Phospho-TIRAP (Tyr86) Antibody | Affinity Biosciences

Product:	Phospho-TIRAP (Tyr86) Antibody
Catalog:	AF3647
Description:	Rabbit polyclonal antibody to Phospho-TIRAP (Tyr86)
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	24kD(Calculated).
Uniprot:	P58753
RRID:	AB_2846961

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-TIRAP (Tyr86) Antibody detects endogenous levels of TIRAP only when phosphorylated at Tyr86.

RRID:

AB_2846961
Cite Format: Affinity Biosciences Cat# AF3647, RRID:AB_2846961.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adapter protein wyatt; FLJ42305; MAL; MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt;

Immunogens

Immunogen:

A synthesized peptide derived from human TIRAP around the phosphorylation site of Tyr86.

Uniprot:

P58753(TIRAP_HUMAN) >>Visit HPA database.

Gene(ID):

TIRAP(114609)

Expression:

P58753 TIRAP_HUMAN:

Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.

Sequence:

P58753 TIRAP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS

PTMs - P58753 As Substrate

P58753

Site	PTM Type	Enzyme	Source
S3	Phosphorylation		Uniprot
T28	Phosphorylation	Q9NWZ3 (IRAK4) , P51617 (IRAK1)	Uniprot
Y86	Phosphorylation	Q06187 (BTK)	Uniprot
Y106	Phosphorylation	Q06187 (BTK)	Uniprot
T124	Phosphorylation		Uniprot
Y159	Phosphorylation		Uniprot
Y187	Phosphorylation	Q06187 (BTK)	Uniprot
Y216	Phosphorylation		Uniprot
T219	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot

Research Backgrounds

P58753_TIRAP_HUMAN

Function:

Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.

PTMs:

Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK.

Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.

Subcellular Location:

Cytoplasm. Cell membrane. Membrane.
Note: Colocalizes with DAB2IP at the plasma membrane.

Tissue Specificity:

Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.

Subunit Structure:

Homodimer. Also forms heterodimers with MYD88. May interact with PIK3AP1 (By similarity). Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with BMX and TBK1. Interacts with EIF2AK2. Does not interact with IRAK1, nor TLR9.

(Microbial infection) In case of infection, interacts with Brucella protein BtpA.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-TIRAP (Tyr86) Antibody - #AF3647