Transferrin Antibody | Affinity Biosciences

WB
IF/ICC
Cites

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Western blot analysis of extracts from HepG2 cells, using Transferrin Antibody.

Figure 8 WFA induces HO-1 expression, resulting in ameliorated ferroptosis in hemin-induced SH-SY5Y neuroblast injury in vitro.

Product:	Transferrin Antibody
Catalog:	DF13383
Description:	Rabbit polyclonal antibody to Transferrin
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	77kDa; 77kD(Calculated).
Uniprot:	P02787
RRID:	AB_2846402

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(86%)

Clonality:

Polyclonal

Specificity:

Transferrin Antibody detects endogenous levels of total Transferrin.

RRID:

AB_2846402
Cite Format: Affinity Biosciences Cat# DF13383, RRID:AB_2846402.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apotransferrin; Beta 1 metal binding globulin; Beta-1 metal-binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin; Serotransferrin precursor; Siderophilin; TF; TFQTL1; Transferin; Transferrin; TRFE_HUMAN;

Immunogens

Immunogen:

Uniprot:

P02787(TRFE_HUMAN) >>Visit HPA database.

Gene(ID):

TF(7018)

Expression:

P02787 TRFE_HUMAN:

Expressed by the liver and secreted in plasma.

Sequence:

P02787 TRFE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02787 As Substrate

P02787

Site	PTM Type	Source
T36	Phosphorylation	Uniprot
K37	Acetylation	Uniprot
C58	S-Nitrosylation	Uniprot
S63	Phosphorylation	Uniprot
Y64	Phosphorylation	Uniprot
S136	Phosphorylation	Uniprot
C137	S-Nitrosylation	Uniprot
T139	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
Y155	Phosphorylation	Uniprot
Y207	Phosphorylation	Uniprot
C213	S-Nitrosylation	Uniprot
C246	S-Nitrosylation	Uniprot
Y257	Phosphorylation	Uniprot
C260	S-Nitrosylation	Uniprot
S298	Phosphorylation	Uniprot
S305	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
T340	Phosphorylation	Uniprot
T349	Phosphorylation	Uniprot
T355	Phosphorylation	Uniprot
K359	Acetylation	Uniprot
C364	S-Nitrosylation	Uniprot
C374	S-Nitrosylation	Uniprot
S389	Phosphorylation	Uniprot
C396	S-Nitrosylation	Uniprot
S468	Phosphorylation	Uniprot
T476	Phosphorylation	Uniprot
N491	N-Glycosylation	Uniprot
C503	S-Nitrosylation	Uniprot
K508	Acetylation	Uniprot
S520	Phosphorylation	Uniprot
C525	S-Nitrosylation	Uniprot
Y533	Phosphorylation	Uniprot
Y534	Phosphorylation	Uniprot
Y536	Phosphorylation	Uniprot
T537	Phosphorylation	Uniprot
K546	Acetylation	Uniprot
C582	S-Nitrosylation	Uniprot
Y593	Phosphorylation	Uniprot
C596	S-Nitrosylation	Uniprot
T645	Phosphorylation	Uniprot
T654	Phosphorylation	Uniprot
Y666	Phosphorylation	Uniprot
Y669	Phosphorylation	Uniprot
Y674	Phosphorylation	Uniprot
C684	S-Nitrosylation	Uniprot
S685	Phosphorylation	Uniprot
T686	Phosphorylation	Uniprot
S688	Phosphorylation	Uniprot
T694	Phosphorylation	Uniprot

Research Backgrounds

P02787_TRFE_HUMAN

Function:

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed by the liver and secreted in plasma.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the transferrin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage. Neural Regeneration Research, 2023 (PubMed: 36453416) [IF=6.1]

Application: WB Species: Mouse Sample:

Figure 8 WFA induces HO-1 expression, resulting in ameliorated ferroptosis in hemin-induced SH-SY5Y neuroblast injury in vitro. (A) Representative western blot bands and (B) relative density of HO-1 in different groups. (C) Cell viability detection in different groups. (D) Typical BODIPY 581/591 C11 fluorescence images and (E) fluorescence density of BODIPY 581/591 C11 in different groups. Green indicates lipid ROS and blue indicates nuclei. (F) Representative western blot bands and (G–I) quantification of the relative density of 4-HNE, TF and FTH1 band densities in different groups. *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Bonferroni correction). Data are expressed as the mean ± SEM (n = 3). 4-HNE: 4-Hydroxynonenal; FTH1: ferritin heavy chain 1; HO-1: heme oxygenase-1; TF: transferrin; WFA: withaferin A; ZnPP: zinc protoporphyrin, a specific HO-1 inhibitor.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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Transferrin Antibody - #DF13383