Phospho-HER3/ErbB3 (Tyr1289) Antibody - #AF3802

Product: Phospho-HER3/ErbB3 (Tyr1289) Antibody
Catalog: AF3802
Description: Rabbit polyclonal antibody to Phospho-HER3/ErbB3 (Tyr1289)
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 148kD(Calculated).
Uniprot: P21860
RRID: AB_2847116

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-HER3/ErbB3 (Tyr1289) Antibody detects endogenous levels of HER3/ErbB3 only when phosphorylated at Tyr1289.
RRID:
AB_2847116
Cite Format: Affinity Biosciences Cat# AF3802, RRID:AB_2847116.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c erbB 3; c erbB3; Erb b2 receptor tyrosine kinase 3; ErbB 3; ERBB3; ERBB3 protein; erbB3 S; ERBB3_HUMAN; Glial growth factor receptor; HER 3; HER3; Human epidermal growth factor receptor 3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c ErbB 3; proto-oncogene-like protein c ErbB3; Proto-oncogene-like protein c-ErbB-3; Receptor tyrosine protein kinase erbB 3; Receptor tyrosine protein kinase erbB3; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase type cell surface receptor HER3; Tyrosine kinase-type cell surface receptor HER3; v erb b2 avian erythroblastic leukemia viral oncogene homolog 3; v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian); v erb b2 erythroblastic leukemia viral oncogene homolog 3;

Immunogens

Immunogen:

A synthesized peptide derived from human HER3/ErbB3 around the phosphorylation site of Tyr1289.

Uniprot:

P21860(ERBB3_HUMAN) >>Visit HPA database.

Gene(ID):
ERBB3(2065)
Expression:
P21860 ERBB3_HUMAN:

Epithelial tissues and brain.

Sequence:
MRANDALQVLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSEKANKVLARIFKETELRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSFQAVTDHMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGPQLLLNWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADLLPPDDKQLLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGLRLAEVPDLLEKGERLAQPQICTIDVYMVMVKCWMIDENIRPTFKELANEFTRMARDPPRYLVIKRESGPGIAPGPEPHGLTNKKLEEVELEPELDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLLSPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAELQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT

PTMs - P21860 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
N250 N-Glycosylation
N353 N-Glycosylation
N408 N-Glycosylation
N414 N-Glycosylation
N437 N-Glycosylation
N469 N-Glycosylation
N522 N-Glycosylation
N566 N-Glycosylation
T640 Phosphorylation
Y680 Phosphorylation
S686 Phosphorylation
S693 Phosphorylation
S717 Phosphorylation
K736 Methylation
S844 Phosphorylation
S846 Phosphorylation
Y868 Phosphorylation
S869 Phosphorylation
S982 Phosphorylation
T996 Phosphorylation
Y1054 Phosphorylation
S1083 Phosphorylation
S1094 Phosphorylation
S1104 Phosphorylation
S1113 Phosphorylation
Y1132 Phosphorylation
S1147 Phosphorylation
Y1159 Phosphorylation
T1164 Phosphorylation
Y1197 Phosphorylation
Y1199 Phosphorylation
S1207 Phosphorylation
S1215 Phosphorylation
Y1222 Phosphorylation
Y1260 Phosphorylation
Y1262 Phosphorylation
Y1276 Phosphorylation P00533 (EGFR)
S1285 Phosphorylation
Y1289 Phosphorylation P00533 (EGFR)
Y1307 Phosphorylation Q01973 (ROR1)
T1312 Phosphorylation
S1315 Phosphorylation
T1319 Phosphorylation
Y1328 Phosphorylation
S1331 Phosphorylation

Research Backgrounds

Function:

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation.

PTMs:

Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Epithelial tissues and brain.

Subunit Structure:

Monomer and homodimer. Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5. Interacts with GRB7. Interacts with MUC1. Interacts with MYOC (By similarity). Interacts with isoform 2 of PA2G4. Found in a ternary complex with NRG1 and ITGAV:ITGB3 or ITGA6:ITGB4.

Family&Domains:

The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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