Histone H2A-Bbd Antibody | Affinity Biosciences

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Western blot analysis of extracts from Recombinant H2ABbd, using Histone H2A-Bbd Antibody.

Product:	Histone H2A-Bbd Antibody
Catalog:	DF13466
Description:	Rabbit polyclonal antibody to Histone H2A-Bbd
Application:	WB
Reactivity:	Human
Mol.Wt.:	12kDa; 13kD(Calculated).
Uniprot:	P0C5Y9
RRID:	AB_2846485

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Histone H2A-Bbd Antibody detects endogenous levels of total Histone H2A-Bbd.

RRID:

AB_2846485
Cite Format: Affinity Biosciences Cat# DF13466, RRID:AB_2846485.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

P0C5Y9(H2AB1_HUMAN) >>Visit HPA database.

Gene(ID):

H2AFB1(474382)

Expression:

P0C5Y9 H2AB1_HUMAN:

Present in mature sperm.

Sequence:

P0C5Y9 H2AB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRRRRRRGSSGAGGRGRTCSRTVRAELSFSVSQVERSLREGHYAQRLSRTAPVYLAAVIEYLTAKVPELAGNEAQNSGERNITPLLLDMVVHNDRLLSTLFNTTTISQVAPGED

PTMs - P0C5Y9 As Substrate

P0C5Y9

Site	PTM Type	Enzyme	Source
S11	Phosphorylation		Uniprot
T19	Phosphorylation		Uniprot

Research Backgrounds

P0C5Y9_H2AB1_HUMAN

Function:

Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing.

Subcellular Location:

Nucleus. Chromosome.
Note: Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies.

Tissue Specificity:

Present in mature sperm.

Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. May be incorporated into a proportion of nucleosomes, replacing one or more H2A molecules.

Family&Domains:

The docking domain is responsible for the weaker heterodimerization with H2B.

Belongs to the histone H2A family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

Restrictive clause

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For Research Use Only.
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Histone H2A-Bbd Antibody - #DF13466