IF=3.5]

Application: WB    Species:    Sample:

Figure 3 Comparison of the expression levels of HTR1As and NR2B in bilateral insular cortexes among three groups. The mRNA and protein expression levels of HTR1As (A and C) and NR2B (B and D) in bilateral insular cortexes were compared among WAS, sham WAS and normal control groups. (E) and (F): Representative immunoblots from the bilateral insular cortexes show the expression of NR2B, 5HTR1A and GAPDH, with an indication of size (kilodaltons, kDa). *represented that the difference was significant, p < 0.05. WAS, water avoidance stress.

7). Enriched environment rescues neonatal pain induced cognitive deficits and the impaired hippocampal synaptic plasticity later in life. Developmental Neurobiology, 2022 (PubMed: 35945168) [IF=3.0]

8). The Effect of Melatonin on Radicular Pain in a Rat Model of Lumbar Disc Herniation. Spine, 2023 (PubMed: 35102121) [IF=3.0]

9). Oral application of magnesium-L-threonate alleviates radicular pain by inhibiting neuro-inflammation dependent central sensitization of rats. Brain research, 2024 (PubMed: 38604557) [IF=2.9]

10). Emotional and Cognitive Impairment Induced by Sleep Deprivation Due to Altered Synaptic Plasticity in the Rat Insula. , 2022

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