Phospho-PFKFB3 (Ser461) Antibody - #AF3581

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Product: Phospho-PFKFB3 (Ser461) Antibody
Catalog: AF3581
Description: Rabbit polyclonal antibody to Phospho-PFKFB3 (Ser461)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 55kDa; 60kD(Calculated).
Uniprot: Q16875
RRID: AB_2846895

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-PFKFB3 (Ser461) Antibody detects endogenous levels of PFKFB3 only when phosphorylated at Ser461.
RRID:
AB_2846895
Cite Format: Affinity Biosciences Cat# AF3581, RRID:AB_2846895.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK;

Immunogens

Immunogen:

A synthesized peptide derived from human PFKFB3 around the phosphorylation site of Ser461.

Uniprot:

Q16875(F263_HUMAN) >>Visit HPA database.

Gene(ID):
PFKFB3(5209)
Expression:
Q16875 F263_HUMAN:

Ubiquitous.

Sequence:
MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

PTMs - Q16875 As Substrate

Site PTM Type Enzyme
K12 Ubiquitination
S22 Phosphorylation
K97 Ubiquitination
K100 Ubiquitination
K111 Ubiquitination
K116 Ubiquitination
S172 Phosphorylation
K176 Ubiquitination
K189 Ubiquitination
Y194 Phosphorylation
K205 Ubiquitination
K214 Ubiquitination
S269 Phosphorylation O14920 (IKBKB)
S273 Phosphorylation
K277 Ubiquitination
S283 Phosphorylation
K284 Ubiquitination
K292 Ubiquitination
K302 Ubiquitination
K319 Ubiquitination
K352 Ubiquitination
K411 Ubiquitination
S461 Phosphorylation Q15418 (RPS6KA1) , P17612 (PRKACA) , Q13131 (PRKAA1) , P17252 (PRKCA) , P49137 (MAPKAPK2) , P31749 (AKT1)
T463 Phosphorylation
S467 Phosphorylation
T471 Phosphorylation
S478 Phosphorylation

Research Backgrounds

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

PTMs:

Phosphorylation by AMPK stimulates activity.

Tissue Specificity:

Ubiquitous.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

References

1). AICAR enhances the cytotoxicity of PFKFB3 inhibitor in an AMPK signaling-independent manner in colorectal cancer cells. Medical Oncology, 2021 (PubMed: 34761330) [IF=3.4]

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