Phospho-Hexokinase 1 (Tyr732) Antibody - #AF3709

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Product: Phospho-Hexokinase 1 (Tyr732) Antibody
Catalog: AF3709
Description: Rabbit polyclonal antibody to Phospho-Hexokinase 1 (Tyr732)
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 102kD(Calculated).
Uniprot: P19367
RRID: AB_2847023

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 100ul $350 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Hexokinase 1 (Tyr732) Antibody detects endogenous levels of Hexokinase 1 only when phosphorylated at Tyr732.
RRID:
AB_2847023
Cite Format: Affinity Biosciences Cat# AF3709, RRID:AB_2847023.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BB404130; Brain form hexokinase; dea; DrHXK1; EC 2.7.1.1; Glycolytic enzyme; HEXOKIN; hexokinase I; Hexokinase PI; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; Hexokinase-A; HK I; HK1; HK1 tb; Hk1-s; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; HXK1_HUMAN; im:7148527; mHk1-s; wu:fc09d08; wu:fc16e02; wu:fc21e02; wu:fq14b11; zgc:55790; zgc:77618;

Immunogens

Immunogen:

A synthesized peptide derived from human Hexokinase 1 around the phosphorylation site of Tyr732.

Uniprot:

P19367(HXK1_HUMAN) >>Visit HPA database.

Gene(ID):
HK1(3098)
Expression:
P19367 HXK1_HUMAN:

Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).

Sequence:
MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS

PTMs - P19367 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y25 Phosphorylation
Y27 Phosphorylation
K62 Ubiquitination
K77 Acetylation
S124 Phosphorylation
K147 Ubiquitination
S155 Phosphorylation
K173 Ubiquitination
K176 Methylation
K176 Ubiquitination
S178 Phosphorylation
K187 Ubiquitination
K191 Ubiquitination
S269 Phosphorylation
K290 Ubiquitination
S298 Phosphorylation
Y301 Phosphorylation
K315 Ubiquitination
K333 Ubiquitination
S337 Phosphorylation
S340 Phosphorylation
K344 Ubiquitination
K346 Ubiquitination
S364 Phosphorylation
T403 Phosphorylation
Y417 Phosphorylation
K418 Ubiquitination
T419 Phosphorylation
Y461 Phosphorylation
K489 Acetylation
K501 Ubiquitination
T503 Phosphorylation
K510 Ubiquitination
K544 Acetylation
K624 Ubiquitination
Y686 Phosphorylation
R727 Methylation
Y732 Phosphorylation P12931 (SRC)
S733 Phosphorylation
K738 Ubiquitination
Y749 Phosphorylation
K763 Acetylation
K763 Ubiquitination
K777 Ubiquitination
S792 Phosphorylation
C813 S-Nitrosylation
T821 Phosphorylation
C823 S-Nitrosylation
S827 Phosphorylation
R849 Methylation
K880 Ubiquitination
K885 Ubiquitination
S893 Phosphorylation
T913 Phosphorylation

Research Backgrounds

Function:

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.

Subcellular Location:

Mitochondrion outer membrane>Peripheral membrane protein. Cytoplasm>Cytosol.
Note: The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.

Subunit Structure:

Monomer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2. Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).

Family&Domains:

The N- and C-terminal halves of this hexokinase contain a hexokinase domain (PubMed:9493266, PubMed:9735292, PubMed:10574795). The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus (PubMed:9493266, PubMed:9735292, PubMed:10574795). Each domain can bind a single D-glucose and D-glucose 6-phosphate molecule (PubMed:9493266).

Belongs to the hexokinase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Biosynthesis of other secondary metabolites > Neomycin, kanamycin and gentamicin biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

References

1). Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia. Cell Reports (PubMed: 35263589) [IF=8.8]

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