ELK1 Antibody | Affinity Biosciences

Cites

1/1

Figure 4The expression analysis of ACAT2 and its downstream factors in dairy cows.

Product:	ELK1 Antibody
Catalog:	AF6213
Description:	Rabbit polyclonal antibody to ELK1
Application:	WB IHC IF/ICC IP
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	62kDa; 45kD(Calculated).
Uniprot:	P19419
RRID:	AB_2835094

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ELK1 Antibody detects endogenous levels of total ELK1.

RRID:

AB_2835094
Cite Format: Affinity Biosciences Cat# AF6213, RRID:AB_2835094.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ELK 1; Elk1; ELK1 member of ETS oncogene family; ELK1 protein; ELK1, ETS transcription factor; ELK1_HUMAN; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene;

Immunogens

Immunogen:

Uniprot:

P19419(ELK1_HUMAN) >>Visit HPA database.

Gene(ID):

ELK1(2002)

Expression:

P19419 ELK1_HUMAN:

Lung and testis.

Description:

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene.

Sequence:

P19419 ELK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPSVTLWQFLLQLLREQGNGHIISWTSRDGGEFKLVDAEEVARLWGLRKNKTNMNYDKLSRALRYYYDKNIIRKVSGQKFVYKFVSYPEVAGCSTEDCPPQPEVSVTSTMPNVAPAAIHAAPGDTVSGKPGTPKGAGMAGPGGLARSSRNEYMRSGLYSTFTIQSLQPQPPPHPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEELEVAGERGFVPETTKAEPEVPPQEGVPARLPAVVMDTAGQAGGHAASSPEISQPQKGRKPRDLELPLSPSLLGGPGPERTPGSGSGSGLQAPGPALTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGSAQVHIPSISVDGLSTPVVLSPGPQKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19419 As Substrate

P19419

Site	PTM Type	Enzyme	Source
Y57	Phosphorylation		Uniprot
K59	Ubiquitination		Uniprot
S160	Phosphorylation		Uniprot
S166	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot
K230	Sumoylation		Uniprot
K249	Sumoylation		Uniprot
K254	Sumoylation		Uniprot
S303	Phosphorylation		Uniprot
S304	Phosphorylation		Uniprot
S324	Phosphorylation	P27361 (MAPK3)	Uniprot
S326	Phosphorylation		Uniprot
T336	Phosphorylation	P27361 (MAPK3)	Uniprot
T353	Phosphorylation		Uniprot
T363	Phosphorylation		Uniprot
T368	Phosphorylation		Uniprot
S383	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14) , P45983 (MAPK8) , Q9UL54 (TAOK2) , O75582 (RPS6KA5)	Uniprot
S389	Phosphorylation	Q16539 (MAPK14) , P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , P45984 (MAPK9)	Uniprot
S416	Phosphorylation		Uniprot
T417	Phosphorylation	Q13523 (PRPF4B)	Uniprot
S422	Phosphorylation	P27361 (MAPK3)	Uniprot

Research Backgrounds

P19419_ELK1_HUMAN

Function:

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).

PTMs:

Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.

On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Lung and testis.

Subunit Structure:

Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9. Interacts with POU1F1.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Subclinical ketosis leads to lipid metabolism disorder by down-regulating the expression of Acetyl-CoA acetyltransferase 2 (ACAT2) in dairy cows. Journal of Dairy Science, 2023 (PubMed: 37690731) [IF=3.5]

Application: WB Species: cows Sample:

Figure 4The expression analysis of ACAT2 and its downstream factors in dairy cows. (A) The relative mRNA expression abundance of ACAT2, PLIN2, and APOB. (B, C) The relative protein expression abundance of ACAT2, PLIN2, and APOB. (D) The concentration of low-density lipoprotein cholesterol (LDL-C) and very low–density lipoprotein (VLDL) in the plasma of dairy cows. Comparisons between 2 groups were calculated using the independent samples t-test. For all bar plots shown, blue represents the control (CON) group and red represents the subclinical ketosis (SCK) group, and the data are expressed as mean ± SEM. * represents a significant difference between the 2 groups (P < 0.05), ** represents an extremely significant difference between the 2 groups (P < 0.01).

2). Subclinical ketosis leads to lipid metabolism disorder by downregulating the expression of acetyl-coenzyme A acetyltransferase 2 in dairy cows. Journal of dairy science, 2023 (PubMed: 37690731) [IF=3.5]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ELK1 Antibody - #AF6213