GOT2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from various samples, using GOT2 Antibody.

DF13922 at 1/100 staining human lung cancer and para-carcinoma tissue by IHC-P.

Product:	GOT2 Antibody
Catalog:	DF13922
Description:	Rabbit polyclonal antibody to GOT2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	48kD(Calculated).
Uniprot:	P00505

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

GOT2 Antibody detects endogenous levels of total GOT2.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AATM_HUMAN; AL022787; Aspartate aminotransferase 2; Aspartate aminotransferase; Aspartate aminotransferase, mitochondrial; Aspartate transaminase 2; ASPATA; EC 2.6.1.1; FABP 1; FABP pm; FABP-1; FABPpm; Fatty acid binding protein; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; Glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase, mitochondrial; Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); Got 2; GOT2; KAT4; KATIV; kynurenine aminotransferase 4; Kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mAspAT; MGC102129; MGC115763; mitAAT; mitochondrial; Mitochondrial aspartate aminotransferase; OTTMUSP00000017748; Plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A;

Immunogens

Immunogen:

A synthesized peptide derived from Human GOT2.

Uniprot:

P00505(AATM_HUMAN) >>Visit HPA database.

Gene(ID):

GOT2(2806)

Sequence:

P00505 AATM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALLHSGRVLPGIAAAFHPGLAAAASARASSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

Research Backgrounds

P00505_AATM_HUMAN

Function:

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.

Subcellular Location:

Mitochondrion matrix. Cell membrane.
Note: Exposure to alcohol promotes translocation to the cell membrane.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine, tyrosine and tryptophan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Digestive system > Fat digestion and absorption.

Restrictive clause

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For Research Use Only.
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GOT2 Antibody - #DF13922