CaMKII alpha Antibody | Affinity Biosciences

Product:	CaMKII alpha Antibody
Catalog:	AF6793
Description:	Rabbit polyclonal antibody to CaMKII alpha
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	50~60kDa; 54kD(Calculated).
Uniprot:	Q9UQM7
RRID:	AB_2847516

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CaMK2 alpha Antibody detects endogenous levels of total CaMK2 alpha.

RRID:

AB_2847516
Cite Format: Affinity Biosciences Cat# AF6793, RRID:AB_2847516.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha CaMKII; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha B subunit; Calcium/calmodulin dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMK II alpha subunit; CaMK-II subunit alpha; Camk2a; CAMKA; CaMKII; CaMKIINalpha; EC 2.7.11.17; KCC2A_HUMAN; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320;

Immunogens

Immunogen:

A synthesized peptide derived from human CaMK2 alpha.

Uniprot:

Q9UQM7(KCC2A_HUMAN) >>Visit HPA database.

Gene(ID):

CAMK2A(815)

Sequence:

Q9UQM7 KCC2A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH

PTMs - Q9UQM7 As Substrate

Q9UQM7

Site	PTM Type	Enzyme	Source
C6	S-Nitrosylation		Uniprot
Y13	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
K68	Ubiquitination		Uniprot
S78	Phosphorylation		Uniprot
K137	Ubiquitination		Uniprot
S145	Phosphorylation		Uniprot
K226	Acetylation		Uniprot
K226	Ubiquitination		Uniprot
Y230	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
K245	Ubiquitination		Uniprot
T253	Phosphorylation		Uniprot
K258	Methylation		Uniprot
C280	S-Nitrosylation		Uniprot
T286	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
K298	Methylation		Uniprot
K300	Methylation		Uniprot
T305	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
T306	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
T310	Phosphorylation		Uniprot
S314	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S318	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
T334	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot

