CD64 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from C6 cells, using CD64 Antibody.

Product:	CD64 Antibody
Catalog:	DF13670
Description:	Rabbit polyclonal antibody to CD64
Application:	WB IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	42kDa; 43kD(Calculated).
Uniprot:	P12314
RRID:	AB_2846689

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

CD64 Antibody detects endogenous levels of total CD64.

RRID:

AB_2846689
Cite Format: Affinity Biosciences Cat# DF13670, RRID:AB_2846689.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD64; CD64A; Fc fragment of IgG high affinity Ia receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma RI; Fc gamma RIA; Fc-gamma RI; Fc-gamma RIA; FCG1; FcgammaRIa; FCGR1; FCGR1_HUMAN; FCGR1A; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I; Immunoglobulin G Fc receptor I; OTTHUMP00000013913;

Immunogens

Immunogen:

Uniprot:

P12314(FCGR1_HUMAN) >>Visit HPA database.

Gene(ID):

FCGR1A(2209)

Expression:

P12314 FCGR1_HUMAN:

Monocyte/macrophage specific.

Sequence:

P12314 FCGR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWFLTTLLLWVPVDGQVDTTKAVITLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPTPVWFHVLFYLAVGIMFLVNTVLWVTIRKELKRKKKWDLEISLDSGHEKKVISSLQEDRHLEEELKCQEQKEEQLQEGVHRKEPQGAT

PTMs - P12314 As Substrate

P12314

Site	PTM Type	Source
N59	N-Glycosylation	Uniprot
T73	Phosphorylation	Uniprot
S74	Phosphorylation	Uniprot
S76	Phosphorylation	Uniprot
N78	N-Glycosylation	Uniprot
Y133	Phosphorylation	Uniprot
Y137	Phosphorylation	Uniprot
Y138	Phosphorylation	Uniprot
N152	N-Glycosylation	Uniprot
N159	N-Glycosylation	Uniprot
N163	N-Glycosylation	Uniprot
N195	N-Glycosylation	Uniprot
S328	Phosphorylation	Uniprot
S340	Phosphorylation	Uniprot

Research Backgrounds

P12314_FCGR1_HUMAN

Function:

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

PTMs:

Phosphorylated on serine residues.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Stabilized at the cell membrane through interaction with FCER1G.

Tissue Specificity:

Monocyte/macrophage specific.

Subunit Structure:

Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN.

Family&Domains:

Belongs to the immunoglobulin superfamily. FCGR1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CD64 Antibody - #DF13670