Product: CREB Antibody
Catalog: AF6188
Source: Rabbit
Application: WB, IHC, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43kDa; 37kD(Calculated).
Uniprot: P16220
RRID: AB_2835071

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
CREB Antibody detects endogenous levels of total CREB.
Cite Format: Affinity Biosciences Cat# AF6188, RRID:AB_2835071.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Active transcription factor CREB; cAMP response element binding protein 1; cAMP response element binding protein; cAMP responsive element binding protein 1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; CREB1_HUMAN; Cyclic AMP-responsive element-binding protein 1; MGC9284; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; Transactivator protein;


This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16220 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
S80 Phosphorylation
S98 Phosphorylation Q15139 (PRKD1)
T100 Phosphorylation Q13315 (ATM)
S108 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
S111 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1) , Q13315 (ATM)
S114 Phosphorylation P48729 (CSNK1A1)
S117 Phosphorylation
T119 Phosphorylation
S121 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S129 Phosphorylation P49841 (GSK3B)
S133 Phosphorylation Q16539 (MAPK14) , P31751 (AKT2) , Q02156 (PRKCE) , O43781 (DYRK3) , Q96NX5 (CAMK1G) , Q16566 (CAMK4) , P17612 (PRKACA) , Q6SA08 (TSSK4) , Q99986 (VRK1) , Q13557 (CAMK2D) , Q15418 (RPS6KA1) , O75676 (RPS6KA4) , P28482 (MAPK1) , Q14012 (CAMK1) , Q06187 (BTK) , P51812 (RPS6KA3) , Q13627 (DYRK1A) , O75582 (RPS6KA5) , P41279 (MAP3K8) , O00141 (SGK1) , P27361 (MAPK3) , P31749 (AKT1) , Q13315 (ATM) , Q15349 (RPS6KA2) , Q9Y243 (AKT3) , P49137 (MAPKAPK2) , Q15139 (PRKD1)
Y134 Phosphorylation
K136 Acetylation
K136 Ubiquitination
S142 Phosphorylation Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)
S143 Phosphorylation
S156 Phosphorylation P48729 (CSNK1A1)
T256 Phosphorylation
S270 Phosphorylation P06493 (CDK1)
S271 Phosphorylation Q9H2X6 (HIPK2) , P06493 (CDK1)
K285 Sumoylation
K304 Sumoylation
K309 Ubiquitination
R314 Methylation
K323 Ubiquitination
K330 Ubiquitination
K333 Ubiquitination
K339 Ubiquitination
S340 Phosphorylation

Research Backgrounds


Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.


Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-133.

Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-133. Forms a complex with KMT2A and CREBBP.

(Microbial infection) Interacts with hepatitis B virus/HBV protein X.

(Microbial infection) Interacts with HTLV-1 protein Tax.


Belongs to the bZIP family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.


1). Peng Z et al. MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum. Ecotoxicol Environ Saf 2021 Aug 20;224:112658. (PubMed: 34425535) [IF=7.129]

2). Huang Y et al. LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway. J Exp Clin Cancer Res 2017 Dec 28;36(1):194 (PubMed: 29282102) [IF=7.068]

Application: WB    Species: human    Sample: Gastric cancer cells

Fig. 8 | LncRNA AK023391 was involved in the regulation of the PI3K/Akt signaling pathway.e-f Western blotting validation of the effects of AK023391 knockdown on the expression of PI3K/Akt, NF-κB, p53, and FOXO3a pathways, and their downstream transcription factors c-myb, cyclinB1/G2, and BCL-6 in HGC 27, AGS, and SGC-7901 cells

3). Li S et al. Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY) 2020 Jun 9;12. (PubMed: 32518214) [IF=5.955]

Application: WB    Species: rat    Sample:

Figure 6. |Sal upregulates the FGF2-mediated cAMP/PKA/CREB signaling pathway following MCAO/R. (A–G) Representative western blot bands of cAMP, PKA, CREB, p-CREB, FGF2 and FGFR1 in each group.

