PFKFB3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from 293 cells(heat shock treatment), using PFKFB3 Antibody.

Product:	PFKFB3 Antibody
Catalog:	AF6581
Description:	Rabbit polyclonal antibody to PFKFB3
Application:	WB IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	53kDa; 60kD(Calculated).
Uniprot:	Q16875
RRID:	AB_2847305

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

PFKFB3 Antibody detects endogenous levels of total PFKFB3.

RRID:

AB_2847305
Cite Format: Affinity Biosciences Cat# AF6581, RRID:AB_2847305.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK;

Immunogens

Immunogen:

A synthesized peptide derived from human PFKFB3.

Uniprot:

Q16875(F263_HUMAN) >>Visit HPA database.

Gene(ID):

PFKFB3(5209)

Expression:

Q16875 F263_HUMAN:

Ubiquitous.

Sequence:

Q16875 F263_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

PTMs - Q16875 As Substrate

Q16875

Site	PTM Type	Enzyme	Source
K12	Ubiquitination		Uniprot
S22	Phosphorylation		Uniprot
K97	Ubiquitination		Uniprot
K100	Ubiquitination		Uniprot
K111	Ubiquitination		Uniprot
K116	Ubiquitination		Uniprot
S172	Phosphorylation		Uniprot
K176	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
Y194	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
K214	Ubiquitination		Uniprot
S269	Phosphorylation	O14920 (IKBKB)	Uniprot
S273	Phosphorylation		Uniprot
K277	Ubiquitination		Uniprot
S283	Phosphorylation		Uniprot
K284	Ubiquitination		Uniprot
K292	Ubiquitination		Uniprot
K302	Ubiquitination		Uniprot
K319	Ubiquitination		Uniprot
K352	Ubiquitination		Uniprot
K411	Ubiquitination		Uniprot
S461	Phosphorylation	Q15418 (RPS6KA1) , P17612 (PRKACA) , Q13131 (PRKAA1) , P17252 (PRKCA) , P49137 (MAPKAPK2) , P31749 (AKT1)	Uniprot
T463	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
T471	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot

Research Backgrounds

Q16875_F263_HUMAN

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

PTMs:

Phosphorylation by AMPK stimulates activity.

Tissue Specificity:

Ubiquitous.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PFKFB3 Antibody - #AF6581