PTMs - Q9UQM7 As Enzyme

Q9UQM7

Substrate	Site	Source
A8K161 (CAMK2A)	T286	Uniprot
A8K161 (CAMK2A)	T305	Uniprot
O00631 (SLN)	T5	Uniprot
O14490-2 (DLGAP1)	S44	Uniprot
O14713-2 (ITGB1BP1)	T38	Uniprot
O15273 (TCAP)	S157	Uniprot
O15273 (TCAP)	S161	Uniprot
O95999 (BCL10)	S138	Uniprot
P00533 (EGFR)	S768	Uniprot
P00533-1 (EGFR)	S1064	Uniprot
P00533 (EGFR)	S1070	Uniprot
P00533 (EGFR)	S1071	Uniprot
P00533-1 (EGFR)	S1081	Uniprot
P00533-1 (EGFR)	S1120	Uniprot
P00533 (EGFR)	S1166	Uniprot
P04626 (ERBB2)	T1172	Uniprot
P05556 (ITGB1)	T788	Uniprot
P05556 (ITGB1)	T789	Uniprot
P05783 (KRT18)	S53	Uniprot
P07101 (TH)	S19	Uniprot
P07101-4 (TH)	S35	Uniprot
P07101-3 (TH)	S40	Uniprot
P07101-4 (TH)	S44	Uniprot
P07101 (TH)	S71	Uniprot
P08670 (VIM)	S83	Uniprot
P09917 (ALOX5)	S272	Uniprot
P10636-6 (MAPT)	S204	Uniprot
P10636-8 (MAPT)	S262	Uniprot
P10636-2 (MAPT)	S267	Uniprot
P10636-6 (MAPT)	S298	Uniprot
P10636-8 (MAPT)	S356	Uniprot
P10636-8 (MAPT)	S416	Uniprot
P10636 (MAPT)	T548	Uniprot
P10636 (MAPT)	S552	Uniprot
P10636 (MAPT)	S579	Uniprot
P11831 (SRF)	S103	Uniprot
P11831 (SRF)	T160	Uniprot
P14136-1 (GFAP)	S13	Uniprot
P14136-1 (GFAP)	S17	Uniprot
P14136-1 (GFAP)	S38	Uniprot
P14136-1 (GFAP)	S393	Uniprot
P14921 (ETS1)	S251	Uniprot
P14921 (ETS1)	S257	Uniprot
P14921-1 (ETS1)	S282	Uniprot
P14921 (ETS1)	S285	Uniprot
P15036 (ETS2)	S246	Uniprot
P15036 (ETS2)	S310	Uniprot
P15036 (ETS2)	S313	Uniprot
P16070 (CD44)	S706	Uniprot
P16220 (CREB1)	S142	Uniprot
P16615-2 (ATP2A2)	S38	Uniprot
P16949 (STMN1)	S16	Uniprot
P17676-1 (CEBPB)	S325	Uniprot
P18433 (PTPRA)	S204	Uniprot
P20941-2 (PDC)	S2	Uniprot
P20941-2 (PDC)	S21	Uniprot
P20941-1 (PDC)	S54	Uniprot
P20941-1 (PDC)	S73	Uniprot
P21333 (FLNA)	S2523	Uniprot
P23677 (ITPKA)	T311	Uniprot
P26678 (PLN)	T17	Uniprot
P28329-3 (CHAT)	T456	Uniprot
P28329 (CHAT)	T574	Uniprot
P29475 (NOS1)	S852	Uniprot
P31645 (SLC6A4)	S13	Uniprot
P35222 (CTNNB1)	T332	Uniprot
P35222 (CTNNB1)	T472	Uniprot
P35222 (CTNNB1)	S552	Uniprot
P35270 (SPR)	S213	Uniprot
P35367 (HRH1)	T140	Uniprot
P35367 (HRH1)	T142	Uniprot
P35367 (HRH1)	S396	Uniprot
P35367 (HRH1)	S398	Uniprot
P35372 (OPRM1)	S268	Uniprot
P35372 (OPRM1)	S270	Uniprot
P35372 (OPRM1)	T372	Uniprot
P35462 (DRD3)	S229	Uniprot
P37840 (SNCA)	S129	Uniprot
P42261 (GRIA1)	S567	Uniprot
P42261 (GRIA1)	S832	Uniprot
P46527 (CDKN1B)	S10	Uniprot
P48058 (GRIA4)	S862	Uniprot
P51790 (CLCN3)	S109	Uniprot
P55042 (RRAD)	S273	Uniprot
P55055 (NR1H2)	S426	Uniprot
Q00613-1 (HSF1)	S230	Uniprot
Q00975 (CACNA1B)	S2120	Uniprot
Q01970 (PLCB3)	S537	Uniprot
Q04760 (GLO1)	T107	Uniprot
Q05397 (PTK2)	S843	Uniprot
Q05469 (LIPE)	S855	Uniprot
Q05682 (CALD1)	S643	Uniprot
Q05682 (CALD1)	S656	Uniprot
Q06210 (GFPT1)	S261	Uniprot
Q12879-1 (GRIN2A)	S1291	Uniprot
Q12959 (DLG1)	S232	Uniprot
Q13224 (GRIN2B)	S383	Uniprot
Q13224 (GRIN2B)	S1303	Uniprot
Q14653 (IRF3)	S386	Uniprot
Q14654 (KCNJ11)	T180	Uniprot
Q14654 (KCNJ11)	T224	Uniprot
Q14674 (ESPL1)	S1501	Uniprot
Q15121 (PEA15)	S116	Uniprot
Q15796 (SMAD2)	S110	Uniprot
Q15796-1 (SMAD2)	S240	Uniprot
Q15796-1 (SMAD2)	S260	Uniprot
Q4G163 (FBXO43)	T234	Uniprot
Q86UR5 (RIMS1)	S242	Uniprot
Q86UR5 (RIMS1)	S288	Uniprot
Q8IZP0 (ABI1)	S88	Uniprot
Q92736 (RYR2)	S2808	Uniprot
Q92736 (RYR2)	S2814	Uniprot
Q96A00 (PPP1R14A)	S130	Uniprot
Q96NW7 (LRRC7)	S1439	Uniprot
Q96PH1-4 (NOX5)	S475	Uniprot
Q96PM5 (RCHY1)	T154	Uniprot
Q96PM5 (RCHY1)	S155	Uniprot
Q96PV0-1 (SYNGAP1)	S1073	Uniprot
Q96PV0-1 (SYNGAP1)	T1077	Uniprot
Q96PV0-1 (SYNGAP1)	S1138	Uniprot
Q9BXL7 (CARD11)	S116	Uniprot
Q9NSC5 (HOMER3)	S120	Uniprot
Q9NSC5 (HOMER3)	S159	Uniprot
Q9NSC5 (HOMER3)	S176	Uniprot
Q9UBS5 (GABBR1)	S868	Uniprot
Q9UQL6 (HDAC5)	S259	Uniprot
Q9UQL6 (HDAC5)	S498	Uniprot
Q9UQM7-1 (CAMK2A)	T286	Uniprot
Q9UQM7-1 (CAMK2A)	T305	Uniprot
Q9UQM7 (CAMK2A)	T306	Uniprot
Q9UQM7 (CAMK2A)	S314	Uniprot
Q9Y496 (KIF3A)	S687	Uniprot
Q9Y4D2 (DAGLA)	S782	Uniprot
Q9Y4D2 (DAGLA)	S808	Uniprot

Research Backgrounds

Q9UQM7_KCC2A_HUMAN

Function:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity.

PTMs:

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.

Subcellular Location:

Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Dendrite.
Note: Postsynaptic lipid rafts.

Subunit Structure:

There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with BAALC. Interacts with MPDZ. Interacts with SYN1. Interacts with CAMK2N2. Interacts with SYNGAP1. Interacts with SYNPO2 (By similarity). Interacts with SHANK3. Interacts with GRIN2B. Interacts with CACNB2. Interacts with LRRC7. Interacts with GRM5.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

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CaMKII alpha Antibody - #AF6793