4). Chen S et al. The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Front Physiol 2021 Sep 13;12:709135. (PubMed: 34588991) [IF=4.566]

Application: WB    Species: Mice    Sample: gastrocnemius

Figure 3 The time-dependent effects of acute lactate and forskolin injection on the cAMP-PKA pathway. (A) Western blot analysis of cAMP-PKA pathway proteins after acute forskolin injection. (B) Western blot analysis of cAMP-PKA pathway proteins after acute lactate injection. (C,D) The ratios of P-PKA/PKA and P-CREB/CREB after acute forskolin injection. (E,F) The ratios of P-PKA/PKA and P-CREB/CREB after acute lactate injection. AL-15, AL-30, AL-60, and AL-120 represent 15, 30, 60, and 120min after acute lactate injections. AF-15, AF-30, AF-60, and AF-120 represent 15, 30, 60, and 120min after acute forskolin injections. AP and AD mean sacrificed after acute PBS and DMSO injection, respectively. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences between the two groups were analyzed with the independent-samples t-test. * p<0.05 vs. AD and AP.

5). Wu J et al. Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection. Int J Mol Sci 2019 Jan 8;20(1) (PubMed: 30625977) [IF=4.556]

Application: WB    Species: rat    Sample: hippocampus

Figure 6.| ZL activated cAMP-CREB-BDNF signaling. (A–C) Protein expressions of BDNF, p-CREB and CREB in the hippocampus at the end of experiment determined by western blotting.Quantified protein level was normalized to that of GAPDH using densitometry (n = 3)

6). Chen J et al. CREB1 regulates glucose transport of glioma cell line U87 by targeting GLUT1. Mol Cell Biochem 2017 Dec;436(1-2):79-86 (PubMed: 28646353) [IF=3.842]

Application: WB    Species: human    Sample:

Application: IHC    Species: human    Sample:

Fig. 1 The expression of the CREB1, pCREB1, and GLUT1 in normal brain tissue and glioma.

7). Yang F et al. RAGE Signaling pathway in hippocampus dentate gyrus involved in GLT-1 decrease induced by chronic unpredictable stress in rats. Brain Res Bull 2020 Oct;163:49-56. (PubMed: 32621862) [IF=3.715]

Application: WB    Species: rat    Sample: hippocampus

Fig. 4| Both CUS and RAGE knockdown down-regulated the protein and mRNA levels of GLT-1 and phosphorylation of CREB and p65 in hippocampus DG (n = 4 per group). (A)and (B) statistical schematics represented the reduction of protein expression induced by CUS and RAGE knockdown. (C) Representative Western blots.

8). Yang C et al. Intrathecal administration of SRT1720 relieves bone cancer pain by inhibiting the CREB/CRTC1 signalling pathway. Neurosci Lett 2019 Nov 10:134623 (PubMed: 31722235) [IF=3.197]

Application: WB    Species: mouse    Sample: spinal cord

Fig. 3. |Time course of the expression of p-CREB, t-CREB and CRTC1 in the spinal cord of BCP mice. (A) Western blotting of p-CREB, t-CREB and CRTC1.

9). Chen M et al. Acute and chronic effects of high-intensity interval training (HIIT) on postexercise intramuscular lipid metabolism in rats. Physiol Res 2021 Sep 10. (PubMed: 34505529) [IF=2.139]

Application: WB    Species: Rat    Sample:

Fig. 2 (A) Western blot analysis and (B) relative fold protein expression of p-CREB/CREB, p-AMPK/AMPK, and CPT-1b. Relative expression levels were normalized to GAPDH. The acute group’s protein level is normalized to GAPDH at first and then relative to the group of CE, which did not perform the acute training. Three bands are used for statistics. The data were presented as the mean ± standard deviation. Each postexercise timepoint data are compared with CE and significant differences were analyzed with one-way ANOVA. * P<0.05.

10). Activation of the α2A adrenoceptor in microglia promotes LPS-induced TNF-α production and cognitive impairment in mice.

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